FastQCFastQC Report
Thu 16 Mar 2023
EGAF00004952045

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952045
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53789761
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTAT3156710.5868607596155707TruSeq Adapter, Index 19 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAACGATCTCGTATG2403800.4468880239122089TruSeq Adapter, Index 19 (97% over 39bp)
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG1272250.23652270921969704No Hit
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA1198280.22277102142171631No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT1163490.216303247750069No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA1090430.20272073713062233No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC894760.16634392556605707No Hit
GTGGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAG838780.155936740451403No Hit
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG809590.15051005710919593No Hit
GGGAGGTAAGAGTCAGAAGCTTATGTTGTTTATGCGGGGAAACGCCATAT806560.1499467528773738No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGTATGGCT792770.14738306794112732No Hit
GGGGGTCTTAGCTTTGGTTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT788900.14666360015988916No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC755530.14045981725034992No Hit
GGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCAGT738830.1373551371607693No Hit
GTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGTATGG690290.1283311149123715No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC680000.12641811143202514No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG661080.12290071339041644No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGG613710.1140942046572767No Hit
GGGTATTACTATGAAGAAGATTATTACAAATGCATGGGCTGTGACGATAA596920.11097279275883007No Hit
CTGCTGTTAGAGAAATGAATGAGCCTACAGATGATAGGATGTTTCATGTG592090.11007485234968788No Hit
GTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAG576800.10723230393234133No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG576560.1071876857753653No Hit
GGGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGG565380.10510922329623291No Hit
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG557890.10371676498060663No Hit
GGTGTATGCATCGGGGTAGTCCGAGTAACGTCGGGGCATTCCGGATAGGC557860.10371118771098461No Hit
GTTTGGATGTAAAGTGAAATATTAGTTGGCGGATGAAGCAGATAGTGAGG551650.1025566928992304No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA550990.10243399296754638No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)