FastQCFastQC Report
Sun 5 Mar 2023
EGAF00004952046

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952046
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences53789761
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCTCGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG3331750.6194022687700732Illumina Single End PCR Primer 1 (97% over 41bp)
CGAGATCTCGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGG2638610.4905413132436116Illumina Single End PCR Primer 1 (97% over 42bp)
CGAGATCTCGCGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCAT1924080.35770376447666313No Hit
CGAGATCTCGCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCT1173590.2181809285228094No Hit
CGAGATCTCGATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCC1020790.18977403524808376No Hit
CGAGATCTCGCTATTCTCTACAAACCACAAAGACATTGGAACACTATACC804290.14952473947597572No Hit
CGAGATCTCGGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACT751140.13964367679566378No Hit
CGAGATCTCGAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAG748810.13921050885502168No Hit
CGAGATCTCGAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC746280.13874015911689958No Hit
CGAGATCTCGCCGTCATCTACTCTACCATCTTTGCAGGCACACTCATCAC730020.135717278981775No Hit
CGAGATCTCGCTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC713250.13259958526307636No Hit
CGAGATCTCGCACTACTATAACCACCCTAACCCTGACTTCCCTAATTCCC696220.12943355520765373No Hit
CGAGATCTCGCCAAAATGAACGAAAATCTGTTCGCTTCATTCATTGCCCC611480.11367962761537462No Hit
CGAGATCTCGCTACCGTATGGCCCACCATAATTACCCCCATACTCCTTAC603910.11227229658075633No Hit
CGAGATCTCGCATGCCTATCATATAGTAAAACCCAGCCCATGACCCCTAA603670.11222767842378031No Hit
CGAGATCTCGCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCA582400.10827339426178154No Hit
CGAGATCTCGCCCAATACCAAACGCCCCTCTTCGTCTGATCCGTCCTAAT575790.10704453585506728No Hit
CGAGATCTCGCTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC571730.10628974536622314No Hit
CGAGATCTCGCTACTATAACCACCCTAACCCTGACTTCCCTAATTCCCCC553390.10288017453730647No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)