FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004952063

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952063
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49321490
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTA4354260.8828322096514116No Hit
GGGAGGGCGATGAGGACTAGGATGATGGCGGGCAGGATAGTTCAGACGGT3198610.6485225811304565No Hit
GGGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGG3138360.6363068106823212No Hit
GGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGT2877510.5834191140616393No Hit
GGGGGCTTCAATCGGGAGTACTACTCGATTGTCAACGTCAAGGAGTCGCA2504160.507721887558547No Hit
GGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGG2028830.41134807565627074No Hit
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCCGTCCCGATCTCGTAT1960370.39746771640516126TruSeq Adapter, Index 16 (97% over 40bp)
GGGGGTCTTAGCTTTGGCTCTCCTTGCAAAGTTATTTCTAGTTAATTCAT1896020.38442066531242264No Hit
GGGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTG1721500.34903649504506046No Hit
GGGGGAAGTATGTAGGAGTTGAAGATTAGTCCGCCGTAGTCGGTGTACTC1683660.34136438294950133No Hit
GTCGGGGCATTCCGGATAGGCCGAGAAAGTGTTGTGGGAAGAAAGTTAGA1605200.32545650993106656No Hit
GGGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTT1471140.2982756603663028No Hit
GGGTGGTTGTGTTGATTCAAATTATGTGTTTTTTGGAAAGTCATGTCAGT1436420.29123613256614916No Hit
GGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGGCAA1418580.28761904800524074No Hit
GGGACGATTAGTTTTAGCATTGGAGTAGGTTTAGGTTATGTACGTAGTCT1350120.2737386887541313No Hit
GTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATAGGA1346920.2730898843485872No Hit
GTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGGT1333550.2703790984416732No Hit
GGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATTTGG1330200.2696998813296192No Hit
GGGCTGTTAATTGTCAGTTCAGTGTTTTAATCTGACGCAGGCTTATGCGG1275510.2586114085361168No Hit
GGCGTAGGTTTGGTCTAGGGTGTAGCCTGAGAATAGGGGAAATCAGTGAA1261100.255689761197401No Hit
GCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTGGGAGAGATA1208650.24505545148777946No Hit
GGGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTAT1149790.2331215054533024No Hit
GGCAGGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTA1139390.23101289113528403No Hit
GCGGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTACTGGTTG1139110.23095612074979893No Hit
GGGCAGTGAGAGTGAGTAGTAGAATGTTTAGTGAGCCTAGGGTGTTGTGA1056250.214156141673741No Hit
GGGGCGGTGATGTAGAGGGTGATGGTAGATGTGGCGGGTTTTAGGGGCTC1053490.21359654787395918No Hit
GTGGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTA1033840.20961248332116486No Hit
GGCGGGTTTTAGGGGCTCTTTGGTGAAGAGTTTTATGGCGTCAGCGAAGG1032730.20938742929299176No Hit
GGGCAGGATAGTTCAGACGGTTTCCATTTCCTGAGCGTCTGAGATGTTAG1030970.2090305868699425No Hit
GGGGGGCATCCATATAGTCACTCCAGGTTTATGGAGGGTTCTTCTACTAT1022890.20739235574594359No Hit
GGGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGG1008020.2043774427739308No Hit
GGGGGCTTTGTATGATTATGGGCGTTGATTAGTAGTAGTTACTGGTTGAA1007910.2043551401224902No Hit
GGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGTTGTTGTTGATT986400.19999395800897338No Hit
GGCCAATTGATTTGATGGTAAGGGAGGGATCGTTGACCTCGTCTGTTATG985720.19985608707279523No Hit
GTGGTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAG979170.19852806555519714No Hit
GTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATAG965820.19582133467581775No Hit
GTGGGAAGAAAGTTAGATTTACGCCGATGAATATGATAGTGAAATGGATT957000.1940330675330368No Hit
GTCCGGAGAGTATATTGTTGAAGAGGATAGCTATTAGAAGGATTATGGAT950230.19266044071255753No Hit
GGGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAAT945790.19176022459986508No Hit
GGGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGTGTGAGGCGTA913290.18517080485605766No Hit
GGGGGTTGTTAGGGGGTCGGAGGAAAAGGTTGGGGAACAGCTAAATAGGT891840.1808217878251448No Hit
GTTGGTATAGAATGGGGTCTCCTCCTCCGGCGGGGTCGAAGAAGGTGGTG887750.1799925346943087No Hit
GGGTGTTGAGGGTTATGAGAGTAGCTATAATGAACAGCGATAGTATTATT861220.17461354066959453No Hit
GGGGGGTTCATAGTAGAAGAGCGATGGTGAGAGCTAAGGTCGGGGCGGTG858710.17410463471399587No Hit
GGGGGTTTTATATTGATAATTGTTGTGATGAAATTGATGGCCCCTAAGAT844250.1711728498064434No Hit
GTGGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGA828440.167967350540302No Hit
GTGGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGG809290.16408466167587393No Hit
GTGTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGAC757770.15363891074661368No Hit
GGCTGTGAATGTTATAATTAAGGAGATTTGTAGGGAGATTAGTATAGAGA751350.15233724690799083No Hit
AGGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAA729770.1479618721981027No Hit
GTCGGGTGTGTTATTATTCTGAATTTTGGGGGAGGTTATATGGGTTTAAT716130.1451963434194709No Hit
GGGGAGGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCT705560.143053261367408No Hit
GTGGGGATCAATAGAGGGGGAAATAGAATGATCAGTACTGCGGCGGGTAG699840.1418935234924979No Hit
GGTGGTTGGTGTAAATGAGTGAGGCAGGAGTCCGAGGAGGTTAGTTGTGG677520.1373681127638277No Hit
GGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAGGGTT663930.13461272155403253No Hit
GGGGGGTTCGATTCCTTCCTTTTTTGTCTAGATTTTATGTATACGGGTTC660150.13384632134998353No Hit
GGTCGGGTGTGTTATTATTCTGAATTTTGGGGGAGGTTATATGGGTTTAA659460.1337064229000381No Hit
GGGCGATTGATGAAAAGGCGGTTGAGGCGTCTGGTGAGTAGTGCATGGCT634580.1286619686469326No Hit
GGGGCTAGGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAA625720.12686559144908233No Hit
GGGTAGGCTATGTGTTTTGTCAGGGGGTTGAGAATGAGTGTGAGGCGTAT599130.12147443234176421No Hit
GTTGAGGTTGCGGTCTGTTAGTAGTATAGTGATGCCAGCAGCTAGGACTG598120.12126965345126434No Hit
GGGGTGTAGGTGTGCCTTGTGGTAAGAAGTGGGCTAGGGCATTTTTAATC589390.1194996339323893No Hit
GTGGGGGCAATGAATGAAGCGAACAGATTTTCGTTCATTTTGGTTCTCAG577340.11705647984276224No Hit
GGGGCTAGTTTTTGTCATGTGAGAAGAAGCAGGCCGGATGTCAGAGGGGT572590.1160934108032827No Hit
GGGTGGGGTTATTTTCGTTAATGTTAGTAAGGGTGGGGAAGCGAGGTTGA572520.11607921820691143No Hit
GGGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTG568100.11518305712175361No Hit
GGCGTTTGGTATTGGGTTATGGCAGGGGGTTTTATATTGATAATTGTTGT565850.11472686652410542No Hit
GGCTGGAGTGGTAAAAGGCTCAGAAAAATCCTGCGAAGAAAAAAATTTCT565760.11470861890019948No Hit
GCGGTTGCTTGCGTGAGGAAATACTTGATGGCAGCTTCTGTGGAACGAGG561160.11377596256722981No Hit
GGGGTAAGGCGAGGTTAGCGAGGCTTGCTAGAAGTCATCAAAAAGCTATT553850.11229385000331499No Hit
GGTGTGTGGTGGCCTTGGTATGTGCTTTCTCGTGTTACATCGCGCCATCA547490.11100435124729606No Hit
GGGGGTTGAGAATGAGTGTGAGGCGTATTATACCATAGCCGCCTAGTTTC544700.11043867490621229No Hit
GTGGTGCTGGAGTTTAAGTTGAGTAGTAGGAATGCGGTAGTAGTTAGGAT537340.10894642477346082No Hit
GTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTGGCTGCTTTTAGGCCT530060.10747039475084796No Hit
GGGGTGTTTAAGGGGTTGGCTAGGGTATAATTGTCTGGGTCGCCTAGGAG521430.10572065036964617No Hit
GGGGTAGGAGTCAGGTAGTTAGTATTAGGAGGGGGGTTGTTAGGGGGTCG520600.10555236672695817No Hit
GGGGTGGTGGTTGTGGTAAACTTTAATAGTGTAGGAAGCTGAATAATTTA519260.10528067988213657No Hit
GCCTGGTTCTAGGAATAATGGGGGAAGTATGTAGGAGTTGAAGATTAGTC510650.10353499052846943No Hit
GTGGGGATGATGAGGCTATTGTTTTTTGTGAATTCTTCGATAATGGCCCA509650.10333223915173691No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC505640.10251920613103943No Hit
GGTGGTGATTAGTCGGTTGTTGATGAGATATTTGGAGGTGGGGATCAATA504370.10226171188258912No Hit
GGCGGATGAAGCAGATAGTGAGGAAAGTTGAGCCAATAATGACGTGAAGT501830.10174672338568848No Hit
GCTCGGGGGAATAGGTTATGTGATTAGGAGTAGGGTTAGGATGAGTGGGA501560.10169198051397069No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)