FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004952064

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952064
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49321490
Sequences flagged as poor quality0
Sequence length151
%GC48

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGAGATCTCGGATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCG2079040.42152822228201137Illumina Single End PCR Primer 1 (97% over 41bp)
CGAGATCTCGAGTAGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAG1390920.28201094492481876No Hit
CGAGATCTCGCTAAATACTACCGTATGGCCCACCATAATTACCCCCATAC1315380.266695105926443No Hit
CGAGATCTCGATCACATGCCTATCATATAGTAAAACCCAGCCCATGACCC970120.1966931655957677No Hit
CGAGATCTCGACTAAATACTACCGTATGGCCCACCATAATTACCCCCATA937290.19003683789763853No Hit
CGAGATCTCGCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTATCA893330.18112388737647625No Hit
CGAGATCTCGCATAAATATTATACTAGCATTTACCATCTCACTTCTAGGA802410.1626897322039541No Hit
CGAGATCTCGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTAT800740.16235113740481075No Hit
CGAGATCTCGCACTACTATAACCACCCTAACCCTAACTTCCCTAATTCCC746950.15144514085036767No Hit
CGAGATCTCGAAATACTACCGTATGGCCCACCATAATTACCCCCATACTC717180.14540923236504008No Hit
CGAGATCTCGGTTGACTATTCTCTACAAACCACAAAGACATTGGAACACT676080.13707615078133284No Hit
CGAGATCTCGAATCACATGCCTATCATATAGTAAAACCCAGCCCATGACC675960.13705182061612495No Hit
CGAGATCTCGCACATGCCTATCATATAGTAAAACCCAGCCCATGACCCCT584480.11850412467263255No Hit
CGAGATCTCGGGTCTACAAGACGCTACTTCCCCTATCATAGAAGAGCTTA579720.1175390281193857No Hit
CGAGATCTCGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAAT566660.11489109513925877No Hit
CGAGATCTCGGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCA532900.10804620866076836No Hit
CGAGATCTCGCGCAAAATTAACCCCCTAATAAAATTAATTAACCACTCAT518210.1050677909365674No Hit
CGAGATCTCGTTTACATAAATATTATACTAGCATTTACCATCTCACTTCT511640.10373571439143464No Hit
CGAGATCTCGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAA506960.10278683794832638No Hit
CGAGATCTCGCTAGCATTTACCATCTCACTTCTAGGAATACTAGTATATC506470.10268748977372744No Hit
CGAGATCTCGAAATATTATACTAGCATTTACCATCTCACTTCTAGGAATA505860.10256381143392058No Hit
CGAGATCTCGGCCCACCATAATTACCCCCATACTCCTTACACTATTCCTC498680.10110805654898099No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)