FastQCFastQC Report
Sat 2 Jul 2022
EGAF00004952071

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952071
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63248921
Sequences flagged as poor quality0
Sequence length151
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTAT12152861.9214335688034898TruSeq Adapter, Index 15 (97% over 40bp)
ATCGGAAGAGCACACGTCTGAACTCCAGTCACATGTCAGAATCTCGTATG2500850.3953980495572407TruSeq Adapter, Index 15 (97% over 39bp)
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT732490.11581067129983134No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)