FastQCFastQC Report
Fri 27 May 2022
EGAF00004952674

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004952674
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences114805883
Sequences flagged as poor quality0
Sequence length35-151
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCGTATCGAAGGCCTTTTTGGACAGGTGGTGTGTGGTGGCCTTGGTATG2075200.18075728749893416No Hit
GTCGTTACCTAGAAGGTTGCCTGGCTGGCCCAGCTCGGCTCGAATAAGGA1876360.16343761756529498No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1797190.15654162949123435No Hit
CTCCGATTATGATGGGTATTACTATGAAGAAGATTATTACAAATGCATGG1466180.1277094833197703No Hit
GCCCTCTTGAACTCTCTCTTCAAAGTTCTTTTCAACTTTCCCTTACGGTA1406920.12254772693138034No Hit
GTTGGTTAGTAGGCCTAGTATGAGGAGCGTTATGGAGTGGAAGTGAAATC1315910.11462043282224484No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)