FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953294

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953294
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376
Sequences flagged as poor quality0
Sequence length35-300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN442147.152303754266214No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG3093.2956484641638224No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG2893.0823378839590445No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG2332.4850682593856654No Hit
GGGGTTAGTCCTTGCTATATTATGCTTGGTTATAATTTTTCATCTTTCCC1551.6531569965870307No Hit
GGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGT1271.3545221843003414No Hit
GGGGAATCTTAGTTCAACTTTAAATTTGCCCACAGAACCCTCTAAATCCC1251.3331911262798635No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC1221.3011945392491466No Hit
GGGGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAGT951.0132252559726962No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG880.938566552901024No Hit
GGGAAAAGAGAGCTCCTTCCTGTGTCCTCTGAGGATCTGACCCCAGCCTT770.8212457337883959No Hit
GGGGAGGGGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCC750.799914675767918No Hit
GGGTTCTTTGCTAAATAATAAACAGGGTTCTTGTCTGAAACTATTTAAAA740.7892491467576791No Hit
GGGCTAAAAAATCCCAAACATATAACTGAACTCCTCATACCAAACCATCCG730.7785836177474402No Hit
GGGATTGGCTTCCTAGGGTTTATCGTGTGAGCACACCACATATTTACAGT710.7572525597269625No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA700.7465870307167235No Hit
GGGGCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGGGCTTCTC680.7252559726962458No Hit
GGGGGAAACAGAAACAGAGAATTCACAAATAGGCCATTGCTCATTTTATG640.6825938566552902No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC640.6825938566552902No Hit
GGGAAATACCAGGGGAGTGAGAGACTGAGCACTCTCCTAGTGCCAACTGC630.6719283276450512No Hit
GGGATGGCTGGGTCAAATGGTATTTCTAGTTCTAGATCCCTGAGGAATCA630.6719283276450512No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG620.6612627986348123No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG580.6186006825938567No Hit
GGGGCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATCTCTGCTATG580.6186006825938567No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG580.6186006825938567No Hit
GGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA550.5866040955631399No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT490.5226109215017064No Hit
GGGGTGACCTACATGCATTGCTTCAGAAGAGGGGCCCCGGCCCGTGTACA480.5119453924914675No Hit
GGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTTCGTAGTCT460.4906143344709898No Hit
GGGAATATTCCAGAGAAACCAGCTGTTGTTCCAAATGTCGCCATTTGATA440.469283276450512No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCC440.469283276450512No Hit
GGGGCCTTTTTCTCATGCTTGTAAGCTCCTTCATCTGGAAATGTGATTTA420.44795221843003413No Hit
GGGGGAGGGTCGGAGACCCAGTACTTCGGGCCAGGCACGCGGCTCCTGGT400.42662116040955633No Hit
GGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT380.4052901023890785No Hit
GGGCTTTAGAGAAGTATCACAGTAACTAGAAAAGAGAGAGAACTCAGTAC380.4052901023890785No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA380.4052901023890785No Hit
GGGGATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTCTGCTGTGC380.4052901023890785No Hit
GGGGAAGGTTTCTGCAGTTTCCTTTTTCCAGTGATTTTCAAACAAACTTG380.4052901023890785No Hit
GGGACGAAATTGACCAACCCTGGGGTTAGTATAGCTTAGTTAAACTTTCG360.3839590443686007No Hit
GGGTCTTAGCTATTGTGTGTTCAGATATGTTAAAGCCACTTCGTAGTCTA350.37329351535836175No Hit
GGGGGAGTGAGGGAGTGAACACCAGATGGAAAGTCAAGCAACAGGTTTCT330.35196245733788395No Hit
GGGGACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGC330.35196245733788395No Hit
GGGGGTATACTCCAGTGGTAATGAAGATGGAAGGTTTACAGCACAGCTCA320.3412969283276451No Hit
GGGCTAGTTACGCGACCCCCGAGCGGTCGGCGTCCCCCAACTCCTCTAGA320.3412969283276451No Hit
GGGGGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGC300.3199658703071672No Hit
GGGAGAAGCCTCACACAGCCCAGTAACTTTGCTAGTACCTCTTGAGTGCA300.3199658703071672No Hit
GGGAGTCCATATAAGCGACACGGCTGAGTACTTCTGTGCTGTGAGGAATA280.2986348122866894No Hit
GGGATTTTTTAGGTAGTGGGTGTTGAGCTTGAACGCTTTCTTAATTGGTG270.2879692832764505No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT270.2879692832764505No Hit
GGGAGAAAGTCCAGTGGGAAAATGTGAGCACATCTATGTGTGCATAGGCT250.2666382252559727No Hit
GGGGGCAGAGTCCTCATTATTTAATCACTTCCTAAAATGCCCCACCCCTT250.2666382252559727No Hit
GGGGACTTCGGGCCGGGCACCAGGCTCACGGTCACAGGTCGCTGTGTTTGAGCCATCAGAAGCA250.2666382252559727No Hit
GGGTGGCATTCAGGATTCCCCACATGTACTGATCCACCTCCAGGCCCTCC240.25597269624573377No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA230.2453071672354949No Hit
GGGGGCACTTGGCCTCCAGAGGGCGATGCTGCACACACTGGAGCTTTTGT230.2453071672354949No Hit
GGGGGTGCTGAGAGGCGCTGCTGGGCGTCTGGGCGGAGGACTCCTGGTTC220.234641638225256No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT220.234641638225256No Hit
GGGGACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG220.234641638225256No Hit
GGGACACAGGTTGGTATCTTCGAACAGACCCACCAGGTACGCTTCGCTAG220.234641638225256No Hit
GGGGGGGATTCTGTGATCAGTCATCCCTCCTCGCTGGTGAATGGAGGCAG220.234641638225256No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCCCACC210.22397610921501707No Hit
GGGTCTCATGTGATCTGATGGTTTTATAAATGGGAGTCGCCCTACACAAA210.22397610921501707No Hit
GGGGGCATTCCTAATGCTTACCGAACGAAAAATTCTAGGCTATATACAAC210.22397610921501707No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTGAGCCATCAGAAGCA210.22397610921501707No Hit
GGGGCCTTTCTTTGCGGAATCACCATGGCGGCTGGGACCCTGTACACGTA210.22397610921501707No Hit
GGGCATCAATTGGAGGGGTTCCATCTTTGAATACAGAATTCAGGGGAGCC210.22397610921501707No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA200.21331058020477817No Hit
GGGGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGGACCTGA190.20264505119453924No Hit
GGGACTGTGCTCCTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCA190.20264505119453924No Hit
GGGGGCTGAAGACAGAGCGATGGTAGTTCTGGAGGCCTCGCTCCGGGGCC180.19197952218430034No Hit
GGGCAGACTGGTTGAGCTCAGGAGTTTGCAGCCAGCCTGGGCAACATGGC180.19197952218430034No Hit
GGGACTGTGGGAAAGGCGTCTGTGCTCCTTCCCAGAATTCTGCTTTGGGT170.18131399317406144No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC170.18131399317406144No Hit
GGGGGGCTGGCAAACTGCTTCTCCTGAGGTGTGTTTTATGAAGAGATACT160.17064846416382254No Hit
GGGGAGGGGTTGAGACCCATCTTTAAAAGTGGGGGACAGCAGGGACAAAG140.1493174061433447No Hit
GGGAGGAACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG130.1386518771331058No Hit
GGGTCATGCCGTGTTTCAGCTCATCAGTCAGGACTCGCCTGCCCACCATA120.12798634812286688No Hit
GGGTTCATTGGGATGACATGCCGACAATCAGGATCATTTTCCAGGGCTTT110.117320819112628No Hit
GGGCAGTATGTCCATAAACCAACAAATAATTTGGCTGTAATGTATCATAA110.117320819112628No Hit
GGGTCTGTCTCCAGCATCTCAATGCCTGAGCTTCCCTGTTCAGGAGACAG100.10665529010238908No Hit
GGGCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCA100.10665529010238908No Hit
GGGATACAAAGAAACCTCACCTTTTTTTTTTTTTTTTTTTCTGGAGACAG100.10665529010238908No Hit
GGGGAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTAAAGTC100.10665529010238908No Hit
GGGGGCGACAAACCTACCGAGCCTGGTGATAGCTGGTTGTCCAAGATAGA100.10665529010238908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)