FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953332

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953332
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences144619
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGACAAGGGACCATCTTGACTGTCCATCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC1860312.863455009369446No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN38762.680145762313389No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG19641.3580511551041012No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC18881.3054992774116818No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC15811.0932173504173035No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG12360.8546594845767154No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA8750.6050380655377232No Hit
GGGGACAAGGGACCACCTTGACTGTCCATCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC8570.5925915681895186No Hit
GGGGCTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCAGACTACA8110.5607838527441069No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT6560.4536056811345674No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA6500.4494568486851659No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC6320.43701035133696126No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT6300.4356274071871608No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA6250.4321700468126594No Hit
GGGGTCAGAGTGTCTATGTGGCTGAATCGTTTCCAGAAAAGACCTCCAGA6180.4273297422883577No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG6020.4162661890899536No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT5980.4135003007903526No Hit
GGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAG5370.37132050422143703No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA5320.36786314384693575No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT5210.3602569510230329No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG4610.31876862652901766No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT4510.31185390578001504No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG4480.3097794895553143No Hit
GGGGGCATTTGATTGGTTGAGCAGCCAACAGAAAGGAACTTTCCTGCATT4460.30839654540551376No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT4370.3021732967314115No Hit
GGGACATGAAGTGTCTACCTTCTGCAGACTACAGTGGCTCAGGAACCGGG4330.29940740843181046No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC4260.2945671039075087No Hit
GGGGACAAGGGACCATCTTGACTGTCCATCCAAATATCCAGAACCCTGAC4170.2883438552334064No Hit
GGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT4140.2862694390087056No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3940.2724399975107005No Hit
GGGGGACTCAGCAATGTACTTCTGTGCAATGAGAGAGGGCGATACTGGAG3890.26898263713619924No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT3860.2669082209114985No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA3840.26552527676169796No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG3780.26137644431229645No Hit
GGGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGA3720.25722761186289494No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA3620.2503128911138924No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA3570.2468555307393911No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA3570.2468555307393911No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT3410.235791977540987No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA3400.23510050546608677No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT3370.23302608924138601No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG3320.22956872886688473No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC3310.22887725679198445No Hit
GGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTG3200.22127106396808166No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA3170.21919664774338088No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC3150.2178137035935804No Hit
GGGGAAGACAATCTGCATCTTCACAGGAGGGATGGCCATGCTCCTGGGGG3140.21712223151868013No Hit
GGGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCA3040.21020751076967756No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG3020.20882456661987706No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG3020.20882456661987706No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC3010.2081330945449768No Hit
GGGGAGTTGAGGGTGAATTTACAGAGTTTCAGCTGGTTGGGAAGACTGGA3000.20744162247007653No Hit
GGGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTT2990.2067501503951763No Hit
GGGGGCATACAGTTAGAGAGAAAGAAAAATGTATCATCAAACCATAGAAT2980.20605867832027605No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT2940.20329279002067502No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG2920.20190984587087452No Hit
GGGGAGAGGAGAGAAGAGACAACCTGATGATAGAAGTAACTCTTATAACT2860.19776101342147298No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA2820.19499512512187195No Hit
GGGGATCAAGACCATTATTTGGGTAACACACTAAAGATGAACTATTCTCC2810.1943036530469717No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA2730.18877187644776966No Hit
GGGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC2730.18877187644776966No Hit
GGGGAGCTTTTGTAGTTTTAAATAAGCAAGGAGTTTCTCCAGCGAAACTT2650.18324009984856762No Hit
GGGATTATTTTTTTTTCGTGTTTAAGGTTTGAATCCTCAGTGAACCAGGG2590.1790912673991661No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT2570.17770832324936556No Hit
GGGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTA2550.17632537909956508No Hit
GGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAG2490.17217654665016355No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC2470.17079360250036302No Hit
GGGGGAGACTTCCACTAGAGGGGAAGGTGATCTCACTTGCTCAGCCTTCC2440.16871918627566226No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT2360.16318740967646023No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG2340.1618044655266597No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA2340.1618044655266597No Hit
GGGGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCCC2310.15973004930195894No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC2270.15696416100235794No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA2270.15696416100235794No Hit
GGGGGCATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCT2250.1555812168525574No Hit
GGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGT2220.15350680062785665No Hit
GGGGAGACTGCTGTCTGCACAAGAAGAAGACTTACAGATGCTTAAGGAGG2190.15143238440315587No Hit
GGGGGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTATGACT2180.15074091232825562No Hit
GGGGGTTGAATACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAA2170.15004944025335537No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT2170.15004944025335537No Hit
GGGGAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGG2160.14935796817845512No Hit
GGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTTC2150.14866649610355487No Hit
GGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCATGCCCTGG2140.1479750240286546No Hit
GGGAGAATTATGCTCTTTGCTGGCCTGGTCAGAAGGCAGCTTTGTCAGGA2140.1479750240286546No Hit
GGGGGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGAT2130.14728355195375434No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA2130.14728355195375434No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG2130.14728355195375434No Hit
GGGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGAC2080.14382619157925308No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG2060.14244324742945255No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG2010.13898588705495127No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA2000.13829441498005102No Hit
GGGAACCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCAT1990.1376029429051508No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA1970.13621999875535026No Hit
GGGGTTTCCAGACAAAGACAAGTGACAAGAATTAGAGGTTTAAAAAGCAA1970.13621999875535026No Hit
GGGACAAATGATTATATCACGTGGTACCAACAGTTTCCCAGCCAAGGACC1960.13552852668045No Hit
GGGGGGACTTGGGGAGGCAGGAGTGGAAGGCAGCAGGTCGCGGTTTTCCT1960.13552852668045No Hit
GGGAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTTTGCACCTAGA1950.13483705460554973No Hit
GGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATTT1950.13483705460554973No Hit
GGGGGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAATGA1920.13276263838084898No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA1890.13068822215614823No Hit
GGGGATATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGA1880.12999675008124797No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1830.1265393897067467No Hit
GGGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGC1800.12446497348204592No Hit
GGGCAACATCGAATACGCCGCAGGCCCCTTCGCCCTATTCTTCATAGCCG1800.12446497348204592No Hit
GGGGGTCATCCTAGCTACTGAAGAGGTTGAGGCAGGAGGATCACTTGTGC1800.12446497348204592No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA1790.12377350140714567No Hit
GGGGAGAGGAAAGAAAGGAATACCCGATGATGGAAGTAGCTCTTATGGCT1790.12377350140714567No Hit
GGGGAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAA1770.12239055725734517No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT1760.12169908518244492No Hit
GGGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAA1730.11962466895774414No Hit
GGGATCCATTCCAATCACTTCCATTCCATTCCATTACAGTCCATTCCAAT1720.11893319688284387No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1710.11824172480794364No Hit
GGGGACCTTTGGCAAAGGGACTCATCTAATCATCCAGCCCTATATCCAGA1710.11824172480794364No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1700.11755025273304338No Hit
GGGGACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGC1700.11755025273304338No Hit
GGGAAACATTAGGGCTGGGTTCATGTAAAGGGGACCAGCATTGTGCCGAC1680.11616730858324287No Hit
GGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCTAG1680.11616730858324287No Hit
GGGAGACTCTGCACCAGCTGTTCCCCTCCTTCCTGCAATGCTCTTCCCTT1670.1154758365083426No Hit
GGGAGTGTAGTGACTAACCCTCCTACTTTTGGTGTTGTGTTTCTGCCTAG1670.1154758365083426No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA1670.1154758365083426No Hit
GGGAGGCAGATGCTGCTGTTTACTACTGTGCTGTGGAGGATAGGGGAGGA1650.11409289235854211No Hit
GGGATCTCTGCTATGTTCATTTCTTTTTGGATTGAAAATTTTAATCCTCA1620.11201847613384133No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCCCAC1620.11201847613384133No Hit
GGGAGGGGAGTGGACACTGGGCAATCCAGGGCCCTCCTCGAGGGAAGCGG1610.11132700405894107No Hit
GGGAGTGCTTCTCAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGA1600.11063553198404083No Hit
GGGTAATAATCTTCTTCATAGTAATACCCATCATAATCGGAGGCTTTGGC1590.10994405990914058No Hit
GGGGACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGG1590.10994405990914058No Hit
GGGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTA1570.10856111575934006No Hit
GGGAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATCTCTGC1570.10856111575934006No Hit
GGGGATAGTGTCTCCAGAGCAAACACAGATGATTTCCCCCTCACGTTGGC1560.1078696436844398No Hit
GGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATTT1560.1078696436844398No Hit
GGGAGCTTTTGGCTATACATGCCGGGTAAACTCATCCACCTCTCTGTCAG1560.1078696436844398No Hit
GGGAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACAAG1560.1078696436844398No Hit
GGGGGCCTCCTCTGCCTCCTGGTTCAAAAGCAGCTAAACCAAAAGAAGCC1560.1078696436844398No Hit
GGGATGGAATGGAATGGAATGGAATGGAATGGAACGGAACGGAATGGAAT1550.10717817160953953No Hit
GGGGGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAAAACGTGT1530.10579522745973904No Hit
GGGGGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAAC1530.10579522745973904No Hit
GGGGGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTAT1530.10579522745973904No Hit
GGGGAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACCAA1520.10510375538483878No Hit
GGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATC1510.10441228330993853No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC1510.10441228330993853No Hit
GGGGGGGCTTTGTGTCGGGGAAAGCTGAGCTGTTAGGTTTTTGATGCTGA1510.10441228330993853No Hit
GGGGCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAG1500.10372081123503826No Hit
GGGTGTGATTGTGTGTCAGTAAAACTTTATTTATAAAAACAAGCAGTGGG1500.10372081123503826No Hit
GGGGATATTCCGAAATCCTCCAACAGAGACCTGTGTGAGCTTCTGCTGCA1500.10372081123503826No Hit
GGGAAAATTGCAGCTACTCAACCTGGAGACTCAGCTGTCTACTTTTGTGC1490.10302933916013803No Hit
GGGGAAAATGATGCTGTAAAATGGAAATGAGACCTTTAGAAAGAGAGATG1480.10233786708523777No Hit
GGGGACATTAAAAACACAAAATCCTACGGAAATACTGAAGAATGAGTCTC1480.10233786708523777No Hit
GGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCA1480.10233786708523777No Hit
GGGGACAGGAGGGATGGCCATGCTCCTGGGGGCATCAGTGCTGATTCTGT1470.10164639501033751No Hit
GGGGGAGTTTGACGCTGTGTGGAATATGGAAACAAACTGGTCTTTGGCGC1470.10164639501033751No Hit
GGGAAAAATGGAAATGAGACCTTTAGAAAGAGAGATGCTGAACATGAGAA1460.10095492293543726No Hit
GGGAGTGATGCCCTCTGCTAGGCCAGAGACACTAACAATGAACTCCTCTC1460.10095492293543726No Hit
GGGAGAGGAGAACCCATCAGAGCAGGAGACTTTTCACTCTGCAGGGGAGC1450.10026345086053699No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG1450.10026345086053699No Hit
GGGGGCACACATCTACTTTCCAAAATAGACCGCAACTATTTCTATTGATC1450.10026345086053699No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)