FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953375

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953375
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11381
Sequences flagged as poor quality0
Sequence length290-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA4173.6640014058518586No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT2191.924259731130832No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCATA1831.6079430629997367No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA1691.4849310253931993No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCATA1661.4585713030489411No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA1521.335559265442404No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCATA1251.0983217643440824No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCATA1221.0719620419998241No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT1141.001669449081803No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT1060.9313768561637817No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA1040.9138037079342765No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT990.8698708373605131No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTNAGTAAGATTACACAT930.8171513926719972No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA900.7907916703277392No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA840.7380722256392233No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA820.720499077409718No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCATA810.7117125032949653No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCATA800.7029259291802126No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCATA800.7029259291802126No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA800.7029259291802126No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANCTCTTAGTAAGATTACACAT780.6853527809507073No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT770.6765662068359547No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCATA760.667779632721202No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA760.667779632721202No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA750.6589930586064494No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA730.6414199103769441No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA730.6414199103769441No Hit
CGGATGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT730.6414199103769441No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA710.6238467621474387No Hit
CGGATGGTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT680.5974870398031807No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA610.5359810209999121No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANNTCTTAGTAAGATTACACAT610.5359810209999121No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCATA540.47447500219664357No Hit
CGGANGNTTNNGTCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT530.46568842808189087No Hit
CGGATGGTTNNGNCCTAGCCTTTCNATTANCTCTTAGTAAGATTACACAT530.46568842808189087No Hit
CGGANGNTTNNGNCCTAGCCTTTNNANTANCTCTNAGTAAGATTACACAT520.4569018539671382No Hit
CGGNTGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT500.43932870573763283No Hit
CGGANGNTTNNGNCCTAGCCTTTNNANTANCTCTTAGTAAGATTACACAT480.42175555750812754No Hit
CGGATGNTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT480.42175555750812754No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCATA480.42175555750812754No Hit
CGGANGNTTTNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT470.4129689833933749No Hit
CGGATGGTTNNGNCCTAGCCTTNCTATTANCTCTTAGTAAGATTACACAT450.3953958351638696No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA440.38660926104911697No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTANCTCTNAGTAAGATTACACAT440.38660926104911697No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA420.36903611281961163No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA410.360249538704859No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA400.3514629645901063No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANCTCTTAGTAAGATTACACAT400.3514629645901063No Hit
CGGATGNTTNNGNCCTAGCCTTNNNANTAGCTCTNAGTAAGATTACACAT370.32510324224584836No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG370.32510324224584836No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG360.31631666813109566No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA340.29874351990159037No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTAGCTCTTAGTAAGATTACACAT340.29874351990159037No Hit
CGGANGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT330.2899569457868377No Hit
CGGNTGGTTNNGNCCTAGCCTTNCNATTANNTCTTAGTAAGATTACACAT320.2811703716720851No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT320.2811703716720851No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCATA300.26359722344257974No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA300.26359722344257974No Hit
CTTGGTGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCATA300.26359722344257974No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA290.2548106493278271No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA270.23723750109832178No Hit
CGGATGNTTNNGTCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT270.23723750109832178No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANGGACAGGATTGACAGATTGA260.2284509269835691No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA260.2284509269835691No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA260.2284509269835691No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA260.2284509269835691No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC240.21087777875406377No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA240.21087777875406377No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG220.19330463052455849No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA220.19330463052455849No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA220.19330463052455849No Hit
CTTGGGGGANNCNTCTGCATCANTNTNCGNNGATACCACTGCTTCCGCGG210.18451805640980581No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACNGCTTCCGCGGACAGG200.17573148229505314No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCATA200.17573148229505314No Hit
CTTGGGGGANNCNTTCACTCTGNGNTNATNNCACTGCTTCCGCGGACAGG190.1669449081803005No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA170.14937175995079519No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA170.14937175995079519No Hit
CTTGGGGGANNCNTCTGCATCANNNTNCGNNGATACCACTGCTTCCGCGG170.14937175995079519No Hit
CGGATTGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT170.14937175995079519No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA170.14937175995079519No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA170.14937175995079519No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC160.14058518583604254No Hit
CTTGGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA160.14058518583604254No Hit
CTTGNGNGANNCNTCTGCATCACNCTNCGTTGATNCCACTGCTTCCGCGG150.13179861172128987No Hit
CTTGGTGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA150.13179861172128987No Hit
CTTGNGNGANNCNTCACCCGGCCNGGNCACGGACAGGATTGACAGATTGA150.13179861172128987No Hit
CTTNGGGGANNCNTTCACTCTGNNNTNATNCCACTGCTTCCGCGGACAGG150.13179861172128987No Hit
CTTNGGGGANNCNTCTGCATCANNNTNCGNTGATACCACTGCTTCCGCGG150.13179861172128987No Hit
CTTGGGGGANNCNTCACCCGGCCNNGNCACGGACAGGATTGACAGATTGA150.13179861172128987No Hit
CGGATTNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT140.12301203760653721No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA140.12301203760653721No Hit
CTTGGGNGANNCNTCACCCGGCCNGGNCANGGACAGGATTGACAGATTGA140.12301203760653721No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA140.12301203760653721No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA140.12301203760653721No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCATA140.12301203760653721No Hit
CTTGGGGGANNCNTCACCCGGCNCNGNCANNGACAGGATTGACAGATTGA140.12301203760653721No Hit
CTTGGGGGANNCNTTCACTCTGNNNTNATNNCACTGCTTCCGCGGACAGG130.11422546349178456No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA130.11422546349178456No Hit
CTTGNGNGANNCNTCTGCATCACNCTNCGTTGATACCACTGCTTCCGCGG130.11422546349178456No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA130.11422546349178456No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNNGATACCACTGCTTCCGCGG130.11422546349178456No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA130.11422546349178456No Hit
CTTGNGNGANNCCTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG120.10543888937703189No Hit
CTTGGGNGANNCNTCTGCATCACNCTNCGNTGATACCACTGCTTCCGCGG120.10543888937703189No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA120.10543888937703189No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA120.10543888937703189No Hit
CTTNGGGGANNCNTCACCCGGCNNNGNCANGGACAGGATTGACAGATTGA120.10543888937703189No Hit
CTTGGGNGANNCNTTCACTCTGCNTTNATNCCACTGCTTCCGCGGACAGG120.10543888937703189No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)