FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953383

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953383
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9060
Sequences flagged as poor quality0
Sequence length288-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC2873.1677704194260485No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA2222.4503311258278146No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC1271.401766004415011No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1251.379690949227373No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC1211.335540838852097No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1171.2913907284768211No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA1121.2362030905077264No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC1021.1258278145695364No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1001.1037527593818985No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC1001.1037527593818985No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA991.0927152317880795No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA981.0816777041942605No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA961.0596026490066226No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA921.0154525386313467No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA870.9602649006622517No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC870.9602649006622517No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA840.9271523178807948No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA780.8609271523178808No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC750.8278145695364238No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC670.739514348785872No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC660.728476821192053No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC630.695364238410596No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC620.6843267108167771No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC620.6843267108167771No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC620.6843267108167771No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC610.673289183222958No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC600.6622516556291391No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA590.6512141280353201No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA590.6512141280353201No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA590.6512141280353201No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA570.6291390728476821No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC550.6070640176600441No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATACGGATGAA550.6070640176600441No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATACGGATGAA540.5960264900662252No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC540.5960264900662252No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC530.5849889624724062No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATACGGATGAA520.5739514348785872No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC520.5739514348785872No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC500.5518763796909493No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA490.5408388520971302No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA490.5408388520971302No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC490.5408388520971302No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATACGGATGAA480.5298013245033113No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATACGGATGAA460.5077262693156733No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATACGGATGAA450.49668874172185434No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA430.4746136865342163No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA430.4746136865342163No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC430.4746136865342163No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA420.4635761589403974No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC420.4635761589403974No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGGGTTCTGGATATACGGATGAA410.4525386313465784No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATACGGATGAA400.44150110375275936No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA380.41942604856512145No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATACGGATGAA380.41942604856512145No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA380.41942604856512145No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATACGGATGAA350.3863134657836645No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA350.3863134657836645No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA330.3642384105960265No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC310.34216335540838855No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA300.33112582781456956No Hit
GTCTNTNAGCNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA290.3200883002207506No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA290.3200883002207506No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA270.2980132450331126No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG250.27593818984547464No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA250.27593818984547464No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA250.27593818984547464No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA240.26490066225165565No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA220.24282560706401765No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA210.2317880794701987No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANGGACAGGATTGACAGATTGA200.22075055187637968No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC200.22075055187637968No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA190.20971302428256072No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA190.20971302428256072No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA190.20971302428256072No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA170.18763796909492272No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG170.18763796909492272No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT170.18763796909492272No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA170.18763796909492272No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA160.17660044150110377No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA160.17660044150110377No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA160.17660044150110377No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA140.1545253863134658No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA140.1545253863134658No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA140.1545253863134658No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA130.1434878587196468No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG130.1434878587196468No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG130.1434878587196468No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT130.1434878587196468No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC120.13245033112582782No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT110.12141280353200883No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA110.12141280353200883No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC110.12141280353200883No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG110.12141280353200883No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA110.12141280353200883No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC100.11037527593818984No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC100.11037527593818984No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT100.11037527593818984No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA100.11037527593818984No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA100.11037527593818984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)