FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953428

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953428
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences60786186
Sequences flagged as poor quality0
Sequence length21
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGG1397370.22988282238994234No Hit
AGGCTCCGGGCCGGGGGGGGT788510.1297186173187441No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTC9146750.014.9854731
ACTGATC7579150.014.98374751
TACTACG7195800.014.981631
TAGCTCC9212800.014.980472
CGCTCAG6415550.014.9798581
AGGCTCC8897100.014.9761581
ACTACGC7293650.014.967212
GAGCGCT8357550.014.9639021
CTGCGCA3399700.014.9637881
AGCTCCT9254550.014.9610753
CTGATCG7603000.014.9601432
AGCGCTA8411250.014.9519852
CTACGCC7297700.014.9501143
CTCCTTA9270750.014.9378495
GCGCTAC8444900.014.9376613
TGATCGT7611800.014.9345223
CAGCGTA2911600.014.9295382
TACGCCC7281500.014.9286834
ATCGTTG7621850.014.9255435
GCTCCTT9260700.014.9234434
Produced by FastQC (version 0.11.5)