FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004953453

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953453
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences138919
Sequences flagged as poor quality0
Sequence length287-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA18651.3425089440609277No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA9590.6903303363830721No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA9140.6579373591805296No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA8120.5845132775214333No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA7580.5456417048783824No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA7440.5355638897487025No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA7410.5334043579351996No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA7330.5276456064325254No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA7310.5262059185568568No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA6310.4542215247734291No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA4570.3289686795902648No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4440.3196107083984192No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA4100.2951360145120538No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3880.2792994478796997No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA3860.2778597600040311No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA3730.2685017888121855No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA3700.2663422569986827No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA3670.2641827251851798No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3670.2641827251851798No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3670.2641827251851798No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3650.2627430373095113No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA3640.262023193371677No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA3570.2569842858068371No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA3510.2526652221798314No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA3480.2505056903663286No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA3470.2497858464284943No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA3460.24906600249066002No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3430.24690647067715718No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA3430.24690647067715718No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA3340.24042787523664869No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3180.22891037223130026No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3160.2274706843556317No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATCAGGCCTGA3150.22675084041779742No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA3040.21883255710162036No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2910.20947458590977477No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA2880.20731505409627193No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2700.19435786321525494No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA2500.1799609844585694No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGCTTTAA2480.17852129658290083No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA2480.17852129658290083No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA2380.17132285720455806No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA2320.16700379357755238No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2290.16484426176404957No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2260.16268472995054672No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA2250.16196488601271244No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATCAGGCCAGA2220.1598053541992096No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG2180.15692597844787248No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGTTTAA2100.15116722694519827No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGCTTCA2040.14684816331819261No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2020.14540847544252405No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTGGGTTTCA2000.1439687875668555No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1980.14252909969118696No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1970.14180925575335265No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1970.14180925575335265No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA1960.1410894118155184No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGAATGA1950.14036956787768412No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATACGGATGAA1940.13964972393984984No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGTTGCA1930.13892988000201556No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1890.13605050425067844No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1890.13605050425067844No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA1880.1353306603128442No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1870.13461081637500988No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1870.13461081637500988No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATAGGGCTGGA1830.13173144062367279No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1830.13173144062367279No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA1810.13029175274800422No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGCTTCA1770.12741237699666713No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA1770.12741237699666713No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1760.12669253305883285No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1740.1252528451831643No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGCTTCA1730.12453300124533001No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA1730.12453300124533001No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1720.12381315730749574No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA1710.12309331336966145No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1700.1223734694318272No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA1700.1223734694318272No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA1690.1216536254939929No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA1660.11949409368049006No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA1660.11949409368049006No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTTTCA1640.1180544058048215No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA1610.11589487399131869No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATTTGGTTTTA1520.10941627855081018No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA1500.10797659067514162No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAATGA1490.10725674673730735No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA1480.10653690279947307No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCAGA1460.10509721492380451No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATCTGGCTTTA1450.10437737098597025No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNCTTCTGGATATAGGACTTGA1450.10437737098597025No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA1440.10365752704813597No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA1440.10365752704813597No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAAAGA1420.1022178391724674No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA1400.10077815129679885No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTAA1390.1000583073589646No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)