FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953455

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953455
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61018
Sequences flagged as poor quality0
Sequence length35-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC8041.3176439739093382No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA7381.2094791700809597No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC3830.6276836343374086No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG3640.5965452817201481No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC3470.5686846504310203No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC3420.5604903471106887No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC3410.5588514864466223No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC3120.5113245271886984No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA3100.5080468058605657No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC3060.5014913632043004No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC2900.47526959257923895No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCATA2680.43921465796977943No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCATA2560.4195483300009833No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA2470.4047985840243863No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCATA2450.4015208626962536No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCATA2440.3998820020321872No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT2350.38513225605559015No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG2240.3671047887508604No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2210.36218820675866137No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA2100.3441607394539316No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2070.3392441574617326No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1950.3195778294929365No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1830.2999115015241404No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1830.2999115015241404No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC1790.2933560588678751No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1720.28188403421941066No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCATA1680.27532859156314526No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA1630.2671342882428136No Hit
CGGATGGTTNNGNATTGGGTTANGNCAGGNGGTTTTATATTGATAATTGT1620.2654954275787472No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1580.2589399849224819No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1580.2589399849224819No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCATA1560.2556622635943492No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1550.25402340293028286No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA1540.2523845422662165No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCATA1530.2507456816021502No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC1470.24091251761775215No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC1450.23763479628961948No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1440.23599593562555313No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCATA1390.22780163230522144No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1390.22780163230522144No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA1360.2228850503130224No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCATA1360.2228850503130224No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1350.22124618964895604No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC1330.21796846832082337No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG1300.21305188632862435No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCATA1290.211413025664558No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG1260.20649644367235898No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA1210.19830214035202726No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA1200.19666327968796093No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1190.19502441902389459No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA1190.19502441902389459No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG1190.19502441902389459No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG1170.19174669769576191No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC1160.19010783703169556No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG1140.1868301157035629No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCATA1130.18519125503949654No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA1110.18191353371136387No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCATA1110.18191353371136387No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1110.18191353371136387No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT1090.17863581238323117No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA1070.1753580910550985No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG1070.1753580910550985No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCATA1040.17044150906289945No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA1030.16880264839883313No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCTGAGAGTCCAGTTTGTATCTG1030.16880264839883313No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG1030.16880264839883313No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG990.16224720574256776No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA990.16224720574256776No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG960.15733062375036874No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA960.15733062375036874No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA960.15733062375036874No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTNAGTAAGATTACACAT950.1556917630863024No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC940.15405290242223604No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA920.15077518109410337No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA920.15077518109410337No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCATA900.14749745976597067No Hit
CTTGGGGGANNCNAAAAGAACANTNGAGTNAAAAGAACGCCACTGGAGGA880.144219738437838No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA870.14258087777377165No Hit
CGGATGGTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG860.14094201710970533No Hit
CGGATGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT820.13438657445343996No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG820.13438657445343996No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCATA820.13438657445343996No Hit
CGGANGNTTNNGNATTGGGTTATNGCNGGGGGTTTTATATTGATAATTGT790.12946999246124094No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANCTCTTAGTAAGATTACACAT790.12946999246124094No Hit
CGGNTGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG790.12946999246124094No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT770.12619227113310824No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG760.12455341046904193No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT750.12291454980497558No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA730.1196368284768429No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCATA720.11799796781277656No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA720.11799796781277656No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCNGAGANTCCAGTTTGTATCTG710.11635910714871021No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA710.11635910714871021No Hit
CGGATGNTTNNGNTGGTGTCGTTNNGNCCTGAGAGTCCAGTTTGTATCTG710.11635910714871021No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT710.11635910714871021No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG700.11472024648464388No Hit
CGGATGGTTNNGNATTGGGTTANNNCNGGNNGTTTTATATTGATAATTGT700.11472024648464388No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG690.11308138582057754No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG680.1114425251565112No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA680.1114425251565112No Hit
CGGATGNTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT660.10816480382837851No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNNAGAGTCCAGTTTGTATCTG660.10816480382837851No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA660.10816480382837851No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCATA650.10652594316431217No Hit
CGGNTGGTTNNGNATTGGGTTANNNCNGGNGGTTTTATATTGATAATTGT640.10488708250024582No Hit
CGGATGNTTNNGNATTGGGTTATNGCNGGNGGTTTTATATTGATAATTGT640.10488708250024582No Hit
CGGANGNTTNNGNTGGTGTCGTTNNGNCCNGAGAGTCCAGTTTGTATCTG620.10160936117211315No Hit
CGGATGGTTNNGNATAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC620.10160936117211315No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)