FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9376
Sequences flagged as poor quality0
Sequence length290-300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT3493.7222696245733786No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCCTT1491.5891638225255973No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT1461.5571672354948805No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT1391.4825085324232081No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCCTT1391.4825085324232081No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT1251.3331911262798635No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCCTT1171.2478668941979523No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCCTT1101.1732081911262797No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT941.0025597269624573No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT820.8745733788395904No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT810.8639078498293515No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT690.7359215017064846No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCCTT690.7359215017064846No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCCTT690.7359215017064846No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT680.7252559726962458No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT680.7252559726962458No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT680.7252559726962458No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTNAGTAAGATTACACAT670.7145904436860068No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT620.6612627986348123No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT610.6505972696245733No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT600.6399317406143344No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCCTT570.6079351535836177No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT550.5866040955631399No Hit
CGGATGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT550.5866040955631399No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT540.575938566552901No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCCTT540.575938566552901No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCCTT530.5652730375426621No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC520.5546075085324232No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCCTT520.5546075085324232No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANCTCTTAGTAAGATTACACAT510.5439419795221844No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT510.5439419795221844No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT480.5119453924914675No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA470.5012798634812287No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG430.458617747440273No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT420.44795221843003413No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT410.4372866894197952No Hit
CGGANGNTTNNGNCCTAGCCTTTNNANTANCTCTNAGTAAGATTACACAT390.41595563139931746No Hit
CGGANGNTTNNGNCCTAGCCTTTNNANTANCTCTTAGTAAGATTACACAT380.4052901023890785No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANNTCTTAGTAAGATTACACAT380.4052901023890785No Hit
CGGANGNTTNNGTCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT370.39462457337883955No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT360.3839590443686007No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT350.37329351535836175No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCCTT310.33063139931740615No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT310.33063139931740615No Hit
CGGATGGTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT300.3199658703071672No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA300.3199658703071672No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT290.30930034129692835No Hit
CGGNTGGTTNNGNCCTAGCCTTNCNATTANNTCTTAGTAAGATTACACAT280.2986348122866894No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT280.2986348122866894No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTANCTCTNAGTAAGATTACACAT280.2986348122866894No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC260.2773037542662116No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA260.2773037542662116No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTAGCTCTTAGTAAGATTACACAT250.2666382252559727No Hit
CGGATGNTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT250.2666382252559727No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC240.25597269624573377No Hit
CGGATGNTTNNGNCCTAGCCTTNNNANTAGCTCTNAGTAAGATTACACAT230.2453071672354949No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC220.234641638225256No Hit
CGGATGGTTNNGNCCTAGCCTTNCTATTANCTCTTAGTAAGATTACACAT220.234641638225256No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANCTCTTAGTAAGATTACACAT220.234641638225256No Hit
CGGATGNTTNNGTCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT220.234641638225256No Hit
CGGANGNTTTNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT210.22397610921501707No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT210.22397610921501707No Hit
TGCTTCTGANNGNTCAAACCCANTNCACCNTACTACCAGACAACCTTAGC210.22397610921501707No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA200.21331058020477817No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA200.21331058020477817No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG190.20264505119453924No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT180.19197952218430034No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCCTT180.19197952218430034No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA180.19197952218430034No Hit
CGGANGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT180.19197952218430034No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA180.19197952218430034No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA180.19197952218430034No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCCTT170.18131399317406144No Hit
CTTGGGGGANNCNTTCACTCTGNNNTNATNNCACTGCTTCCGCGGACAGG170.18131399317406144No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA170.18131399317406144No Hit
CGGATGGTTNNGNCCTAGCCTTTCNATTANCTCTTAGTAAGATTACACAT170.18131399317406144No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA170.18131399317406144No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC160.17064846416382254No Hit
CGGNTGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT160.17064846416382254No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA160.17064846416382254No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA160.17064846416382254No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC160.17064846416382254No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC150.1599829351535836No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC150.1599829351535836No Hit
CTTGGGGGANNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG150.1599829351535836No Hit
CTTGGGGGANNCNTTCACTCTGCNNTNATACCACTGCTTCCGCGGACAGG150.1599829351535836No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG150.1599829351535836No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA150.1599829351535836No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA150.1599829351535836No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA140.1493174061433447No Hit
CTTGGGGGANNCNTCTGCATCANTNTNCGNNGATACCACTGCTTCCGCGG140.1493174061433447No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA140.1493174061433447No Hit
CTTNGGGGANNCNTTCACTCTGNNNTNATNCCACTGCTTCCGCGGACAGG140.1493174061433447No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG140.1493174061433447No Hit
CTTGGGGGANNCNTTCACTCTGNGTTGATNCCACTGCTTCCGCGGACAGG140.1493174061433447No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC130.1386518771331058No Hit
TGCTTCTGANNGNTCAAACAAANTNACAANAAAAAAACAACCCCATCAAA130.1386518771331058No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT130.1386518771331058No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA130.1386518771331058No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC130.1386518771331058No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNNCACTGCTTCCGCGGACAGG120.12798634812286688No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA120.12798634812286688No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG120.12798634812286688No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA110.117320819112628No Hit
CTTGGGGGANNCNTTCACTCTGNGNTNATNNCACTGCTTCCGCGGACAGG110.117320819112628No Hit
CGGATTNTTNNGTCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT110.117320819112628No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA110.117320819112628No Hit
CGGATTGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT110.117320819112628No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA110.117320819112628No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC110.117320819112628No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC100.10665529010238908No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT100.10665529010238908No Hit
CGGATGGTTNNGNATGAGGTGTNGNTTCANAGAATGTAAGTTAATGTAAT100.10665529010238908No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC100.10665529010238908No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC100.10665529010238908No Hit
CGGANTGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT100.10665529010238908No Hit
CTTGGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT100.10665529010238908No Hit
CTTGNGNGANNCNTTCACTCTGCNNTNATNCCACNGCTTCCGCGGACAGG100.10665529010238908No Hit
GTCTCTCAGNNGNTACACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG100.10665529010238908No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA100.10665529010238908No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG100.10665529010238908No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACNGCTTCCGCGGACAGG100.10665529010238908No Hit
CTTGGTGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT100.10665529010238908No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)