FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953461

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953461
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences65271
Sequences flagged as poor quality0
Sequence length286-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA13162.01620934258706No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA10131.5519909301221062No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA6290.9636745262061251No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA5570.8533652004718787No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA5490.8411086087236291No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA5430.8319161649124419No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA5410.8288520169753795No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA5410.8288520169753795No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA5270.8074029814159427No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA4430.6787087680593219No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4400.6741125461537283No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4250.6511314366257603No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA4210.6450031407516355No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA4140.6342786229719172No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA4120.6312144750348547No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA4000.6128295874124803No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA3910.5990409216956994No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3260.49945611374117144No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA3010.46115426452789143No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2940.45042974674817304No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2790.427448637220205No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2750.42132034134608015No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2720.4167241194404866No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA2710.4151920454719554No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2650.4059996016607682No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA2650.4059996016607682No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA2640.404467527692237No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2640.404467527692237No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA2620.4014033797551746No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2530.3876147140383938No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2500.38301849213280015No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2490.381486418164269No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA2440.373826048321613No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA2430.37229397435308176No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA2290.350844938793645No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2260.34624871688805137No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA2050.31407516354889614No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2050.31407516354889614No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2020.30947894164330253No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA2010.30794686767477136No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1990.304882719737709No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1950.29875442386358414No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1940.2972223498950529No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1930.29569027592652175No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1930.29569027592652175No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1880.28802990608386575No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA1850.28343368417827214No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1810.27730538830414736No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1800.27577331433561614No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1800.27577331433561614No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1790.2742412403670849No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA1690.2589205006817729No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA1680.2573884267132417No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA1670.2558563527447105No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1630.24972805687058572No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA1630.24972805687058572No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1610.24666390893352333No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA1470.22521487337408652No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA1410.21602242956289933No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA1360.2083620597202433No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA1360.2083620597202433No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA1350.2068299857517121No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1240.18997717209786888No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA1220.1869130241608065No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA1200.18384887622374407No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA1170.1792526543181505No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA1160.17772058034961927No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA1150.17618850638108807No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA1110.17006021050696327No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA1090.16699606256990088No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1060.16239984066430727No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGCTTCA1030.15780361875871368No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA950.14554702701046407No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA910.13941873113633926No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATTCA890.13635458319927685No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA880.13482250923074565No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA870.13329043526221446No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA870.13329043526221446No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA840.12869421335662085No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA830.12716213938808965No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA830.12716213938808965No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGCTTCA830.12716213938808965No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGCTTCA820.12563006541955846No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA750.11490554763984005No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAATGA750.11490554763984005No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA750.11490554763984005No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA750.11490554763984005No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGGGTGA740.11337347367130884No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA740.11337347367130884No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGCTTCA730.11184139970277765No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGCTTCA730.11184139970277765No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAATGA720.11030932573424646No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAATGA720.11030932573424646No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA720.11030932573424646No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGCTTCA710.10877725176571525No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGAATGA710.10877725176571525No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGCTTCA710.10877725176571525No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA710.10877725176571525No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA710.10877725176571525No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA700.10724517779718405No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTTA700.10724517779718405No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA690.10571310382865284No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGCTTCA690.10571310382865284No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATTTGGCTTCA680.10418102986012165No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA680.10418102986012165No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA670.10264895589159044No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGCTTCA670.10264895589159044No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA670.10264895589159044No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGATGGA660.10111688192305925No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA660.10111688192305925No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)