FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953464

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953464
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71950
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC11091.5413481584433635No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC9941.3815149409312022No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT7381.0257123002084783No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA6250.8686587908269632No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG4550.6323835997220292No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT3780.5253648366921473No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT3690.5128561501042391No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG3690.5128561501042391No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG3610.501737317581654No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT3400.472550382209868No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT3130.43502432244614314No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC3020.41973592772758855No Hit
GGGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCAC3000.4169562195969423No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT3000.4169562195969423No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA2890.4016678248783877No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2840.3947185545517721No Hit
GGGGGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAATGA2820.3919388464211258No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC2700.37526059763724806No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT2470.34329395413481584No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT2430.33773453787352326No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA2330.3238359972202919No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA2330.3238359972202919No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA2160.3002084781097985No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA2110.29325920778318276No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG2100.2918693537178596No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC2030.28214037526059765No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT2030.28214037526059765No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA2010.27936066712995133No Hit
GGGAGGGCCCACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACT2010.27936066712995133No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGT1980.2751911049339819No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG1940.26963168867268933No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG1890.26268241834607364No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA1890.26268241834607364No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA1860.2585128561501042No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1810.25156358582348853No Hit
GGGAGGCTGACCGTACTGGGTAAGGAGGCGGTTGGGGCTCCGGAGAGCTC1760.24461431549687285No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1740.24183460736622656No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT1740.24183460736622656No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA1710.23766504517025713No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCG1680.2334954829742877No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG1650.2293259207783183No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA1650.2293259207783183No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT1610.2237665045170257No Hit
GGGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTT1540.21403752605976373No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA1420.19735927727588606No Hit
GGGAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCT1410.1959694232105629No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT1410.1959694232105629No Hit
GGGAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAA1410.1959694232105629No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC1410.1959694232105629No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG1390.1931897150799166No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1370.19041000694927032No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTC1360.1890201528839472No Hit
GGGGAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCT1360.1890201528839472No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT1340.1862404447533009No Hit
GGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAA1320.18346073662265464No Hit
GGGGGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGCCATGGGCACCA1240.1723419041000695No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG1240.1723419041000695No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG1240.1723419041000695No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT1230.17095205003474634No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT1220.1695621959694232No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC1200.16678248783877692No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG1190.16539263377345378No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1190.16539263377345378No Hit
GGGGGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTATCCTTTGGCTG1170.1626129256428075No Hit
GGGAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAAA1160.16122307157748436No Hit
GGGGGCGGTGTGGCTAGGGCTTCTCCTCAACTCTCTCTGGAAGGTTGCAG1160.16122307157748436No Hit
GGGAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACC1140.15844336344683807No Hit
GGGCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCA1140.15844336344683807No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT1120.1556636553161918No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT1120.1556636553161918No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC1090.15149409312022238No Hit
GGGGAGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTC1080.15010423905489925No Hit
GGGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTC1080.15010423905489925No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG1080.15010423905489925No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1080.15010423905489925No Hit
GGGAAAAACATTCAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGA1070.1487143849895761No Hit
GGGACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGC1070.1487143849895761No Hit
GGGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTT1050.1459346768589298No Hit
GGGGACTGTGCTCCTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTC1040.14454482279360667No Hit
GGGGGTTTTTGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTC1030.14315496872828354No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG1030.14315496872828354No Hit
GGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCAC1030.14315496872828354No Hit
GGGACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC1020.14176511466296038No Hit
GGGTGGGCAGCCCCTTCCCACTGTGCTCCTACAATGAGCAGTTCTTCGGG1020.14176511466296038No Hit
GGGAGCCCCTTCCCACTGTGCTCCTACAATGAGCAGTTCTTCGGGCCAGG1020.14176511466296038No Hit
GGGGGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCAGACT1010.14037526059763725No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1000.13898540653231412No Hit
GGGAGAGGAGTTTATGTAAAGGGTTGGCCCAGAGTGTGTATTCAGGAGGA990.13759555246699096No Hit
GGGGAGAAAGAAAATATCATAGAGGAGATTCGGCAGCTTATTTCTGTGCC990.13759555246699096No Hit
GGGGAAAATGATGCTGTAAAATGGAAATGAGACCTTTAGAAAGAGAGATG980.13620569840166782No Hit
GGGGGTTTTTCTGCTGTGGGTACGTGAGCAGGAAACATGGAGAAGAATCC980.13620569840166782No Hit
GGGGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCT980.13620569840166782No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC970.13481584433634466No Hit
GGGAGTCCTCTCACCACTGCAGACCAGAATCCTGCCCTGGGCCTTGCCTG970.13481584433634466No Hit
GGGATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACAAG960.13342599027102153No Hit
GGGATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTA960.13342599027102153No Hit
GGGACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGA960.13342599027102153No Hit
GGGAGCTTTTGGCTATACATGCCGGGTAAACTCATCCACCTCTCTGTCAG960.13342599027102153No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA950.1320361362056984No Hit
GGGGAGCTCACTGTCCTCCATCCTAGCCAGGGCCCTCTCCCCTCTCTATG940.13064628214037524No Hit
GGGGGCAGAAGCCTCACACAGCCCAGTAACTTTGCTAGTACCTCTTGAGT940.13064628214037524No Hit
GGGAAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGG940.13064628214037524No Hit
GGGGGGAATTATCGTAATTTGTTTCTAGGCTGAGATACCAGCATGGAGAA940.13064628214037524No Hit
GGGGAGCTTCTGGAAGGAAGGGCCCACACCAGCTCCTCTGGGGTTTGCCA920.12786657400972898No Hit
GGGGGAGAAAAGAAGGTTGGAATTATCGTAATTTGTTTCTAGGCTGAGAT900.12508686587908271No Hit
GGGAGTCAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCT900.12508686587908271No Hit
GGGGAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCAT890.12369701181375956No Hit
GGGACTCGACTCTCCATCCTAGAGGACCTGAACAAGGTGTTCCCACCCGA880.12230715774843642No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA880.12230715774843642No Hit
GGGGGATTATTGTAAAGCCCCATAGGACTGTGTGAATTATGGAGGAAGCC880.12230715774843642No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA880.12230715774843642No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC870.12091730368311328No Hit
GGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT870.12091730368311328No Hit
GGGGGATCCTGCCCCACGCTACGCTGAGAGACACTGCTGTGTACTATTGC870.12091730368311328No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT860.11952744961779013No Hit
GGGGGCATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGC860.11952744961779013No Hit
GGGAAACTGCAAAAAGCCCCGAGGCCTTGTTTGTAATGACTTTAAATGGG860.11952744961779013No Hit
GGGGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCT860.11952744961779013No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA850.118137595552467No Hit
GGGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGC850.118137595552467No Hit
GGGGATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGG820.11396803335649756No Hit
GGGGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTG820.11396803335649756No Hit
GGGGCATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTA820.11396803335649756No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA800.11118832522585129No Hit
GGGACTGTGCTCCTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCA800.11118832522585129No Hit
GGGTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCCTTCGCCAAACA800.11118832522585129No Hit
GGGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATG790.10979847116052814No Hit
GGGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAA790.10979847116052814No Hit
GGGGACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAG780.108408617095205No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT780.108408617095205No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT770.10701876302988186No Hit
GGGAAGGAGGTGCACAGTGGGGTCAGCACAGACCCGCAGCCCCTCAAGGA770.10701876302988186No Hit
GGGTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC770.10701876302988186No Hit
GGGAGAGCCCAGCACCTCACCCAGAGGACCCCAGTCAGAGGCCCCATCTC770.10701876302988186No Hit
GGGGGTATTACTGTGACAATAACAATGACATGCGCTTTGGAGCAGGGACC770.10701876302988186No Hit
GGGGGGAAATTGCAGTTTGGAGCAGGGACCCAGGTTGTGGTCACCCCAGA760.10562890896455872No Hit
GGGAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTTTGCACCTAGA760.10562890896455872No Hit
GGGGAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGC760.10562890896455872No Hit
GGGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGT760.10562890896455872No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA760.10562890896455872No Hit
GGGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAA760.10562890896455872No Hit
GGGGATTACTGTGACAATAACAATGACATGCGCTTTGGAGCAGGGACCAG760.10562890896455872No Hit
GGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTG760.10562890896455872No Hit
GGGGACTGTCCTCCATCCTAGCCAGGGCCCTCTCCCCTCTCTATGCCTTC750.10423905489923557No Hit
GGGGGGTGTTGTGTTTCTGCCTAGGGGTGAAGAGCCAAAAGATAGAACAG750.10423905489923557No Hit
GGGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGAT750.10423905489923557No Hit
GGGGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCCTGGTCCATA750.10423905489923557No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT750.10423905489923557No Hit
GGGAGAAGCCTCACACAGCCCAGTAACTTTGCTAGTACCTCTTGAGTGCA750.10423905489923557No Hit
GGGGAGCTTTTGGCTATACATGCCGGGTAAACTCATCCACCTCTCTGTCA740.10284920083391243No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA740.10284920083391243No Hit
GGGGAGGCTTTGTCGGGTGGAGCTGATTGGTTGCAGGAGCAGCAACAGTT720.10006949270326616No Hit
GGGGGAGACAAGACAATCTGCATCTTCACAGGAGGGATGGCCATGCTCCT720.10006949270326616No Hit
GGGAAAGCTGTAACATTGTGGGGACAGGGGAGGTTCACCCCTCCACTTTG720.10006949270326616No Hit
GGGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGT720.10006949270326616No Hit
GGGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT720.10006949270326616No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA720.10006949270326616No Hit
GGGGCTTTCTGAGCCAACCATGTGCCTCAGACTTCTCTGCTGTGTGGCCA720.10006949270326616No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)