FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004953467

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953467
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences415836
Sequences flagged as poor quality0
Sequence length288-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA62181.4953010321376696No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA28750.691378331842361No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA27530.6620398426302677No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA26200.6300560798006907No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA25730.6187535470714416No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA25630.616348752873729No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA23690.5696957454381054No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA23640.5684933483392491No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA23280.559836089227484No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA19570.47061822449234797No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA18110.43550822920574456No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA16100.38717186583172214No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA14470.34797372040900737No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA13640.32801392856799316No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA13320.32031858713531297No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA13190.31719235467828666No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA12860.3092565338258352No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA12810.3080541367269789No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA12760.3068517396281226No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA12740.3063707807885801No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA12730.30613030136880887No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA12690.3051683836897239No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA12420.2986754393558999No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA12130.2917015361825335No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA12080.29049913908367725No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA12000.2885753037255072No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA11870.28544907126848085No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA11830.2844871535893958No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA11820.28424667416962457No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA11740.2823228388114545No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA11700.2813609211323695No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA11700.2813609211323695No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA11420.2746274973787743No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA11400.2741465385392318No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA11250.270539347242663No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA11200.2693369501438067No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA11170.2686155118844929No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA11100.2669321559460941No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA11040.2654892794274666No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA11000.2645273617483816No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGATGA10930.2628440058099828No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA10770.25899633509364267No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC10680.2568320203157014No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA10300.24769380236439364No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA10280.24721284352485112No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA10010.24071989919102724No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA9240.22220298386864054No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA9070.21811483373252918No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA8780.21114093055916275No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTATGA8310.19983839782991372No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTGGA8070.1940668917554036No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGGAGAA8020.1928644946565473No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA7730.18589059148318088No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTATGA7230.17386662049461807No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTATGA7160.1721832645562193No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTCTTA7120.17122134687713425No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA7080.17025942919804923No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTTTAA7060.1697784703585067No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTATGA6940.16689271732125166No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTATGA6940.16689271732125166No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTGGGTATGA6850.16472840254331034No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTATGA6800.16352600544445406No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTATGA6760.16256408776536904No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTATGA6750.16232360834559778No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTATGA6720.16160217008628402No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTGGGTATGA6660.16015929356765649No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA6590.15847593762925768No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTGGGTATGA6330.15222347271520503No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA6210.14933771967794995No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTATGA6180.1486162814186362No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGCTTTAA6160.14813532257909368No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA6130.14741388431977992No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGCTTTAA6080.14621148722092364No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTATGA6010.14452813128252484No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGCTTTAA6010.14452813128252484No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGCTTTAA5950.1430852547638973No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA5840.1404399811464135No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA5820.139959022306871No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA5800.13947806346732847No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA5730.13779470752892967No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA5720.13755422810915843No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGCTTTAA5710.13731374868938714No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA5690.13683278984984465No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA5650.1358708721707596No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTATGA5630.1353899133312171No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA5600.13466847507190335No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA5580.13418751623236083No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGCTTTAA5580.13418751623236083No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA5540.13322559855327581No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTTA5520.1327446397137333No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTAAAA5450.1310612837753345No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA5420.13033984551602074No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTTA5360.1288969689973932No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA5350.12865648957762196No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGTAAAA5320.12793505131830818No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA5300.12745409247876566No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA5290.12721361305899442No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA5270.1267326542194519No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA5220.12553025712059562No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA5200.1250492982810531No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGAACTCA5190.12480881886128185No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTTA5160.12408738060196808No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA5130.12336594234265431No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA5110.1228849835031118No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA5100.12264450408334054No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGTAAAA5070.12192306582402679No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA5070.12192306582402679No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA5050.12144210698448427No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTTA5040.12120162756471302No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA5030.12096114814494176No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA5010.12048018930539925No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGAATGA5000.12023970988562799No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGTAAAA4950.11903731278677171No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGTAAAA4930.11855635394722919No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA4920.11831587452745794No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTAA4900.11783491568791542No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4880.11735395684837292No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA4860.1168729980088304No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGGATGA4840.1163920391692879No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTAA4840.1163920391692879No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA4830.11615155974951663No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGCTAAAA4810.11567060090997414No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC4810.11567060090997414No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTTA4770.11470868323088909No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA4750.1142277243913466No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGCTAAAA4730.11374676555180407No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGTAAAA4730.11374676555180407No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAATGA4730.11374676555180407No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTAA4710.11326580671226155No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGCTAAAA4700.1130253272924903No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA4690.11278484787271906No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGGATGA4690.11278484787271906No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTATGA4670.11230388903317653No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAATGA4660.11206340961340529No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA4650.11182293019363404No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGAATGA4630.11134197135409152No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGGATGA4620.11110149193432027No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA4610.110861012514549No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGAATGA4590.1103800536750065No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4590.1103800536750065No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA4540.10917765657615021No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC4530.10893717715637896No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCAGA4530.10893717715637896No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC4410.10605142411912388No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGTAAAA4380.10532998585981013No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGCTAAAA4360.1048490270202676No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGAATGA4350.10460854760049636No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4320.10388710934118259No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGCTAAAA4320.10388710934118259No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCAATCA4290.10316567108186882No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGCTAAAA4260.10244423282255505No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTGGGTATGA4230.10172279456324128No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCAGA4210.10124183572369876No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCAGA4200.10100135630392751No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCAGA4190.10076087688415626No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTTTAA4170.10027991804461374No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC4160.10003943862484249No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)