FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68136
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC11591.7010097452154513No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT8681.2739227427497946No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC6300.9246213455442057No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT5950.873253493013972No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4720.6927321826934366No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA4560.6692497358224727No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3670.5386286251027358No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC3250.4769872020664553No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA3080.45203710226605615No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT2830.41534577903017494No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2820.4138781261007397No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC2810.4124104731713044No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT2750.40360455559469294No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG2710.397733943876952No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC2670.391863332159211No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT2390.3507690501350241No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT2370.3478337442761536No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT2160.3170130327580134No Hit
GGGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCA2140.3140777268991429No Hit
GGGAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAA2100.30820711518140187No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG2050.30086885053422563No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2040.29940119760479045No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA1940.2847246683104379No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT1900.27885405659269696No Hit
GGGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGA1880.27591875073382643No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC1870.27445109780439125No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC1860.27298344487495596No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC1850.2715157919455207No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1820.267112833157215No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1810.2656451802277797No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG1800.2641775272983445No Hit
GGGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGC1780.261242221439474No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT1730.25390395679229777No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC1710.2509686509334273No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG1700.249500998003992No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA1700.249500998003992No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA1690.24803334507455677No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA1670.24509803921568626No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT1650.2421627333568158No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT1630.23922742749794532No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA1630.23922742749794532No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA1600.23482446870963955No Hit
GGGGGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTATCCTTTGGCTG1570.2304215099213338No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT1560.22895385699189855No Hit
GGGGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTG1540.22601855113302807No Hit
GGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAA1530.2245508982035928No Hit
GGGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCAC1530.2245508982035928No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT1510.22161559234472233No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT1510.22161559234472233No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG1470.2157449806269813No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG1470.2157449806269813No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA1460.2142773276975461No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1440.21134202183867556No Hit
GGGGGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTATGACT1430.20987436890924036No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1410.20693906305036985No Hit
GGGGACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGGACCTGAAAAA1410.20693906305036985No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA1390.20400375719149935No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA1380.20253610426206411No Hit
GGGCTGGTGACCCCAAAGCAAGGAGGACCTAGTAACATAATTGTGCTTCA1380.20253610426206411No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1330.19519783961488787No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT1320.19373018668545264No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC1320.19373018668545264No Hit
GGGGAGTTTGGTGATGCAAGAAAGGACAGTTCTCTCCACATCACTGCAGC1310.19226253375601737No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT1300.19079488082658214No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA1300.19079488082658214No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGT1280.18785957496771163No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT1260.18492426910884113No Hit
GGGATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAACGGAGTTATCT1260.18492426910884113No Hit
GGGGGAGAAAAGAAGGTTGGAATTATCGTAATTTGTTTCTAGGCTGAGAT1250.1834566161794059No Hit
GGGAGAGACACATAACACTGTGACTACCTCAGGAACCTACAAATACATCT1250.1834566161794059No Hit
GGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTTG1240.18198896324997066No Hit
GGGGGTTTTTCTGCTGTGGGTACGTGAGCAGGAAACATGGAGAAGAATCC1240.18198896324997066No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1170.17171539274392392No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC1160.17024773981448865No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCACC1150.16878008688505342No Hit
GGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACT1140.16731243395561818No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA1140.16731243395561818No Hit
GGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT1140.16731243395561818No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA1110.16290947516731244No Hit
GGGGGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAATGA1090.15997416930844194No Hit
GGGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACT1090.15997416930844194No Hit
GGGGACAGTGACCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGC1090.15997416930844194No Hit
GGGGGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT1080.1585065163790067No Hit
GGGACAGTGATCCTGATCTGGTAAAGCTCCCATCCTGCCCTGACCCTGCC1070.15703886344957144No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC1050.15410355759070093No Hit
GGGGAGGCTTTGTCGGGTGGAGCTGATTGGTTGCAGGAGCAGCAACAGTT1030.15116825173183046No Hit
GGGGAAAGAAAGGAATACCCGATGATGGAAGTAGCTCTTATGGCTGGAGA1020.14970059880239522No Hit
GGGGGGAAACCCTCTGTGCAGTGGAGTGACACAGCTGAGTACTTCTGTGC1010.14823294587295996No Hit
GGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTG1010.14823294587295996No Hit
GGGGACCACAGATCACTTGTCCCTGGAGATATAGCATCTGAGGACTGACT1000.14676529294352472No Hit
GGGAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTTTGCACCTAGA990.14529764001408949No Hit
GGGGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGC990.14529764001408949No Hit
GGGAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAG980.14382998708465422No Hit
GGGAGGAACCTACAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTT970.14236233415521896No Hit
GGGAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC970.14236233415521896No Hit
GGGACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC950.13942702829634848No Hit
GGGGAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGG950.13942702829634848No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT950.13942702829634848No Hit
GGGGAGAGGGGAAGGTGATCTCACTTGCTCAGCCTTCCCCTCCATCCTTC950.13942702829634848No Hit
GGGAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCT940.13795937536691322No Hit
GGGACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC940.13795937536691322No Hit
GGGGCACACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCTGACCGTAC930.13649172243747798No Hit
GGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTAG930.13649172243747798No Hit
GGGGAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCTA920.13502406950804274No Hit
GGGAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAA920.13502406950804274No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT910.1335564165786075No Hit
GGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCT910.1335564165786075No Hit
GGGTAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC890.130621110719737No Hit
GGGACTAGCGGGTGTAGCTCCTACGAGCAGTACTTCGGGCCGGGCACCAG890.130621110719737No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT880.12915345779030174No Hit
GGGGGTTTCAGCTGGTTGGGAAGACTGGAAGACCACCTGGGCTGTCATTG880.12915345779030174No Hit
GGGGATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGC870.1276858048608665No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG870.1276858048608665No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC870.1276858048608665No Hit
GGGGGAAAAGGACCTCAGCTTATTATAGACATTCGTTCAAATGTGGGCGA850.124750499001996No Hit
GGGATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACAAG850.124750499001996No Hit
GGGGTTGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA850.124750499001996No Hit
GGGGACTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCT850.124750499001996No Hit
GGGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTT850.124750499001996No Hit
GGGGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGGCCT830.1218151931431255No Hit
GGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG830.1218151931431255No Hit
GGGGAAACACCATATATTTTGGAGAGGGAAGTTGGCTCACTGTTGTAGAG820.12034754021369026No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA820.12034754021369026No Hit
GGGGCAGCCCCTTCCCACTGTGCTCCTACAATGAGCAGTTCTTCGGGCCA820.12034754021369026No Hit
GGGACACACATACACAGAGCCCCTACCAGGACCAGACAGCTCTTAGAGCA810.11887988728425503No Hit
GGGGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATCTTCG810.11887988728425503No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT810.11887988728425503No Hit
GGGAGACTCGCTTTTGGGAAGGGGAACCAAGTGGTGGTCATACCAAATAT810.11887988728425503No Hit
GGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTT810.11887988728425503No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG800.11741223435481977No Hit
GGGGACATTAGGCCAGGAGAAGCCCCCGAGCCAAGTCTCTTTTCTCATTC780.11447692849594927No Hit
GGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT780.11447692849594927No Hit
GGGATGGCTGGAGATTGCAGGTTTATGACTGATCCTATTTGGGAAGAACA780.11447692849594927No Hit
GGGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCC770.11300927556651404No Hit
GGGGAATCCAGGGCCCTCCTCGAGGGAAGCGGGGTTTGCGCCAGGGTCCC760.1115416226370788No Hit
GGGAGAGATACTTGATAATATAGCTCTCTTGGCTGGAGATTGCAGGTCCC760.1115416226370788No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTC760.1115416226370788No Hit
GGGGGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGAC760.1115416226370788No Hit
GGGGAAAACATTTTTGTATCATGGTGTAACATTGTGGGGACTATTCCTAC750.11007396970764353No Hit
GGGGATAGGACTGTGTGAATTATGGAGGAAGCCAAGGAAATCTCATCTTT750.11007396970764353No Hit
GGGGGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAGAGGACC750.11007396970764353No Hit
GGGGAAGAGTTCTGTGTGTAAGCAGCTTCTGGAAGGAAGGGCCCACACCA750.11007396970764353No Hit
GGGATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTA740.10860631677820828No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGG740.10860631677820828No Hit
GGGAGATGCAGTGCTTCCTGTCCCTCTGTGCCATGGGCCCCGGGCTCCTC730.10713866384877305No Hit
GGGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGC730.10713866384877305No Hit
GGGGGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTGCT720.10567101091933778No Hit
GGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGACA720.10567101091933778No Hit
GGGGACACTTACACAGTGTGACTATGGGAACAACAGACTCGCTTTTGGGA720.10567101091933778No Hit
GGGAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCC710.10420335798990255No Hit
GGGGACCAAAACAAGAGACTTGCCTAGCCCAACCTTCCTCACGCTCGCTA710.10420335798990255No Hit
GGGACTAGAGGGGAAGGTGATCTCACTTGCTCAGCCTTCCCCTCCATCCT710.10420335798990255No Hit
GGGCACAAGACTTTCTGTGATTGCAAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC710.10420335798990255No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC700.10273570506046731No Hit
GGGAGAGGCCCCATCTCAGACCCGAGGCTAGCATGGGCTGCAGGCTGCTC700.10273570506046731No Hit
GGGGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTG690.10126805213103206No Hit
GGGGAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAA690.10126805213103206No Hit
GGGATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTGGGCA690.10126805213103206No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT690.10126805213103206No Hit
GGGGATGAACTAAGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGA690.10126805213103206No Hit
GGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCAC690.10126805213103206No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)