FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953474

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953474
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences79380266
Sequences flagged as poor quality0
Sequence length20
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TAGCTGTCTCTTATACACAT1521150.19162823163127218No Hit
GGGACAAGCTCAATAACTTA1225780.15441873172861376No Hit
TAGCGCTGTCTCTTATACAC996800.12557277144926673No Hit
TAGCTCTGTCTCTTATACAC891630.11232388664457235No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTAGCTC13751150.013.994161
CTGCGCA11631750.013.9925721
TAGCGAG17220750.013.990691
GAGCGCT10514600.013.9894061
GACGTCG7555150.013.9886211
TAGCTGC8267650.013.9821
AGCGCTA10492050.013.96326352
ACGTCGA7554950.013.9615572
GCGCTAC10497250.013.9573063
TAGCTCC13738600.013.9563632
AGCTGCA8258600.013.9480362
AGCTCCT13847250.013.94545653
ACTACGC1604900.013.9431232
CGTCGAG7562950.013.9421883
CGCTACC10479100.013.9417744
AGCGAGT17386900.013.9378232
GCTGCAC8250000.013.9353663
CTACGCC1602650.013.9264733
GCGAGTA17399000.013.9220423
GCTACCT10487050.013.9188385
Produced by FastQC (version 0.11.5)