FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953475

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953475
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences26061184
Sequences flagged as poor quality0
Sequence length63
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTAGTACTCTGCGTTGATACCACTGCTTCCGCGGACAGGCGTGTAGATCTCGGTGGTCGCCGT334390.12830959637136977Illumina RNA PCR Primer (100% over 24bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATCAA316300.034.665691
TCGCCGT202950.029.23319457
GCCGTAT44750.028.52782856
CCGTATC42000.026.66415657
CGCTAGT78950.026.1599581
GTCGCCG237700.026.12232456
GGTATCA141100.025.1560361
GCTAGTA152900.025.0947532
AGCTAGT75600.022.9480971
TATCAAC516550.022.4512181
TCAACGC521900.022.0081523
CGTATCA18100.021.56882957
ATCAACG536100.021.5407492
CGTCGTA22550.020.9693222
CAACGCA547900.020.7734364
CGCCGTA64150.020.25564655
CTCGTAC70250.019.5457291
TAGTACT248750.019.3102254
GTCGTAC38550.019.2871171
ACGTCGT23350.019.2761971
Produced by FastQC (version 0.11.5)