FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953476

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953476
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10571
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN574554.346797843155805No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC9819.280105950241225No Hit
GGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT1040.9838236685271025No Hit
GGGGACAGAACGCTGTTTAAAAACTCAGTAGCATCTGGGCGTGAGCAGGC980.9270646107274618No Hit
GGGATGGGAGGCATTGTTTCCTTGTTTATGCTGAGCTCAGGGAAGAAGAA960.9081449247942485No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG890.8419260240280011No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC780.7378677513953268No Hit
GGGGGCAATCTTTACATTTCCCTCATCTTTCTTACTTCAGAGTTAGCAAA680.6432693217292592No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA640.6054299498628323No Hit
GGGAATCCCCACTCCAGAGGACCTAGGCTTAGACAAAGTGTGAACCCCAG590.5581307350297985No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC560.5297512061299783No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG530.501371677230158No Hit
GGGAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCATT510.4824519912969445No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT510.4824519912969445No Hit
GGGGGCTGCTTCCTGGGTGGAGCCCTCGGTTGCACAAATGGGTTCTGAGA500.4729921483303377No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTGACAAGCACACCCT470.44461261943051744No Hit
GGGGACCATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTT460.4351527764639107No Hit
GGGGGAAAAAAAAGCAGAATAAACAGTCTGGATGGGCAAAGCCACTCATG440.41623309053069724No Hit
GGGCTGGAGCCTCCGTAGACCTAACCATCTTCTCCTTACACCTAGCAGGT440.41623309053069724No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT430.40677324756409045No Hit
GGGATGCCTTCAATGTGATTTTCACCTTGACCCCTGTCACTGTGTGAACA430.40677324756409045No Hit
GGGGGCCATCAAATCTCAACAGAAACAGCTTTCTCTCTCTGTTATCCTGG400.37839371866427013No Hit
GGGGTGCAAAGGTAGCATAATCACTTGTTCCTTAAGTAGGGACCTGTATG380.35947403273105666No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG380.35947403273105666No Hit
GTGAGAGTGTCTATGTGAGTGTGTGTATGTGAGAGTGTGTTTGAGCCATCAGAAGCA380.35947403273105666No Hit
GGGAAGGAGGATGGAGGCAAATTTTCAACCCTGTACTCCAAAGCTGAGGG370.3500141897644499No Hit
GGGTGGTCTTAGCTCAAGCACCTATCATGAAAGCCTGGTTTTATATTACC370.3500141897644499No Hit
GGGACCTGAACCAGATAATAATTTTCTAGGTCATGCAAATATTCATGGTT360.3405543467978432No Hit
GATTCCATTCCAGTCCATTCGATGATTCCATCTGATTCCATTCAATAAAT360.3405543467978432No Hit
GGTGCTGGAGAGGATGTGGAGAAATAGGAACACTTTTGCACTGTTGGTGG340.3216346608646296No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC330.31217481789802287No Hit
GGGGATAGTCATTTGGTGCCAGTGACAGGGAAGAGCAGAGGGGCTTAGGA320.30271497493141614No Hit
GGGGTGTTTCGCCTGCACCCCTGGTTTCTTTAAGTCTTAAGTGATGCCCCTTCCAAACCATCCG320.30271497493141614No Hit
GGGGAACTGGTAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATC300.2837952889982026No Hit
GGGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGT280.26487560306498914No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA280.26487560306498914No Hit
GGGGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTATC280.26487560306498914No Hit
GGGACAATTTGGTGGGTTGGGGGAAGCCAGTGAGCCAGGAGTGCTGATTG270.25541576009838235No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA270.25541576009838235No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG270.25541576009838235No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC270.25541576009838235No Hit
GGGAAGGCCTGGGGCCCAGGAGCATGCGGAGAATCTCAGCTCCAACCTGC260.24595591713177561No Hit
GGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAA260.24595591713177561No Hit
GGGAATCTGCCCCGGAGCTGTTCCGACAGTGGCTGGAGCATTTGCTGCAC260.24595591713177561No Hit
GGGAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAAAACC260.24595591713177561No Hit
GGGTGTTCAGGCAGAGCCCTTGTGTGGAGGGCTTCAGGGGCTTCTGGCTG250.23649607416516885No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATGACAAGCACACCCTGGGAG250.23649607416516885No Hit
GGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAACA240.22703623119856212No Hit
GGGCATGGGAAATTCATGCCGGGCAGAGTGCTGGGCATGGGGAATTTGGT240.22703623119856212No Hit
GGGGCTACCATGTTATCTATAAGTTGTTTCTATCACTATAGAAATCAACA240.22703623119856212No Hit
GGGATAGTAACTTCGCCTGATAAACAACTTGATGCAGATGTTTCCCCCAAG230.21757638823195535No Hit
GGAGGTCTGGATTGTAGTGTTTGGCTCCAGGGTAATCGAGGTAATCACCA230.21757638823195535No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG220.20811654526534862No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA220.20811654526534862No Hit
GGGGAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCAT220.20811654526534862No Hit
GGGAGGCTGAGGCAGGAAGATTGTTTGAGCCATCAGAAGCA220.20811654526534862No Hit
GGGCGCGACCTCAGATCAGACGTGGCGACCCGCTGAATTTAAGCATATTA210.19865670229874183No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC210.19865670229874183No Hit
GGGCAGGGAAACTACCCTTTTTGAAACAATCAGATCTTGTGAGACTTATT200.18919685933213506No Hit
GGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTG190.17973701636552833No Hit
GGGAGAAAGACCAACGAATTGCTGTTACATTGAACAAGACAGCCAAACAT190.17973701636552833No Hit
GGGGGATCAAGACCATTATTTGGGTAACACACTAAAGATGAACTATTCTC180.1702771733989216No Hit
GGGCATTTCTTGTCAATGTAGGTGCCCTCAATAGCCTCCTTGGGTGTCTT180.1702771733989216No Hit
GGGGACTCTGTGCATACTCTGGGGCTGGGAGTTACCAACTCACTTTCGGG170.1608173304323148No Hit
GGGAAAGGCGGGCAGAGAAAGGGGAAGAAAGGAGAAAGGAGAGTTACAAG170.1608173304323148No Hit
GGGAATCAGCACAACTTTTCCCTGATTCACTCTTTTAGTGAGGCTACTTT160.15135748746570807No Hit
GGGACAGTGGCTCAGGAACCGGGGATGCAGTGCCAGGCTCATGGTATCCT160.15135748746570807No Hit
GGGGGAAAAGGCACTAAACTCTCTGTTAAACCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC160.15135748746570807No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG160.15135748746570807No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC160.15135748746570807No Hit
GGGTATTTGTCACATGGCCCTGGTGCAGGGTCCTGAGGCTTCCTCTCCCC150.1418976444991013No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCGGAAGC150.1418976444991013Illumina RNA PCR Primer (96% over 27bp)
GGGGTGTTTCGCCTGCACCCCTGGTTTCTTTAAGTCTTAAGTGATGCCCCTACCAAACCATCCG150.1418976444991013No Hit
GGGGACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCA150.1418976444991013No Hit
GGGAGCATTGTGCCGACAGAGGCTCAACCCTGGGGAGGCTATACTTTGGA150.1418976444991013No Hit
GGGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGG140.13243780153249457No Hit
GGGGGGCCTTTTTCAGTTCGGAGATCTTTATTCTTCTATTTTCTTCCCTG140.13243780153249457No Hit
GGGAAAAGTTATATAAGATTACTGAACCCAAATATTAATTATTTTTACCT140.13243780153249457No Hit
GGGGCTCTATCATTGGCTGACAAGATCCTGACAACAACACAATGAGAGCT140.13243780153249457No Hit
GGGTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG140.13243780153249457No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA130.12297795856588781No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT130.12297795856588781No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA130.12297795856588781No Hit
GGGACCAGGCTCACTGTGACAGAGGACCTGAACAAGGTGTTCCCACCCGA130.12297795856588781No Hit
GGGAGTTCTCAAAATCAAACAACAATAAGGAAAGGCTGTTGCAACCAGAA120.11351811559928106No Hit
CATCAGAAGCACTGTCTCTTATCACATCTCCGAGCCCACGAGACCCTAAG120.11351811559928106No Hit
GGGAGGAGAGTGGGCCTCAGGTGTGGTGTGGCAGAGCAGAGTAGCCACAC120.11351811559928106No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG120.11351811559928106No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT110.10405827263267431No Hit
GGGGAAAAATAAGCAAGGAGTTTCTCCAGCGAAACTTCCTCACACCTCTT110.10405827263267431No Hit
GGGGCGGAGGCGAAGAGGTGGCAGCGCGAGCTGGGACCAGCGTCTCGGAG110.10405827263267431No Hit
GGGCCTCCCTCAGCCTCTGTTTCATCTCTTCAGGAATCTCCTCTTCCCAG110.10405827263267431No Hit
GGGGATAGGACTGTGTGAATTATGGAGGAAGCCAAGGAAATCTCATCTTT110.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)