FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953477

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953477
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10571
Sequences flagged as poor quality0
Sequence length292-300
%GC43

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC4394.152871062340365No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC2091.9771071800208115No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC1881.7784504777220698No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC1681.5892536183899346No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA1661.5703339324567214No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC1651.5608740894901143No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1531.4473559738908335No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC1531.4473559738908335No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1431.352757544224766No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1231.1635606848926308No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1040.9838236685271025No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1000.9459842966606754No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC940.8892252388610349No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC900.8513858669946078No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC880.8324661810613945No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC830.7851669662283607No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC830.7851669662283607No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC820.7757071232617538No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC800.7567874373285403No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC780.7378677513953268No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC760.7189480654621133No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC750.7094882224955066No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC740.7000283795288998No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC720.6811086935956864No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA720.6811086935956864No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA710.6716488506290795No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC700.6621890076624728No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC670.6338094787626526No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC660.6243496357960457No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC640.6054299498628323No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA600.5675905779964052No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA600.5675905779964052No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA580.5486708920631918No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC510.4824519912969445No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA490.4635323053637309No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA490.4635323053637309No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC480.45407246239712423No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA480.45407246239712423No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC480.45407246239712423No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC440.41623309053069724No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA440.41623309053069724No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC410.3878535616308769No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC400.37839371866427013No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC390.3689338756976634No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA390.3689338756976634No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA370.3500141897644499No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA350.3310945038312364No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC350.3310945038312364No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA350.3310945038312364No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC340.3216346608646296No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA330.31217481789802287No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA320.30271497493141614No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA300.2837952889982026No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA300.2837952889982026No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA300.2837952889982026No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA290.2743354460315959No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA290.2743354460315959No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC290.2743354460315959No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC280.26487560306498914No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA280.26487560306498914No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC250.23649607416516885No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC250.23649607416516885No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC250.23649607416516885No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA250.23649607416516885No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC240.22703623119856212No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC230.21757638823195535No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA220.20811654526534862No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA220.20811654526534862No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA220.20811654526534862No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA210.19865670229874183No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC200.18919685933213506No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC200.18919685933213506No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC190.17973701636552833No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA190.17973701636552833No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC190.17973701636552833No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA190.17973701636552833No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC180.1702771733989216No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG180.1702771733989216No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG180.1702771733989216No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA180.1702771733989216No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC170.1608173304323148No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA160.15135748746570807No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC160.15135748746570807No Hit
CGGATTNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC150.1418976444991013No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA150.1418976444991013No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC150.1418976444991013No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA150.1418976444991013No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA150.1418976444991013No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA150.1418976444991013No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC150.1418976444991013No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA140.13243780153249457No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA140.13243780153249457No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA140.13243780153249457No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC120.11351811559928106No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA120.11351811559928106No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA120.11351811559928106No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG120.11351811559928106No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA120.11351811559928106No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA110.10405827263267431No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA110.10405827263267431No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTAACACTGCTTCCGCGGACAGGC110.10405827263267431No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA110.10405827263267431No Hit
CTTGGTGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA110.10405827263267431No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)