FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953480

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953480
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29443
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1081136.71840505383283No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC550018.680161668308255No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA10353.5152667866725538No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG6842.323132832931427No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG5061.7185748734843596No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG3741.2702509934449613No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA2830.9611792276602249No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT2690.9136297252318037No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT1790.6079543524776687No Hit
GGGGGCAGTTCGGCGGTCCCGCGGGTCTGTCTCTTGCTTCAACAGTGTTT1440.48908059640661616No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1150.39058519851917267No Hit
GGGTGTGGGCTGGTGGTGATGTAAGTCACAGTGAAGAATAAAGTCTCTCA1120.3803960194273681No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG1020.34643208912135315No Hit
GGGCTTCAAAAGTCCCCCACTATCTGCACCACTCTCTTTTCCAGCCTTAG970.3294501239683456No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAACCATCCG920.3124681588153381No Hit
GGAAGATTCCATTCCATTGCATTCCATTCCATTCCATTCCCCTGCACTCG920.3124681588153381No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG810.2751078354787216No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG790.2683150494175186No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC780.2649186563869171No Hit
GGGGGTGTGGCTAGGCTAAGCGTTTTGAGCTGCATTGCTGCGTGCTTGAT780.2649186563869171No Hit
GGGATACACCCAACACAAGAGCACTCAGATTCATAAAGTACGTTCTTAGA760.2581258703257141No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT750.2547294772951126No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTGACAAGCACACCCT740.2513330842645111No Hit
GGCAGATGTGAAGTATGAGGCAAACAAACACAAAATAAAAGACATTCACT730.2479366912339096No Hit
GGGGGACAAAAAAGGGGGAAGGGGGGGCACGAAGGCTCATCATTCAAAAT710.2411439051727066No Hit
GGGCGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAG700.23774751214210507No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT650.22076554698909756No Hit
GGGATATTCAGTCCAGGTATCAGACTCAGCCGTGTACTACTGTCTTCCGA630.21397276092789455No Hit
GGGACACCTCCCTCCTAATCCCACCAGACAACCTGCCCACCACGGGCACC580.19699079577488707No Hit
TGGATTCCATTCCGTTCCATTCTATTCGTGTTTATTTCATTCCATTCCAT580.19699079577488707No Hit
GGGGAACCTGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAA570.19359440274428558No Hit
GGGATTGTAAAGCCCCATAGGACTGTGTGAATTATGGAGGAAGCCAAGGA570.19359440274428558No Hit
GGGGACAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTT570.19359440274428558No Hit
GGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAGGTTACCCAAGGCACCC560.19019800971368406No Hit
GGGTGCAGTACACCAACATGGCACATGTATACATATGTAACAAACCTGCA550.18680161668308257No Hit
GGGATTACAGACATGAGCCACCATGTCTGGCCAAGCTCCCTAGGATTTTC550.18680161668308257No Hit
GGGAGTCTTACAATGAATAATCACATAACTCAGTTTCAGAAGTTAACCGG540.18340522365248108No Hit
AGCCCACATTGCCAAGACAATCCTAAGCCAAAAGAAGAAAGCTGGAGGCA540.18340522365248108No Hit
GGGGTTATGCTGGTCATCTCCAGAGATTCGTGCATGGTCCCAAAGAGACA520.17661243759127807No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG520.17661243759127807No Hit
GGGGGAGACAACCTGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAG520.17661243759127807No Hit
GGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCA520.17661243759127807No Hit
GGGCCAAAGTCCCAATCCATCCAGTAGTACCTAATCGCTATACCTTGCTG520.17661243759127807No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG510.17321604456067657No Hit
GGGAGGGGCTGTTGTGAAGAACTCTGACGTTTCCTGGAGACAATTTACCC510.17321604456067657No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC500.16981965153007506No Hit
GGGGCTGTCATTGAAGCAACCAGACTTAAACATTATTTGGCCACATCCTA500.16981965153007506No Hit
GGGCACATAATACAATTTGGATTAGGCACAAGACAGCAATTGGCTAAGGA500.16981965153007506No Hit
GGGACAGGAAGACTACAAAACAATAGAAAAAAAACCACTACTGTTTCTTT490.16642325849947356No Hit
GGGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATT490.16642325849947356No Hit
GGCTAACAAACCCACAGGTCCTAAACTACCAAACCATCCG480.16302686546887207No Hit
GCCCCTGGCCTGGGGTCTAACATAAACAATTAATACCAAACCATCCG480.16302686546887207No Hit
GGGGATTTCTTTTCTATCACATCAGGCTGCAAATTTTCAAAACTTTTATG470.15963047243827055No Hit
AGGCAATTACAGTGTAGGATAGTTCTGGGGATCAGAAAACAAAACCCCCA470.15963047243827055No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACTCCC460.15623407940766906No Hit
GGGTGATTCAGCCCCATATCTTCCACCTATTCATTACACGTCTCATACCT460.15623407940766906No Hit
GGGTGCAAGAGCCCAGATCACCCATTCCGGGTTCACTCCCCGCCTCCCCA450.15283768637706754No Hit
GGGGACCCAAGTGGTATTCCCATATTTATGGTGCTGGTGCCAGTGTTTGC450.15283768637706754No Hit
GGGGAGACACATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGG450.15283768637706754No Hit
GGGGGATCGCTTCCTGGTCCTCGCCTCCTCCGCTGTCTCCCTGGAGTTCT440.14944129334646605No Hit
GGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG430.14604490031586456No Hit
GGGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTTGACTGACT420.14264850728526304No Hit
GGGACGTATCTCAAAATAATAAGAGCTATTTATGACAAACCCACAGCCAATATCATACCAAACCATCCG420.14264850728526304No Hit
GGGAGCCTAGCACTCTGACCTAGCAGTCAACATGAAGGCTCTCATTGTTC420.14264850728526304No Hit
GCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG420.14264850728526304No Hit
GGGGCTAAGCAATTCTTTCGCAGTTGTATTTGTGATGAAGGAATATAAGT420.14264850728526304No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA410.13925211425466155No Hit
CGAGATTACGCCATTGCACCCCAGCCTGGGCTACAGATCAAGACTGTCTA410.13925211425466155No Hit
GGGGAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAA410.13925211425466155No Hit
GGGTGAGAGGAGGCTGGCAGAGGCTTCAATGGAGGAGGAAGATACAGAAC400.13585572122406006No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT390.13245932819345854No Hit
GGGAATCAACTGCAGTTGATTCGGTAGGAATAAGTGCCTTACACTGGCTG390.13245932819345854No Hit
GGGAACTTTTGCTTTCTACATTTCCTTCCCTTTTAATTTTTATAATTTTT390.13245932819345854No Hit
GGGAGAAAAGGAACCAAACATGTCACTTTCTAGCCTGCTGAAGGTGGTCA380.12906293516285705No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG380.12906293516285705No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA370.12566654213225556No Hit
GGGGAATTTACCTCTCCTTTTCTGCCTCACTGTTTGAGCCATCAGAAGCA370.12566654213225556No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG370.12566654213225556No Hit
GGGGGCGGGGGGTTTTTTTTTTTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG370.12566654213225556No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC360.12227014910165404No Hit
GGGGGACTCAGCAATGTATTTCTGTGCAATGAGAGAGCGAAATACTGGAG360.12227014910165404No Hit
GGGGGTACATCAAAGGATCCCAGCCTGAAGACTCAGCCACATACCTCTGT360.12227014910165404No Hit
GGGAGCCCCCCAACACACACACACACACCACACAAACACAACACACACAC350.11887375607105254No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT350.11887375607105254No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT350.11887375607105254No Hit
GGGGGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTTGGG340.11547736304045104No Hit
GGGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAA340.11547736304045104No Hit
GGGGGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTATAACCA340.11547736304045104No Hit
GGGAAAGAAAGTAGGTGTGTTAGTAATTTCTATTAGAATTAAACTAGTAG330.11208097000984954No Hit
GGGTATACGTAAGAGAAAGCCCACGTATCCATAGCTTTAACAAGGGGTCC330.11208097000984954No Hit
GGGTGGCCCAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTT330.11208097000984954No Hit
GGGCAGCAAGGGCAGCCCTGCGTCTGCGAGATATTTTCACATCAGAGACT320.10868457697924803No Hit
GGGGCTAAACCTAGCCCCAAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG320.10868457697924803No Hit
GGGACCGAAGTTCTAAAATGCATTAATGAAAAATGAAACACAACCAGAAA320.10868457697924803No Hit
GGGGGGGGAGTGGACTGGGGTGTCCCTCAGGAAGGATCCAGGGGCAGAGG320.10868457697924803No Hit
GGGGGCGTCCGGGAGGAGCCAACCGTGGCGCAGGCGGCGCGGGGAGGCGT320.10868457697924803No Hit
GGGAACAGGAGCATCATATGGCAAGAACGGTGTGAGAGAGAGAAGGGGGA320.10868457697924803No Hit
GGGGAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC310.10528818394864654No Hit
GGGGGTAGGTGAAAAGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATG310.10528818394864654No Hit
GGGGCACTTCAGAGACACCTTGATGGCCGTCTGCTCCACCCCATCCTCCC310.10528818394864654No Hit
GGGGACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCA310.10528818394864654No Hit
GGGGGAGTGGAGAAAGAGAAATTGAGCAGGTAGGGTGAGGGGGTGTATCT310.10528818394864654No Hit
GGGGCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTC310.10528818394864654No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT310.10528818394864654No Hit
GGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGG310.10528818394864654No Hit
GGGGGAAAGTGGGACTATCATGTCTGTCTTGCAGATTTCACCTCCCCAGA310.10528818394864654No Hit
GGGGGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA300.10189179091804504No Hit
GGGGCTAAACCTAGCCCCAAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG300.10189179091804504No Hit
GGGGGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTG300.10189179091804504No Hit
GGGAATATAACTGTCATGTTTATTTTCTTGGGCTGCTCTTAAGAGCTCCT300.10189179091804504No Hit
GGGAATCAAGTCCACTCGACTCCACTTCACTCCATTCCATTCCATCCCAT300.10189179091804504No Hit
GGGGAGGCCTGAACTGCCAGCCAAGAAACTGGGACCTTACCCAAGGCTGA300.10189179091804504No Hit
GGGCTGCTCTTCTTCCCCAGACAGGGTCTGTGCTTCTCCCCTAAGCCCCGGTGCCCTCATACCAAACCATCCG300.10189179091804504No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)