FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953482

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953482
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28862
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC888130.770563370521792No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN872330.22313076016908No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG10593.6691843947058413No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA6632.2971381054673965No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT3561.2334557549719354No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG2270.786501281962442No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1410.4885316332894464No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG1370.4746725798627954No Hit
GGGAAGGTGTTCCCACCCGAGGTCGCTGTGTTTGAGCCATCAGAAGCA1350.46774305314946985No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT1120.3880534959462269No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA730.25292772503638006No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG730.25292772503638006No Hit
GGGGAATAACAATGACATGCGCTTTGGAGCAGGGACCAGACTGACAGTAA680.23560390825306632No Hit
GGGAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCGTGGGGTGAC650.22520961818307808No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG650.22520961818307808No Hit
GGGAAGATGGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAA560.19402674797311345No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG550.1905619846164507No Hit
GGGAGGAATAGACGTAGACACACGAGCATATTTCACCTCCGCTACCATAA540.18709722125978798No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA510.17670293118979974No Hit
GGGAAGGCAGTGCATGCATGTTGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG500.17323816783313697No Hit
GGGGAAAGGAGAATGGAACTGTGTGTGGCGTGGTACTTGTCAGTGCTGCT490.16977340447647424No Hit
GGGAGATGGCCAGAAGCTGCTCTTTGCAAGGGGGACCATGTTAAAGGTGG480.1663086411198115No Hit
GGGCAGAATAACGGTGTAGTGGATGATTCACAGTTGCCTAAGGATCGATT480.1663086411198115No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA470.16284387776314876No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT460.15937911440648603No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT460.15937911440648603No Hit
GGGAAGATTCCATTCGAATGCATTCCATTCCATTCCATTCCCCTGCACTC430.14898482433649782No Hit
GGGAAAAAGCAGCTAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATCAC430.14898482433649782No Hit
GGGACAATAGCTAAGACCCAAACTGGGATTAGATACCCCACTATGCTTAG420.14552006097983508No Hit
GGGAGGCAGGAAGCCGGACCTCGGGGGACGCAGCAGGTCAGTGTCCCAGG420.14552006097983508No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC410.14205529762317234No Hit
GGGGGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTT410.14205529762317234No Hit
GGGAGAAAAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTG410.14205529762317234No Hit
GGGGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTT410.14205529762317234No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAACCATCCG400.1385905342665096No Hit
GGGTAAATTCGAGTCCCTTCCGTTCCACTCCTTTCCATTCGATTCCATTC400.1385905342665096No Hit
GGGAGACCCTCACCTCTCTCCCATTCTCACGTGTCCACTGGGCTCACACA380.1316610075531841No Hit
GGGTGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCA370.12819624419652137No Hit
GGGAAAATAAATGAGTTTGGAGACTTCAAATAAGATTGATGCTGAGTTTC360.12473148083985863No Hit
GGGATATATCCAAACAACAAAGCATAATATTTCGCCCACTAAGCCAATCA360.12473148083985863No Hit
TTATTTCTGTGTTCTCTATTCTGTTCCACTGATCTATGTGTCTATTTTTATACCAAACCATCCG350.1212667174831959No Hit
GGGGACCCCCATTTGTGTGACTTCATTGAGACACATTACCTGAATGAGCA350.1212667174831959No Hit
GGGATAACAAACCCTGAGAACCAAAATGAACGAAAATCTGTTCGCTTCAT340.11780195412653316No Hit
GGGAAGATTCCATTCCATTGCACTCCATTCCATTCCATTCCCCTGCACTC340.11780195412653316No Hit
GGGCACAGAAAACAGTATATTCTAGAAGTAGACCCTACTAGTAAAGAATA340.11780195412653316No Hit
GGGCGACTCTTAGCGGTGGATCACTCGGCTCGTGCGTCGATGAAGAACGC330.11433719076987042No Hit
GGGATGAATGGCTCCACGAGGGTTCAGCTGTCTCTTACTTTTAACCAGTG330.11433719076987042No Hit
GGGGAATGAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCA330.11433719076987042No Hit
GGGAATGATGGCCAGAGTCCTCAGTAAGTGCACAAAAAATAATCTCACTA330.11433719076987042No Hit
GGGGAGACACATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGG330.11433719076987042No Hit
GCTTTCCAGCTCATTCTGTGTGGCCAGTATTATCCTTATACCAAACCATCCG320.11087242741320769No Hit
GGGGGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA320.11087242741320769No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG320.11087242741320769No Hit
GGGCATTTGGAAAAAAATGTGAATCAGTCACTACTGGAACTGCACAAACT320.11087242741320769No Hit
GGGGAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCAT320.11087242741320769No Hit
GGGAGAAAATATAGGCAAACCATGCATCCAACAGGAGACTAATACCAAAA320.11087242741320769No Hit
GGGTATCTCTTTGACAAGCACACCCTGGGAGACAGTGATAATGAAAGCTA320.11087242741320769No Hit
GGGACTGACAAAAATGACCCCCATTTGTGTGACTTCATTGAGACACATTA310.10740766405654494No Hit
GGGAAATAATTACCAATAAAGTTAATAATTTAAGTAAAGATTTTAATTAT310.10740766405654494No Hit
GTGTCTTTATAGCAGCATGATTTATAATCCTTTGGGTATATACCAAGTAA310.10740766405654494No Hit
GGGGGCCCCAACGTTGTAGGCCCCTACGGGCTACTACAACCCTTCGCTGA310.10740766405654494No Hit
GGGAATATTACTTTGCATGGGGTATAATGAGGAGTGAGTGAGATAACACA300.1039429006998822No Hit
GGGAAAAGCAGCTAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATCACC300.1039429006998822No Hit
GATTCCATTCCATTGCATTCCGTTCCATTACATTCCCCTGCACTCGCGTT300.1039429006998822No Hit
GGGATAGATTCACCAGCCCTGAGCCAGGTTTGCCCACTGATGAGGAGCCT300.1039429006998822No Hit
GGGAGCGCCCGAATCTGGCTTGGCGAAATATCTCTTTGACAAGCACACCC290.10047813734321946No Hit
GGGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCAC290.10047813734321946No Hit
GGGCCAGATTCCCTTAGGATAGTGCTACCAAAACTATGAACTATAAAACT290.10047813734321946No Hit
GGAAACCGTCTGAACTATCCTGCCCGCCATCATCCTAGTCCTCATACCAAACCATCCG290.10047813734321946No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC290.10047813734321946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)