FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953483

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953483
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28862
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC11103.8458873258956414No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC5141.7808883653246483No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC4951.7150578615480563No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC4831.6734807012681034No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC4231.465594899868339No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC4191.451735846441688No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC4191.451735846441688No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC4181.4482710830850252No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC3821.3235396022451666No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA3621.254244335111912No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2720.9424156330122653No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC2350.8142193888157438No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC2290.7934308086757675No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC2290.7934308086757675No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC2230.772642228535791No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2190.7587831751091401No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC2160.7483888850391519No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC2150.7449241216824891No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC2120.734529831612501No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC2080.7206707781858499No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC2060.7137412514725244No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC2040.706811724759199No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC2030.7033469614025363No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC2020.6998821980458735No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC2010.6964174346892107No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC1980.6860231446192225No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC1960.679093617905897No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC1890.6548402744092578No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1860.6444459843392697No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1820.6305869309126186No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1800.6236574041992932No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC1720.5959392973459913No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1650.5716859538493521No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA1570.5439678469960502No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1510.5231792668560737No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG1420.4919963966461091No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1330.4608135264361444No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC1320.4573487630794817No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA1320.4573487630794817No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1200.4157716027995288No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC1170.4053773127295406No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC1150.3984477860162151No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA1140.3949830226595523No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1140.3949830226595523No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1130.39151825930288964No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG1120.3880534959462269No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1100.3811239692329014No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC1090.37765920587623864No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA1080.37419444251957595No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA1080.37419444251957595No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1070.37072967916291316No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA1060.3672649158062505No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1050.3638001524495877No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1030.3568706257362622No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC1020.3534058623795995No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA960.332617282239623No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC950.3291525188829603No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC930.32222299216963485No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC880.3048991753863211No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC860.29796964867299564No Hit
CGGATTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC770.266786778463031No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA750.25985725174970553No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC740.25639248839304274No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC740.25639248839304274No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA680.23560390825306632No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA660.22867438153974085No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG660.22867438153974085No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG650.22520961818307808No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA650.22520961818307808No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG640.22174485482641537No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA640.22174485482641537No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA610.2113505647564271No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG610.2113505647564271No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA610.2113505647564271No Hit
CGGATTNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC590.20442103804310163No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA570.19749151132977616No Hit
CGGANTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC570.19749151132977616No Hit
CGGATGGTTNNGNATAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC560.19402674797311345No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC550.1905619846164507No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA550.1905619846164507No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA540.18709722125978798No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA530.18363245790312524No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA530.18363245790312524No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG520.18016769454646248No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG520.18016769454646248No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA520.18016769454646248No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA490.16977340447647424No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG480.1663086411198115No Hit
CGGATTNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC480.1663086411198115No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA480.1663086411198115No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG440.15244958769316055No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG440.15244958769316055No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA430.14898482433649782No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA410.14205529762317234No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG410.14205529762317234No Hit
CGGANGNTTNNGNATAACTTATANAANAGGTAAANGAAACCCCAACATGC400.1385905342665096No Hit
CTTGGGGGANNCNAAAAGAACANTNGAGTNAAAAGAACGCCACTGGAGGA400.1385905342665096No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG400.1385905342665096No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA390.13512577090984684No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA370.12819624419652137No Hit
CGGATTNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC370.12819624419652137No Hit
CGGATGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC370.12819624419652137No Hit
CGGANGNTTNNGNATAACTTATANAANAGGTAAAGGAAACCCCAACATGC360.12473148083985863No Hit
CGGATGGTTNNGNTAAGGTTGTNTGGTAGNAAGGTGGAGTGGGTTTGGGG350.1212667174831959No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCTGAGAGTCCAGTTTGTATCTG340.11780195412653316No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG330.11433719076987042No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG330.11433719076987042No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG330.11433719076987042No Hit
CGGNTTGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC330.11433719076987042No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG330.11433719076987042No Hit
CGGATGGTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG320.11087242741320769No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNAAGGTGGAGTGGGTTTGGGG320.11087242741320769No Hit
CGGATGNTTNNGNATAACTTATANAANAGNTAAAGGAAACCCCAACATGC320.11087242741320769No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGTGGAGTGGGTTTGGGG310.10740766405654494No Hit
CGGATGGTTNNGNACAACTTATAGNAAAGNTAAAGGAAACCCCAACATGC310.10740766405654494No Hit
CGGATGGTTNNGNATAACTTATNNNANAGNNAAAGGAAACCCCAACATGC310.10740766405654494No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA300.1039429006998822No Hit
CGGATGGTTNNGNACAACTTATNGAAAAGNTAAAGGAAACCCCAACATGC300.1039429006998822No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG290.10047813734321946No Hit
CGGATGNTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG290.10047813734321946No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)