FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953484

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953484
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558
Sequences flagged as poor quality0
Sequence length35-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN205380.25801407349492No Hit
GGGGGATATCCATTATGTATATGTTGATATGCTTGATGGTATCCCACATG572.2283033620015638No Hit
TGATGCCCACAAAACCGCATACTCAGCCCACTCTTCTCTGCCCAATTCCC511.99374511336982No Hit
GGGATTGTAGGGTGTAAAACAAGAAACATGGAGACCAGTTTGCTGGCAAT441.7200938232994525No Hit
GGGAGGGATGGACTAAGAAGCGGAGCCTTGGCCTGCATGCTCCTCGAGGC210.8209538702111024No Hit
GGGATTATTTCCAGGACCTGTCTCATACCAAACCATCCG200.7818608287724784No Hit
GGGTGAGCTGGTGGGATTAGCTCTGCCCAGACAGGGCCTAGAAGTTGCCAAATTTAGCTCCAAACCATCCG190.7427677873338546No Hit
GGGATGAAGGGTGCAAAGGAATAGTAAAGAAAGCATGTTTGAGCCATCAGAAGCA190.7427677873338546No Hit
GGGAAATGAGATATTCCTCATCTATTACAGAGGAAAAGTAAAATCGATTG170.6645817044566067No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG130.508209538702111No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCAC130.508209538702111No Hit
GGGAGGCTCCGTGGCCAAAGTTGGGGCCGGGATCCGGAATAAGGGGCGGC100.3909304143862392No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG100.3909304143862392No Hit
GGGGGACCTGGCAGGTGGACTTGGGGAGGCAGGAGTGGAAGGCAGCAGGT100.3909304143862392No Hit
GGGAATGTTCAACTCTGTGACTTGAATGCACACATCACAAAGAAGTTTCT80.3127443315089914No Hit
GGGGTGAGTAATATTATGGTCCCCATTTCACAGCAGAGAAAACTGAGGCT80.3127443315089914No Hit
GGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAG70.2736512900703675No Hit
GGGCACCCTCACCTTTGGGAAGGGGACTATGCTTCTAGTCTCTCCAGATA70.2736512900703675No Hit
GGGGCTCTGTGAGGCATCTTGCAGCTCCCTCGACAATGCTCTGCCCGTAC50.1954652071931196No Hit
GGGAGTTCCCAAAATTGCTATGGTCTTATACACTCTGTTTCACAAGTTGT30.11727912431587179No Hit
GGGATGTGGAGGTTGCAGTGAGCTATGATTACGCCACTGCACTCCAGTCTGGGTAACAGGGTGAGAC30.11727912431587179No Hit
GGGGATGGAGTTAACAGCTACTTTGAGGTATTCCTGCGTGTAAGAGGGAC30.11727912431587179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)