FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953485

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953485
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2558
Sequences flagged as poor quality0
Sequence length294-300
%GC41

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTCG1626.333072713057075No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTCG652.541047693510555No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG572.2283033620015638No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTCG562.18921032056294No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTCG481.8764659890539486No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG451.7591868647380766No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTCG451.7591868647380766No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTCG391.5246286161063332No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG371.4464425332290853No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTCG341.3291634089132134No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTCG301.1727912431587177No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTCG291.1336982017200938No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG281.09460516028147No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG271.055512118842846No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTCG271.055512118842846No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTCG261.016419077404222No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTCG261.016419077404222No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG250.9773260359655981No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTCG250.9773260359655981No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTCG230.8991399530883503No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTCG220.8600469116497262No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG220.8600469116497262No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC210.8209538702111024No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTCG190.7427677873338546No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG180.7036747458952306No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG160.6254886630179828No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTCG160.6254886630179828No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG150.5863956215793589No Hit
CTTGNGNGANNCNTCTGCATCACNCTNCGTTGATACCACTGCTTCCGCGG140.547302580140735No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG140.547302580140735No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTCG140.547302580140735No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG140.547302580140735No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTCG140.547302580140735No Hit
CTTGGGGGANNCNTCTGCATCANNNTNCGNNGATACCACTGCTTCCGCGG130.508209538702111No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTCG130.508209538702111No Hit
CTTGGGGGANNCNTTCACTCTGNGNTNATNNCACTGCTTCCGCGGACAGG130.508209538702111No Hit
CTTGGGGGANNCNTTCACTCTGCNNTNATNCCACNGCTTCCGCGGACAGG100.3909304143862392No Hit
CGGATGGTTNNGNATCACATTGNANCTGGNAATTGGGCAGAGAAGAGTGG90.3518373729476153No Hit
CGGATGGTTNNGNATTTTGTGGNTNAACANTGACCATTTTTTAGTTTCAC90.3518373729476153No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTCG90.3518373729476153No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC90.3518373729476153No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC90.3518373729476153No Hit
CTTGGGGGANNCNTTCACTCTGNGNTNATNCCACTGCTTCCGCGGACAGG80.3127443315089914No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG80.3127443315089914No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG80.3127443315089914No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNNCACTGCTTCCGCGGACAGG70.2736512900703675No Hit
CTTGNGNGANNCNTCTGCATCACNCTNCGTTGATNCCACTGCTTCCGCGG70.2736512900703675No Hit
CTTGGGNGANNCNTCTGCATCACNCTNCGNTGATACCACTGCTTCCGCGG70.2736512900703675No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC70.2736512900703675No Hit
CTTNGGGGANNCNTTCACTCTGNNNTNATNCCACTGCTTCCGCGGACAGG70.2736512900703675No Hit
CTTGGGNGANNCNTTCACTCTGCNTTNATNCCACTGCTTCCGCGGACAGG70.2736512900703675No Hit
CTTGGGGGANNCNTCTGCATCACNNTNCGTTGATACCACTGCTTCCGCGG60.23455824863174357No Hit
CTTNGGGGANNCNTCTGCATCANNNTNCGNTGATACCACTGCTTCCGCGG60.23455824863174357No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACNGCTTCCGCGGACAGG60.23455824863174357No Hit
CTTGGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTCG60.23455824863174357No Hit
CTTGGGGGANNCNTCTGCATCACNCTNCGTTGATACCACTGCTTCCGCGG60.23455824863174357No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG60.23455824863174357No Hit
CTTGGGGGANNCNTTCACTCTGNGTTGATNCCACTGCTTCCGCGGACAGG60.23455824863174357No Hit
CTTGGGGGANNCNTTCACTCTGCGNTGATNCCACTGCTTCCGCGGACAGG60.23455824863174357No Hit
CTTNGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG60.23455824863174357No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC60.23455824863174357No Hit
CTTGGGGGANNCNTCTGCATCACNNTNCGNTGATNCCACTGCTTCCGCGG60.23455824863174357No Hit
CTTGGTGGANNCNTTCACTCTGCNNTNATACCACTGCTTCCGCGGACAGG50.1954652071931196No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC50.1954652071931196No Hit
CTTGGGNGANNCNTCTGCATCACNNTNCGTTGATACCACTGCTTCCGCGG50.1954652071931196No Hit
CTTGGGGGANNCNTTCACTCTGCNNTNATACCACTGCTTCCGCGGACAGG50.1954652071931196No Hit
CTTGNGNGANNCCTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG50.1954652071931196No Hit
CTTGGTGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTCG50.1954652071931196No Hit
CTTGGGGGANNCNTCTGCATCANTNTNCGNNGATACCACTGCTTCCGCGG50.1954652071931196No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC50.1954652071931196No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC50.1954652071931196No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC50.1954652071931196No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC50.1954652071931196No Hit
CTTNGGGGANNCNTTCACTCTGNGNTGATNNCACTGCTTCCGCGGACAGG50.1954652071931196No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNNGATACCACTGCTTCCGCGG50.1954652071931196No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC50.1954652071931196No Hit
CTTGGTGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTCG50.1954652071931196No Hit
CGGATGGTTNNGNATGAGGAAANGNAAAANAGAAGGAACAAAAATGAACA50.1954652071931196No Hit
CTTGGGGGANNCNTTCATCTGCNNNGNTANNACTGCTTCCGCGGACAGGC40.1563721657544957No Hit
CTTGNGNGANNCACTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTCG40.1563721657544957No Hit
CGGATGGTTNNGNATTTTGTGGNTNAACANNGACCATTTTTTAGTTTCAC40.1563721657544957No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC40.1563721657544957No Hit
CTTGGGGGANNCNTTCACTCTGNNNTNATNNCACTGCTTCCGCGGACAGG40.1563721657544957No Hit
CGGATGGTTNNGNATGAGGAAAANNANAAAAGAAGGAACAAAAATGAACA40.1563721657544957No Hit
CTTGGGGGANNCNTCTGCATCANTNTNCGNTGATACCACTGCTTCCGCGG40.1563721657544957No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC40.1563721657544957No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC40.1563721657544957No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC40.1563721657544957No Hit
CGGATGGTTNNGNATGAGGAAAANNANAANAGAANGAACAAAAATGAACA40.1563721657544957No Hit
CGGATGGTTNNGNATGAGGAAANNNANAANNGAAGGAACAAAAATGAACA40.1563721657544957No Hit
CTTGNGNGANNCNTTCACTCTGCNNTNATNCCACNGCTTCCGCGGACAGG40.1563721657544957No Hit
CGGANGNTTNNGNATGAGGAAAANAANAAAAGAAGGAACAAAAATGAACA40.1563721657544957No Hit
CTTGNGNGANNCNTCTGCATCACNNTNCGNTGATACCACTGCTTCCGCGG40.1563721657544957No Hit
CTTGGGNGANNCCTCTGCATCACNNTNCGTTGATACCACTGCTTCCGCGG40.1563721657544957No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG40.1563721657544957No Hit
CTTNGGGGANNCNTCTGCATCANTNTGCGNNGATACCACTGCTTCCGCGG40.1563721657544957No Hit
CTTGNGNGANNCNTTCATTCTGCNNTNATNCCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
CTTGNGNGACNCNTCTGCATCACNCTNCGTTGATACCACTGCTTCCGCGG30.11727912431587179No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC30.11727912431587179No Hit
TGCNTCTGANNGNTCAAACATGNTNTCTTNACTATTCCTTTGCACCCTTC30.11727912431587179No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT30.11727912431587179No Hit
CTTGGGNGANNCNTCTGCATCANNNTNCGTTGATNCCACTGCTTCCGCGG30.11727912431587179No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCCG30.11727912431587179No Hit
CTTGNGNGANNCNTTCACTCTGCNNTNATNCCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
CTTGGTNGANNCCTTCACTCTGCNNTNATACCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC30.11727912431587179No Hit
CTTGGTNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTCG30.11727912431587179No Hit
CGGNTGGTTNNGNTAATGAAACNNNGNGANGTCAAACATTCACACACCAA30.11727912431587179No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC30.11727912431587179No Hit
CTTGGGGGANNCNTTGCACTCTNCNTTGANACCACTGCTTCCGCGGACAG30.11727912431587179No Hit
CTTGGGGGANNCNTTCATTCTGNGNTGATNCCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCACG30.11727912431587179No Hit
CGGATGNTTNNGNATGAGACAGGNCCNGGNAATAATCCCATTCACTCTGC30.11727912431587179No Hit
CTTGNGNGACNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
CTTGGTGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTCG30.11727912431587179No Hit
CGGANGNTTNNGNGCTAAATTTGNNANCTNCTAGNCCCTGTCTGGGCAGA30.11727912431587179No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCACG30.11727912431587179No Hit
CTTNGGGGANNCNTTCACTCTGNNNTNATNNCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
TGCTTCTGANNGNTCAAACATGNTNTNTTNNCTATTCCTTTGCACCCTTC30.11727912431587179No Hit
CGGATGGTTNNGNATCACATTGGNNCNGGGAATTGGGCAGAGAAGAGTGG30.11727912431587179No Hit
TGCTNCNGANNGNTCAAACATGCNTTNTTTACTANTCCTTTGCACCCTTC30.11727912431587179No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA30.11727912431587179No Hit
CGGNTGGTTNNGNCCCCATTCANNNTNCGNTGATACCACTGCTTCCGCGG30.11727912431587179No Hit
GTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTCG30.11727912431587179No Hit
CGGATGNTTNNGTATCACATTGGNNCNGGGAATTGGGCAGAGAAGAGTGG30.11727912431587179No Hit
CGGATGNTTNNGNATGAGGAAAANAANAANAGAAGGAACAAAAATGAACA30.11727912431587179No Hit
CTTNGGGGANNCNTCTGCATCANNNTNCGNNGATACCACTGCTTCCGCGG30.11727912431587179No Hit
CTTGGTNGANNCACTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTCG30.11727912431587179No Hit
CGGNTGGTTNNGNATTTTGTGGNTNAACANNGACCATTTTTTAGTTTCAC30.11727912431587179No Hit
CGGATGGTTNNGNATCACATTGNANCNGGNNATTGGGCAGAGAAGAGTGG30.11727912431587179No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC30.11727912431587179No Hit
CTTGGAGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTCG30.11727912431587179No Hit
CTTGGTGGANNCNTCTGCATCACNCTNCGTTGATACCACTGCTTCCGCGG30.11727912431587179No Hit
CTTGGGNGANNCNTTCACTCTGCNNTNATACCACTGCTTCCGCGGACAGG30.11727912431587179No Hit
CTTGGGGGANNCNTTCACTCTGCNTTNATACCACTGCTTCCGCGGACAGG30.11727912431587179No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)