FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953486

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953486
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN415978.5161412120068No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC1863.5114215593732303No Hit
GGGATTGCATTCCATTCCTTTCCATTCTATTCCATTCCACTCCACTCCAA821.5480460638097038No Hit
GGGGCTATTCTTTCTTCAAAGCAGCCATGGGAATCAGGCTCCTCTGTCGT380.7173872003020577No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC370.6985085897677931No Hit
GGGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCC310.585236926562205No Hit
GGGAGATTTTAGTTAACAGCTATTTCTGCTTCCAAACTCTGATAACGGTT300.5663583160279403No Hit
GGGGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCATGCCCTGGC290.5474797054936756No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG280.528601094959411No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA260.49084387389088163No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG240.4530866528223523No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG220.4153294317538229No Hit
GGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGGGTTAA190.35869360015102886No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG180.3398149896167642No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT180.3398149896167642No Hit
GGGAGATAAGTGGGGTCTGGAATAGAGCTGCAGATGGGTCAGCTGCTTCA170.3209363790824995No Hit
GGGGACAGTGTCAGGGCACTGTCAGAGGAATATGAAGGCTGCAGCGATGC170.3209363790824995No Hit
GGGGGAGGCCCAAATAGCTGAAGAGGTGGAGACGTTACAGAAACCACCTG160.30205776854823485No Hit
GGGGAAATATCAGGATGGCAGTACTTGTAACCAGATTTTAATCAACATTC150.28317915801397014No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCCA150.28317915801397014No Hit
GGGAAAAAGGGACTCATCTAATCATCCAGCCCTATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC140.2643005474797055No Hit
GGGGAAATACATCTTTGGAACAGGCACCAGGCTGAAGGTTTTAGCAAATA130.24542193694544082No Hit
GGGAATCACTATGCAGAAGGACAAGGCTTCTCCTTTATCTTTGGGAAGGG130.24542193694544082No Hit
GGGGTCAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGG130.24542193694544082No Hit
GGGTCACTTTCACTCTTTCCTCTCTCCTTACTCTGATCCATTTGCTATGTGCCCTTCCAAACCATCCG120.22654332641117614No Hit
GGGGAGAGGAGAGAAGAGACAACCTGATGATAGAAGTAACTCTTATAACT120.22654332641117614No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG110.20766471587691146No Hit
GGGAGAAAATAACCCGGATATACAAAGAGCTCCTATACAACAGCCAGAAA100.18878610534264678No Hit
GGGAACTGGGAATGCAGTGCCAGGCTCGTGGTATCCTGCAGCAGATGTGG100.18878610534264678No Hit
GGGAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCC100.18878610534264678No Hit
GGGGTAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCT90.1699074948083821No Hit
GGGGGCACAGCAGGCCAGGGTGGTCTTGGAAAATGCTACATTTCGGCATG80.15102888427411743No Hit
GGGGTAAAACTAACCTGTCTCACGACGGTCTAATCCCAGCTCACGTTCCC80.15102888427411743No Hit
GGGGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGA70.13215027373985275No Hit
GGGCATCAAATTAACGAAATATCCTTGGTTAGAATACATTATGGCTAATG70.13215027373985275No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCAGAAGCA60.11327166320558807Illumina RNA PCR Primer (96% over 27bp)
GGGCGTCTCAAACAAGTTCTTCTTGTGGGAGTGGTTGTCCCAGTTGCCAG60.11327166320558807No Hit
GGGAGGAGATTCTCACACTGTACTTTCTTGATGTGGAACTTCCACCTCCC60.11327166320558807No Hit
CATCAGAAGCACTGTCTCTTATCACATCTCCGAGCCCACGAGACTCGACG60.11327166320558807No Hit
GGGCCTAAAGTAGGAGAAGACTGAAGACAAACTCTAGAACATACGTGATT60.11327166320558807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)