FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953487

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953487
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5297
Sequences flagged as poor quality0
Sequence length289-300
%GC42

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC2945.550311497073816No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1572.9639418538795543No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA1432.6996413063998492No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC1392.62412686426279No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1312.473097979988673No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC1312.473097979988673No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC1232.3220690957145553No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1212.284311874646026No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC1152.1710402114404377No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC961.812346611289409No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC731.3781385690013215No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC711.3403813479327922No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC711.3403813479327922No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC661.2459882952614687No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC661.2459882952614687No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC641.2082310741929394No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC591.1138380215216162No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA571.0760808004530866No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC571.0760808004530866No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC571.0760808004530866No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC561.057202189918822No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC561.057202189918822No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA551.0383235793845573No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC520.9816877477817633No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC510.9628091372474987No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC500.9439305267132339No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA480.9061733056447046No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA480.9061733056447046No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC480.9061733056447046No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC460.8684160845761753No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC460.8684160845761753No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA450.8495374740419105No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA340.641872758164999No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA330.6229941476307344No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA320.6041155370964697No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA300.5663583160279403No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA280.528601094959411No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA270.5097224844251463No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA270.5097224844251463No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA270.5097224844251463No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA250.4719652633566169No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC240.4530866528223523No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA240.4530866528223523No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA230.4342080422880876No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA220.4153294317538229No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA210.39645082121955827No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA200.37757221068529356No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG200.37757221068529356No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA190.35869360015102886No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC190.35869360015102886No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA190.35869360015102886No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA190.35869360015102886No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG170.3209363790824995No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA160.30205776854823485No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA160.30205776854823485No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA150.28317915801397014No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA150.28317915801397014No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA140.2643005474797055No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC140.2643005474797055No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA130.24542193694544082No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA130.24542193694544082No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT120.22654332641117614No Hit
CTTGNGNGANNCNTCTGCATCACNCTNCGTTGATNCCACTGCTTCCGCGG120.22654332641117614No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC120.22654332641117614No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC110.20766471587691146No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA100.18878610534264678No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA100.18878610534264678No Hit
CTTGGGGGANNCNTCTGCATCACNNTNCGTTGATACCACTGCTTCCGCGG90.1699074948083821No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC90.1699074948083821No Hit
CTTGGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA90.1699074948083821No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC90.1699074948083821No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC80.15102888427411743No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT80.15102888427411743No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC80.15102888427411743No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC70.13215027373985275No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA70.13215027373985275No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA70.13215027373985275No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA70.13215027373985275No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA70.13215027373985275No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA70.13215027373985275No Hit
CTTGGGGGANNCNTCTGCATCACTNTGCGNTGATACCACTGCTTCCGCGG70.13215027373985275No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA70.13215027373985275No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA70.13215027373985275No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC60.11327166320558807No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA60.11327166320558807No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC60.11327166320558807No Hit
CTTGGGGGANNCNTCTGCATCANTNTNCGNTGATACCACTGCTTCCGCGG60.11327166320558807No Hit
CTTNGGGGANNCNTCTGCATCANNNTNCGNTGATACCACTGCTTCCGCGG60.11327166320558807No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC60.11327166320558807No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA60.11327166320558807No Hit
CTTGGGGGANNCNTCTGCATCANNNTNCGNNGATACCACTGCTTCCGCGG60.11327166320558807No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA60.11327166320558807No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC60.11327166320558807No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA60.11327166320558807No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA60.11327166320558807No Hit
CGGATGGTTNNGNATGGAGTGGNGNGGAGNGGAGTGGAGTGGAATGGAAT60.11327166320558807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)