FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953489

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953489
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences124232
Sequences flagged as poor quality0
Sequence length290-300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA17331.3949706999806812No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA15201.2235172902311804No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA8030.6463713052997617No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA7460.6004894069160924No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA7290.5868053319595595No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA7210.5803657672741322No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA7170.5771459849314187No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA6910.55621739970378No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA6800.5473629982613175No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA6590.5304591409620709No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA6310.5079206645630756No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA6020.48457724257840173No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA5980.48135746023568804No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA5680.4572090926653358No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA5670.45640414707965743No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA5600.45076952797990855No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA5550.4467448000515165No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4850.39039860905402796No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC3910.3147337240002576No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3750.30185459462940306No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA3710.2986348122866894No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3710.2986348122866894No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA3650.293805138772619No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA3610.29058535642990535No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA3560.2865606285015133No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3530.28414579174447807No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA3510.2825359005731213No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA3490.28092600940176443No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA3480.28012106381608604No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA3470.2793161182304076No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA3440.2769012814733724No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA3410.2744864447163372No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA3320.2672419344452315No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA3240.26080236975980425No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3100.24953313156030654No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA3050.24550840363191448No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA3030.24389851246055766No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA3010.24228862128920087No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA3010.24228862128920087No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2980.23987378453216562No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2940.23665400218945198No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA2930.2358490566037736No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA2930.2358490566037736No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2890.23262927426105995No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA2850.22940949191834634No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA2850.22940949191834634No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2850.22940949191834634No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA2790.22457981840427588No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA2760.22216498164724066No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA2740.22055509047588384No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA2700.2173353081331702No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA2670.21492047137613496No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA2660.2141155257904566No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA2640.21250563461909974No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA2610.21009079786206453No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2600.20928585227638613No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA2580.20767596110502928No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2530.20365123317663725No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA2520.20284628759095882No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2450.19721166849121No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA2360.18996715822010432No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA2190.1762830832635714No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA2140.17225835533517933No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA2120.17064846416382254No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA1940.1561594436216112No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA1940.1561594436216112No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA1930.1553544980359328No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA1920.15454955245025437No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA1900.15293966127889755No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCAGCA1870.15052482452186233No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1830.1473050421791487No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG1780.14328031425075666No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC1740.14006053190804302No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCAATCA1690.13603580397965098No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA1610.1295962392942237No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA1600.1287912937085453No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA1580.1271814025371885No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA1540.12396162019447485No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA1530.12315667460879645No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTTA1520.12235172902311804No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG1510.12154678343743962No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGAACTCA1500.12074183785176122No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA1490.11993689226608281No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA1480.11913194668040442No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1450.11671710992336919No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA1430.11510721875201237No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTTA1430.11510721875201237No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTTA1420.11430227316633396No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA1410.11349732758065553No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC1400.11269238199497714No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC1390.11188743640929873No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA1390.11188743640929873No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA1380.11108249082362033No Hit
CGGATGGTTNNGNCTAGGGTGTNGNCTGANAATAGGGGAAATCAGTGAAT1380.11108249082362033No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGCTTCA1370.11027754523794192No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA1370.11027754523794192No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA1340.10786270848090669No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA1330.1070577628952283No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC1330.1070577628952283No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA1260.10142314379547941No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA1260.10142314379547941No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1260.10142314379547941No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCTTTAA1250.10061819820980102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)