FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953490

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953490
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12690
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN392530.929866036249017No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC12509.850275807722616No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4533.5697399527186766No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT3963.120567375886525No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT3893.065405831363278No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA3272.5768321513002364No Hit
GGGGCACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAA1541.2135539795114263No Hit
GGGGGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTATGACT1431.1268715524034674No Hit
GGGACATTAACACTATTCTCACCAGACCTCCTAGGCGACCCAGACAATTA1421.1189913317572893No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT1391.095350669818755No Hit
GGGGAGATGCCTCTGTGGACAGTTTAAGAAACCACAGTGCTTTGGAGGAA1321.0401891252955082No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC1271.000788022064618No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG1100.8668242710795903No Hit
GGGGAACTACTCCCACCCTGGAGCCTCCGTAGACCTAACCATCTTCTCCT1080.851063829787234No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA1050.8274231678486997No Hit
GGGAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGACCCTCTTGGG880.6934594168636722No Hit
GGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG860.677698975571316No Hit
GGGTTTGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAAT810.6382978723404255No Hit
GGGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAG800.6304176516942475No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT770.6067769897557131No Hit
GGGGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCACAGGAGG760.598896769109535No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC690.5437352245862884No Hit
GGGCTAAAAAATCCCAAACATATAACTGAACTCCTCACACCCAATTGGAC670.5279747832939322No Hit
GGGTTAAAGAGCAGAGTGATTGCTTTTCCCCATTTCTTATTATCAAAATT650.512214342001576No Hit
GGGAACAAGCTAACTTTTGGAGGAGGAACCAGGGTGCTAGTTAAACCAAA630.4964539007092199No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA610.4806934594168637No Hit
GGGGGTGTTTTCTAAATAGCTAAGGGATGGAGACTGTTCTGCAAGTACTC600.4728132387706856No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG580.4570527974783294No Hit
GGGAAGTTCCAAAGGTCACTGACTCCTCTTCTGCACTTTCCATTCACTTC580.4570527974783294No Hit
GGGGCTTGCTCAGCCTTCCCCTCCATCCTTCCCACCTGTTGATTATTGTA570.44917257683215134No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG560.44129235618597323No Hit
GGGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGC550.43341213553979513No Hit
GGGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCA530.4176516942474389No Hit
GGGGAACACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATAC520.4097714736012608No Hit
GGGGACCCTCTTGGGCCTGCTTATCCTTTGGCTGCAGCTGCAATGGGTGA500.3940110323089046No Hit
GGGTACCTATTCATATACCTGTTTCCCATTTTTATGTCCTCTTTTGAGAA480.37825059101654845No Hit
GGGAGACCGGAAGCGCTCCGCTGTACCTGGATCCTGCTCCTCTGGGTTGA450.3546099290780142No Hit
GGGAGTAAAGAGAGCTCATCTGTGACTGAGGAGCCTTGCTCCATTTCAGG440.3467297084318361No Hit
GGGATTGTCTTTAATTATATATCACATCATAAATTAAAAGTTCTCATAGT430.338849487785658No Hit
GGGAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGGC430.338849487785658No Hit
GGGGGAGCATGGCATGCCCTGGCTTCCTGTGGGCACTTGTGATCTCCACC430.338849487785658No Hit
GGGAAAAACTGATTCAACAATATTTAATATAGCTAAACAAAATGTCAAAATTTCTCATACCAAACCATCCG430.338849487785658No Hit
GGGTGCAAGTCTTCCATGATACTTTTTGCTCCAGCTTATTTCTGCATATT420.3309692671394799No Hit
GGGACTCTGCAGGGGAGCGCTGTCAGCATGACACGAGTTAGCTTGCTGTG420.3309692671394799No Hit
GTGCCCTTCCTAACCCCATTACTTGCCTTATTCTTCTCCACAGCACTTAT410.3230890464933018No Hit
GGGGAGGACTGAGCTTGCCTGTGACTGGCTAGGGAGGAACCTGAGACTAG400.31520882584712373No Hit
GGGGGACAACAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTT390.3073286052009456No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA390.3073286052009456No Hit
GGGATCCTTGACAATCTAAATGGTCCTATGGGTAGTTGCATAAAATATTT380.2994483845547675No Hit
GGGGGAGGATTTCGGTGAGGAGGCCGAAGAGGAGGCCTAAGGCAGAGCCC370.2915681639085894No Hit
GGGTTTTTTTTTCGTGTTTAAGGTTTGAATCCTCAGTGAACCAGGGCAGA370.2915681639085894No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT360.28368794326241137No Hit
GGGGGAGCTGAGTCTGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCT360.28368794326241137No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG350.27580772261623326No Hit
GGGGAAAATATATCTTATGGTGAAAATGCCCGGAGCAAGAAGGCAAAGCA330.26004728132387706No Hit
GGGGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG330.26004728132387706No Hit
GGGACCACAGATCACTTGTCCCTGGAGATATAGCATCTGAGGACTGACTG330.26004728132387706No Hit
GGGTTGAGAGGACCTGTGCTTAGTGTACCAATTTGGGCATGATTCAACCA320.252167060677699No Hit
GGGGGATAGGGAGCTGTGACGAGAGCAAGAGGTCAGAACACATCCAGGCT320.252167060677699No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG320.252167060677699No Hit
GGGAGCTCAGGAGCTAAATATCTAATCTGATTTTATGTGATGCTTCCCAT310.2442868400315209No Hit
GGGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTTCTGGGG310.2442868400315209No Hit
GTGGCAGAGAAGCTACCCATTACAGGACCAGGGGACACGGTTCTAGACTC310.2442868400315209No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG300.2364066193853428No Hit
GGGGCTTTTCTTTGCGGAATCACCATGGCGGCTGGGACCCTGTACACGTA290.2285263987391647No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT290.2285263987391647No Hit
GGGCTCACAGTTCCACGTGGCTGGGGTGGCCTCACAATCATGGCAGAAGG290.2285263987391647No Hit
GGGATGATTCCATTCCATTCCATTCGATGATTCCGTTTGATTCCATTCGA280.22064617809298662No Hit
GGGGGAGAGCTCCGAGAGGGCGGGATGGGCAGAGAGGACCTGAAAAACGT270.2127659574468085No Hit
GGGGATATCCTCACCGTCTTCTCAGGAACATGACTAAACCTGGGGAGGAA270.2127659574468085No Hit
GGGGCTAATCATAATACCGTCCCTTTTTGTAGGAACACCTATTGAATTTC270.2127659574468085No Hit
GGGGTTACTTCACTCAGCACAATGTCCTTTGGGTGCATGTTGCACCAGGA260.2048857368006304No Hit
GGGAAAAGACTTGGCCTTTTCCCTACTGCTCTGTAGTGCCTTCCTTGCCA260.2048857368006304No Hit
GGGAAGCAAGCACATATTCACATGGCTGAAACGAGTCAGGGGAGGTGCTA260.2048857368006304No Hit
GGGTGTGCTTCTACAAGTTAGATTTCAACATATATATGTTGATTTGGTCG260.2048857368006304No Hit
GGGATGGAAAGTCAAGCAACAGGTTTCTGTTATGAAGCATCTCACAGTGT250.1970055161544523No Hit
GGGGTAGTCTGCTGCCTCCTCCCAGACATCTGTATATTTCTGCGCCAGCA250.1970055161544523No Hit
GGGAGGGAATGTATGGAAGTTGAGATCTGTTCAGTTTCATGCAAGCAGGT250.1970055161544523No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT240.18912529550827423No Hit
GGGATTACTGTGACAATAACAATGACATGCGCTTTGGAGCAGGGACCAGA240.18912529550827423No Hit
TTCGTTCCATTCTATTCCATTGCATTACTTTCCATTCGAGTCCTTTCCAGTACATTTCATACCAAACCATCCG230.18124507486209615No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA230.18124507486209615No Hit
GGGAACTGTGTTTTCTAAATAGCTAAGGGATGGAGACTGTTCTGCAAGTA230.18124507486209615No Hit
GGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCA230.18124507486209615No Hit
GGGGCTCATTCCTGCTGTGATCCTGCCATGGATACCTGGCTCGTATGCTG220.17336485421591805No Hit
GGGAAGACATGGAAGGCAAGAGAGCTAGAAACACAAGGGGCGAAGGATGA220.17336485421591805No Hit
GGGGACATTAGGATTGGGGAAGACACTTGACTGCTAGAATCAGAGGTTGG220.17336485421591805No Hit
GGGTTGAAAAGAAAATCAACTACAGATTCAAGAATAAGGCTTACCTTCTC210.16548463356973994No Hit
GGGGAGTGCAGCTGATACTCAAGGTTCAGATCAGAAGAGGAGGCTTCTCA210.16548463356973994No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGC200.15760441292356187No Hit
GGGGAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGG190.14972419227738376No Hit
GGGTAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA180.14184397163120568No Hit
GGGAGCAACTATAAACTGACATTTGGAAAAGGAACTCTCTTAACCGTGAA180.14184397163120568No Hit
GGGTCTGTCTCCAGCATCTCAATGCCTGAGCTTCCCTGTTCAGGAGACAG170.13396375098502758No Hit
GCGGCGTAGCACAGGTACCTGATGCTGTGCATGATCTGGCCTCTACCTAT170.13396375098502758No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG170.13396375098502758No Hit
GGGGAGGAATCAGAGCCTGAGACAGACAGATGCTTCATTCCTGTATGGGG170.13396375098502758No Hit
GGGACCTGGCAGGAGAGCTGGCCATTGGCCAGGTGTTTGAGCCATCAGAAGCA160.1260835303388495No Hit
GGGAAAAAGGCATAATTAAACTTTACTTCCTCTCTTTCTTCTTCCCACTC150.1182033096926714No Hit
GGGGGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAGGAAC150.1182033096926714No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA150.1182033096926714No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA150.1182033096926714No Hit
GGGGAAGGGTACCTGAAGCGAATTGGCACCAAAGCAGCAGCTGTATTGCC150.1182033096926714No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT150.1182033096926714No Hit
GGGTCGTCGTGGTAGAAGGGCTGTTCCATTTTAGTGCACATCCGGGCGGC150.1182033096926714No Hit
GGGACAGCACCAAATCTCCACCTCCATCATCACCTCAACCCAAAAAGGCA140.11032308904649331No Hit
GGGATCACACGGTATGTGCTGTAGTGAGCCTAGCTTGATTTGCTCAACGT140.11032308904649331No Hit
GGGGGAGTGTGTGTCTGTGTGCATGTGTGTGTGCACGCACGCGCACACAG140.11032308904649331No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT140.11032308904649331No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG140.11032308904649331No Hit
GGGTGGTGACTCACTCCTGTAATCTCTGTGCTTTGGGAAGGCAATGGGAG140.11032308904649331No Hit
GGGAAGAGTTTGCTCTGCACTAGTCCGAAGGTATGTCGGGGAAGGCTTCG140.11032308904649331No Hit
GGGGATCACCTAAAAAGCTGCTACCAAGACAGCCACGAAGATCCTACCAA140.11032308904649331No Hit
GGGATTTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG130.1024428684003152No Hit
GGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTG130.1024428684003152No Hit
GGGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGA130.1024428684003152No Hit
GGGATGACACCAGTGATCCAAGTTATGGTCTATTCTGGTACAAGCAGCCC130.1024428684003152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)