FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953491

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953491
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12690
Sequences flagged as poor quality0
Sequence length288-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT3172.4980299448384558No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1931.520882584712372No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT1311.0323089046493301No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCCTT1230.9692671394799055No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCCTT1080.851063829787234No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC960.7565011820330969No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC960.7565011820330969No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT920.7249802994483846No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCCTT850.6698187549251379No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT850.6698187549251379No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC840.6619385342789598No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC820.6461780929866036No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCCTT790.6225374310480694No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC780.6146572104018913No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC750.5910165484633569No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC720.5673758865248227No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC640.504334121355398No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT640.504334121355398No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCCTT640.504334121355398No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCCTT610.4806934594168637No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT600.4728132387706856No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG580.4570527974783294No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT570.44917257683215134No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCCTT570.44917257683215134No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT560.44129235618597323No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT540.425531914893617No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT490.38613081166272656No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC480.37825059101654845No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG480.37825059101654845No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC460.3624901497241923No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC460.3624901497241923No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC440.3467297084318361No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT430.338849487785658No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT430.338849487785658No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT420.3309692671394799No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCCTT420.3309692671394799No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT420.3309692671394799No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC410.3230890464933018No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC410.3230890464933018No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCCTT400.31520882584712373No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG400.31520882584712373No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC380.2994483845547675No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTGGA370.2915681639085894No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC370.2915681639085894No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC370.2915681639085894No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC350.27580772261623326No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC350.27580772261623326No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC350.27580772261623326No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT340.26792750197005516No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC340.26792750197005516No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC340.26792750197005516No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG340.26792750197005516No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC340.26792750197005516No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC320.252167060677699No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC320.252167060677699No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT310.2442868400315209No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA300.2364066193853428No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC300.2364066193853428No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC300.2364066193853428No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT290.2285263987391647No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG290.2285263987391647No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA290.2285263987391647No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCCTT280.22064617809298662No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGCTGGA280.22064617809298662No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG270.2127659574468085No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT270.2127659574468085No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT260.2048857368006304No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA250.1970055161544523No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG250.1970055161544523No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG250.1970055161544523No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG250.1970055161544523No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG240.18912529550827423No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA240.18912529550827423No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG240.18912529550827423No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCCTT230.18124507486209615No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGCTGGA230.18124507486209615No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC230.18124507486209615No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG220.17336485421591805No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT210.16548463356973994No Hit
CGGATGNTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG210.16548463356973994No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC210.16548463356973994No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA210.16548463356973994No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCCTT200.15760441292356187No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC200.15760441292356187No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGTAAGGTGGAGTGGGTTTGGGG200.15760441292356187No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGCTGGA200.15760441292356187No Hit
CGGATGGTTNNGNATTGGGTTANGNCAGGNGGTTTTATATTGATAATTGT200.15760441292356187No Hit
CTTGGTNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT200.15760441292356187No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA200.15760441292356187No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCAATCA200.15760441292356187No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC190.14972419227738376No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC190.14972419227738376No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG190.14972419227738376No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA180.14184397163120568No Hit
CGGATGGTTNNGNATGAGGAGGNCNGCGGNTAGGAGTCAATAAAGTGATT180.14184397163120568No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG180.14184397163120568No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGCTGGA170.13396375098502758No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG170.13396375098502758No Hit
CGGATGGTTNNGNTAAGGTTGTCTNGTAGNAAGGTGGAGTGGGTTTGGGG170.13396375098502758No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG160.1260835303388495No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATTTGGCTGGA160.1260835303388495No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA160.1260835303388495No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA160.1260835303388495No Hit
CTTGGGNGANNCNTCTGCATCACNCTNCGNTGATACCACTGCTTCCGCGG160.1260835303388495No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG160.1260835303388495No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA150.1182033096926714No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA150.1182033096926714No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG150.1182033096926714No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC150.1182033096926714No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG150.1182033096926714No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA140.11032308904649331No Hit
CGGATGGTTNNGNTAAGGTTGTNTGGTAGNAAGGTGGAGTGGGTTTGGGG140.11032308904649331No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGAAAAGG140.11032308904649331No Hit
CTTGGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT140.11032308904649331No Hit
CGGANGNTTNNGNATTGGGTTATNGCNGGGGGTTTTATATTGATAATTGT140.11032308904649331No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNNAGAGTCCAGTTTGTATCTG140.11032308904649331No Hit
CTTGNGNGANNCNTTCACTCTGCNTTNATACCACNGCTTCCGCGGACAGG140.11032308904649331No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG140.11032308904649331No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC130.1024428684003152No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGCAATCA130.1024428684003152No Hit
CGGATTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC130.1024428684003152No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCCTT130.1024428684003152No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCNGAGANTCCAGTTTGTATCTG130.1024428684003152No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNAAGGTGGAGTGGGTTTGGGG130.1024428684003152No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT130.1024428684003152No Hit
CGGANTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC130.1024428684003152No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGCTGGA130.1024428684003152No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT130.1024428684003152No Hit
CTTNGGGGANNCNTTCACTCTGNGNTGATNNCACTGCTTCCGCGGACAGG130.1024428684003152No Hit
CGGANGNTTNNGCTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG130.1024428684003152No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA130.1024428684003152No Hit
CGGATGNTTNNGNATTGGGTTATNGCNGGNGGTTTTATATTGATAATTGT130.1024428684003152No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC130.1024428684003152No Hit
CTTNGGGGANNCNTCTGCATCANNNTNCGNTGATACCACTGCTTCCGCGG130.1024428684003152No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG130.1024428684003152No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGCTGGA130.1024428684003152No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC130.1024428684003152No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)