FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953502

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953502
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71635
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC14271.9920429957423047No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT10971.5313743281915264No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT7831.0930411111886646No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA7211.0064912403154884No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC5480.7649891812661408No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT5140.7175263488518183No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC5090.7105465205555943No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4430.6184127870454387No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGT4180.5835136455643191No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC3650.5095274656243457No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT3620.5053395686466112No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA3500.4885879807356739No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA3410.4760242898024709No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC3380.4718363928247365No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA3350.4676484958470022No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT3340.4662525301877574No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT3320.4634605988692678No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG3170.4425211139805961No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG3070.4285614573881482No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC2960.41320583513645565No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA2930.40901793815872123No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG2890.40343407552174215No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC2780.3880784532700496No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2540.3545752774481748No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG2510.35038738047044043No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG2340.32665596426327914No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA2270.31688420464856565No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2140.2987366510783835No Hit
GGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTGG2130.2973406854191387No Hit
GGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGG2120.2959447197598939No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA2070.28896489146367No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT2030.28338102882669086No Hit
GGGAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAA1990.27779716618971173No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT1990.27779716618971173No Hit
GGGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT1990.27779716618971173No Hit
GGGATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGA1990.27779716618971173No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA1980.27640120053046696No Hit
GGGACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCT1910.26662944091575347No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1890.26383750959726393No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT1810.2526697843233056No Hit
GGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCT1780.24848188734557128No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA1730.2415020590493474No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC1720.24010609339010258No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG1690.23591819641236825No Hit
GGGGGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAATGA1670.2331262650938787No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT1580.22056257416067565No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1510.21079081454596216No Hit
GGGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCAC1500.20939484888671736No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCAC1480.20660291756822782No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT1480.20660291756822782No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT1460.20381098624973829No Hit
GGGAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGAGG1440.2010190549312487No Hit
GGGGAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAG1440.2010190549312487No Hit
GGGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCA1370.1912472953165352No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC1350.18845536399804563No Hit
GGGAGAACTGGCAGACACCTGCCTGATGCTGCCATGGGCCCCCAGCTCCT1340.18705939833880086No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT1340.18705939833880086No Hit
GGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACT1330.1856634326795561No Hit
GGGTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAG1320.1842674670203113No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCACC1260.1758916730648426No Hit
GGGAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAAA1250.17449570740559783No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1250.17449570740559783No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG1240.17309974174635306No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT1220.17030781042786347No Hit
GGGGAGATTTGCAGCTTTCTAGGCAGGAGATAAGACAATCTGCATCTTCA1190.16611991345012914No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA1170.16332798213163957No Hit
GGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTC1160.16193201647239477No Hit
GGGGGTTTTTCTGCTGTGGGTACGTGAGCAGGAAACATGGAGAAGAATCC1150.16053605081314998No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1140.1591400851539052No Hit
GGGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGT1100.1535562225169261No Hit
GGGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGAT1090.1521602568576813No Hit
GGGATTTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG1080.1507642911984365No Hit
GGGGGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTT1080.1507642911984365No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT1080.1507642911984365No Hit
GGGGAGGACTCCTGGTTCTGGGTGCTGGGAGAGCGATGGGGCTCTCAGCG1070.14936832553919174No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG1050.14657639422070218No Hit
GGGGAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAA1040.14518042856145738No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT1030.1437844629022126No Hit
GGGGGAGCACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAG1010.14099253158372302No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC1010.14099253158372302No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT1010.14099253158372302No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT1000.13959656592447825No Hit
GGGGAGAAGTACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGT990.13820060026523348No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA990.13820060026523348No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA980.13680463460598868No Hit
GGGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCA980.13680463460598868No Hit
GGGGAGGTCAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCAT970.13540866894674392No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCTA960.13401270328749915No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT960.13401270328749915No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT960.13401270328749915No Hit
GGGAGTCATCCCTCCTCGCTGGTGAATGGAGGCAGTGGTCACAACTCTCC940.13122077196900955No Hit
GGGAGACTGCTGTCTGCACAAGAAGAAGACTTACAGATGCTTAAGGAGGA940.13122077196900955No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC940.13122077196900955No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG940.13122077196900955No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC930.12982480630976478No Hit
GGGAGAGGAAAGAAAGGAATACCCGATGATGGAAGTAGCTCTTATGGCTG920.12842884065052No Hit
GGGAATTCACCCCTCCACTTTGGGAACGGGACCAGGCTCACTGTGACAGA910.12703287499127522No Hit
GGGAAACATTAGGGCTGGGTTCATGTAAAGGGGACCAGCATTGTGCCGAC910.12703287499127522No Hit
GGGGATTTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTA910.12703287499127522No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA900.12563690933203045No Hit
GGGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCCCAC900.12563690933203045No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA900.12563690933203045No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG890.12424094367278564No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC890.12424094367278564No Hit
GGGACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCA890.12424094367278564No Hit
GGGGAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAAC880.12284497801354087No Hit
GGGGAGTTGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTA880.12284497801354087No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCC860.12005304669505129No Hit
GGGGGACAAGGCACCAGACTCACAGTTGTAGAGGACCTGAACAAGGTGTT850.11865708103580652No Hit
GGGGGACTTTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGTAG840.11726111537656173No Hit
GGGGGCCACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGG830.11586514971731696No Hit
GGGGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATCTTCG830.11586514971731696No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA820.11446918405807217No Hit
GGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG810.11307321839882738No Hit
GGGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCT810.11307321839882738No Hit
GGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCTAG800.11167725273958261No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG800.11167725273958261No Hit
GGGGGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAG790.11028128708033783No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG790.11028128708033783No Hit
GGGAAAACATTCAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAG780.10888532142109303No Hit
GGGGGAAGAGGTGGAGACGTTACAGAAACCACCTGGAGCCCCCAGAACTG770.10748935576184826No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT760.10609339010260348No Hit
GGGGGCATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCT760.10609339010260348No Hit
GGGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAA760.10609339010260348No Hit
GGGACAGACCCAGAGGACCCCTCCATCCTGCAGTTCCTGCCATGAGCCTC750.10469742444335868No Hit
GGGGGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGAGGACCTG750.10469742444335868No Hit
GGGGGGGCCGGGCACCAGGCTCACGGTCACAGAGGACCTGAAAAACGTGT740.10330145878411391No Hit
GGGGAGTTCTGTGTGTAAGCAGCTTCTGGAAGGAAGGGCCCACACCAGCT740.10330145878411391No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA740.10330145878411391No Hit
GGGGGCATTTGATTGGTTGAGCAGCCAACAGAAAGGCTCTTTCCTGCATT730.10190549312486914No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT730.10190549312486914No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG730.10190549312486914No Hit
GGGAGTGTAAATACCGGCACTGCCAGTAAACTCACCTTTGGGACTGGAAC730.10190549312486914No Hit
GGGGGGGACTCGACTCTCCATCCTAGAGGACCTGAACAAGGTGTTCCCAC720.10050952746562435No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA720.10050952746562435No Hit
GGGATTCAGCAGCTGTCTTCTCTGGGGAAGCCATTTTGTAGAGGTGTTTG720.10050952746562435No Hit
GGGATATTCCGAAATCCTCCAACAGAGACCTGTGTGAGCTTCTGCTGCAG720.10050952746562435No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)