FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953506

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953506
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50155
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN672413.406440035888744No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC49189.805602631841293No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG22384.462167281427575No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA12362.4643604825042367No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG5731.1424583790250225No Hit
GGGACTACGCTCACTGTGAAGCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC5601.1165387299371947No Hit
GGGACACACTCATGATGCACTGTGTAGCAATCAGTCCCAGCATTTTGGTG4870.9709899312132391No Hit
GGGGAATCTGAGGAAACCCTCTGTGCAGTGGAGTGACACAGCTGAGTACT3670.7317316319409829No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG3600.717774897816768No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT3340.6659355996411125No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA2780.5542817266473931No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT2710.5403249925231781No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG2540.5064300667929419No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC2530.5044362476323397No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT2490.4964609709899312No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC2340.4665536835808992No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT2130.4246834812082544No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC2060.4107267470840395No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG2050.40873292792343735No Hit
GGGGGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACAAGGTGT1970.39278237463862026No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC1920.3828132788356096No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT1780.35489981058717973No Hit
GGGGGACGCGGCTGAGTACTTCTGTGCTGTGTGTTACAGAGATGACAAGA1700.3389492573023627No Hit
GGGGATATCACAGCCTCCCAGCCTGCAGACTCAGCTAAAAGTGGAACTCA1700.3389492573023627No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC1660.33097398065995415No Hit
GGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT1480.2950852357691157No Hit
GGGAGACCAGACTAGGGCCGGGACGAGCAGTACTTCGGGCCGGGCACCAG1460.29109759744791147No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA1360.27115940584189013No Hit
GGGAAACATCCTGAGGATGTTTCCACCCGAGGTCGCTGTGTTTGAGCCATCAGAAGCA1340.2671717675206859No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT1300.25919649087827734No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA1300.25919649087827734No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT1220.24324593759346025No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA1210.24125211843285815No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG1210.24125211843285815No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG1210.24125211843285815No Hit
GGGGGAACTCTTCTGGTATGTCCAGTACTCCAGACAACGCCTCCAGTTAC1200.23925829927225603No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC1190.23726448011165388No Hit
GGGTGAATCCCAGCAGCTGCTCGATGGGCTTAAACCGCCACTCGTCAGCCTCCAGCTCTTCTACCAAACCATCCG1160.23128302262984748No Hit
GGGGAGAGGGGAAGGTGATCTCACTTGCTCAGCCTTCCCCTCCATCCTTC1130.22530156514804106No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1110.22131392682683682No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG1090.21732628850563257No Hit
GGGTTTGATTCCATTCCTTTCTATTCCATTCTTCTTGAATCCATTCCATT1070.2133386501844283No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA1050.20935101186322402No Hit
GGGAGACTTCACACACTTCAAGCCTCCCACAGCACAACTTCCCACACATA1040.20735719270262185No Hit
GGGAAGACCCTATGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAA1030.20536337354201975No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA1030.20536337354201975No Hit
GGGATGGCACATGCAGCGCAAGTAGGTCTACAAGACGCTACTTCCCCTAT1020.20336955438141757No Hit
GGGGAATCCCCACTCCAGAGGACCTAGGCTTAGACAAAGTGTGAACCCCA1010.20137573522081548No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT1000.19938191606021335No Hit
GGGAGGTTTACAGCACAGGTCGATAAATCCAGCAAGTATATCTCCTTGTT1000.19938191606021335No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA980.19539427773900908No Hit
GGGGATTACTGTGACAATAACAATGACATGCGCTTTGGAGCAGGGACCAG960.1914066394178048No Hit
GGGAGACACAGAGTCTGAGTTCTGGGGCCTGGAACCTCAATGTGCACTTG950.18941282025720269No Hit
GGGGGAGGAAAGGAAGAGATACTTGATAATATAGCTCTCTTGGCTGGAGA930.1854251819359984No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA910.18143754361479414No Hit
GGGGAGACTTCCACTAGAGGGGAAGGTGATCTCACTTGCTCAGCCTTCCC910.18143754361479414No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA900.179443724454192No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA880.17545608613298772No Hit
GGGACAAGGCTCTAGATTGGATTTTGCTTTCTTAACTCATCTCCTTCCCT840.1674808094905792No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG830.16548699032997707No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC830.16548699032997707No Hit
GGGATTAATCTATTATCAAGGTAACTCGGTCATTTTTGTCAGGCAGCACA830.16548699032997707No Hit
GGAATGTTGATTAACTTCCATTCCATTCCATTCCATCCCATCCCATTCCA830.16548699032997707No Hit
GGGCGCTCACATGCTCACTCACATACACACACACACATACACTCCCACAC810.1614993520087728No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA800.15950553284817065No Hit
GGGAAAAGGATCCCAGCCTGAAGACTCAGCCACATACCTCTGTGCCTGGG800.15950553284817065No Hit
GGGGAGGCAGACACCTGGAGGCAGGAGTGGGGAGAGGTTCCAGCTTAAAG800.15950553284817065No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGG790.15751171368756853No Hit
GGGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGG780.1555178945269664No Hit
GGGGATGACTCCATTCCATTGCATTCCGTTCCATTACATTCCCCTGCACT780.1555178945269664No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT770.15352407536636428No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG750.14953643704516No Hit
GGGTAACTTTGCAAGGAGAGCCAAAGCTAAGACCCCCGAAACCAGACGAG750.14953643704516No Hit
GGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAACA750.14953643704516No Hit
GGGGGTGAGTAAGTGTCCTCTTTGCTCCTCACAGATGTTTCCAAATCAGT750.14953643704516No Hit
GGGACATCGGCATTCTTTCCCACCCATATCATCTCCAACCCTTGTGTTTG750.14953643704516No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT750.14953643704516No Hit
GGGGGAATATGGAAACAAACTGGTCTTTGGCGCAGGAACCATTCTGAGAG740.14754261788455786No Hit
GGGAGCAGATGCCTCTGTGGACAGTTTAAGAAACCACAGTGCTTTGGAGG740.14754261788455786No Hit
GGGGATTTTTTTTGGTACAAGCAAGAATCTGGAAAAGGTCCTCAATTCAT720.1435549795633536No Hit
GGGATAGCGGGAAGGCCCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCT720.1435549795633536No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG710.14156116040275146No Hit
GGGCTTGTTGAATACGGTGAAAGATGAGATGGCTTTTAGCTGCCTCCACT710.14156116040275146No Hit
GGGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGG710.14156116040275146No Hit
GGGAACTATGGTCAGAATTTTGTCTTTGGTCCCGGAACCAGATTGTCCGT700.13956734124214934No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT700.13956734124214934No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA690.13757352208154722No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT690.13757352208154722No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGAAGATCATACCAAACCATCCG680.13557970292094507No Hit
AAACCATTTGCTCAAGAGGAAGACTCAGCCATGTATTACTGTGCTCTCCG680.13557970292094507No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG670.13358588376034294No Hit
GGCTGGGCCCGCGGCCGGCGGCTCCTCCTCCCACTCTGCTCCTCCTCTTT670.13358588376034294No Hit
GGGAACATATTTTGTCTTCCTTGTCTCCTCTCTTTCACTCACACCCTACA670.13358588376034294No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT670.13358588376034294No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA660.1315920645997408No Hit
GGGGATATGCCTTCTAATGGCAAAGATTTCTGTAGGACCCAGAGTCTTGT660.1315920645997408No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG650.12959824543913867No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT650.12959824543913867No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT650.12959824543913867No Hit
GGGGCATTTGATTGGTTGAGCAGCCAACAGAAAGGCTCTTTCCTGCATTA650.12959824543913867No Hit
GGGATGATTCCATTCAATTCCATTATATTCCGTTCAATTCCGTTGAAATC640.12760442627853655No Hit
GGGGACCCGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTAT640.12760442627853655No Hit
GGGGAAAAAAAGAACCATTTGGATACATAGGTATGGTCTGAGCTATGATA630.1256106071179344No Hit
GGGGAAGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCG630.1256106071179344No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC620.12361678795733226No Hit
GGGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATC620.12361678795733226No Hit
GGGACTCAGAACTTGTCACTCCTGCTCAAAAACTTCCAGTTTCACCCAGG610.12162296879673012No Hit
GGGAGAGGAAATGGAGAGAGATGGGAGGCAGGAAGCCGGACCTCGGGGGA610.12162296879673012No Hit
GGGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGG600.11962914963612802No Hit
GGGACTTCGGGCCAGGCACGCGGCTCCTGGTGCTCGAGGACCTGAAAAAC590.11763533047552588No Hit
GGGGATTTTCTTTTTGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCA590.11763533047552588No Hit
GGGGGGTTTCGCTTCCGCCTACCTCGCCCAGGCTGCCAGACCGGAAGCGC590.11763533047552588No Hit
GGGAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATCTTCGGGAG590.11763533047552588No Hit
GGGACACATTTGCCTCCCATTCCACCATGATTGTAATTTTCCTGAAGGTT580.11564151131492374No Hit
GGGATTCCAATCCACTCCACTCTTCTTCATTCACCTCCACTCCATTCCTT580.11564151131492374No Hit
GGGACATATTTCCTCTTCTTCTACCTTTAGCTTTTATTCCACAGCTCTTG570.1136476921543216No Hit
GGGAGAGGCAACCTCTGCTTCAATATTCCACCAAGATTTGAAATGTAAAC570.1136476921543216No Hit
GGGCAGACAGACTTGTCACCTTTGATACCACCCTTAAACAGAGTTTGTTT570.1136476921543216No Hit
GGGGTGAAAATAACCTCTTTTTTCTAATTGGTAGGACAGATTCTTTTTAT560.11165387299371947No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG560.11165387299371947No Hit
GGGAATAAAATCCACTCTGTTCAATTCCTTCCCATTCCGTTCCACTCTAC550.10966005383311733No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC550.10966005383311733No Hit
GGGGGCCCAGTAAAGGCTGGAGTCACTCAAACTCCAAGATATCTGATCAA550.10966005383311733No Hit
GGGTGTTTATATGTACTTCGGGGTGATCCCCAGCATGGTTCTCTCCTTTC550.10966005383311733No Hit
GTCCCCAGCAATCTGTAGTTTCAAATTCACCTTCATCCATACCAAACCATCCG550.10966005383311733No Hit
GGGATTCTGTAAAAAGACAGACCTTATAGGGAACAGAAGCTTACATGTAT550.10966005383311733No Hit
GGGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTT550.10966005383311733No Hit
GGGAGATATTGCAACACTGGGCCCACAGATTTTAGCAATAGCAACATTTG540.1076662346725152No Hit
GGGAGTTTCTTCTTCCTGCAGCTGGTTGAGTTCTTTCCAGACAAAGACAA540.1076662346725152No Hit
GGGAATGTCCATCTCCATGTCCTCACCTTTCTTGGCATAATTATTTGAGT540.1076662346725152No Hit
GGGGTAAGTTCCAGTCTGAATGCCTCCTGCCTGGCACTTGAGCTGAGACA540.1076662346725152No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT540.1076662346725152No Hit
GAGCAGGTGAAAGCCATCAAAGAATTGGGTGACCACGTGACCAACTTGCG540.1076662346725152No Hit
GGGTGATTTGCTATGAAGGGCAGAAAACAAAGGGAAGCAGTGTGTGTGTG540.1076662346725152No Hit
GGGGAGTACCTCTTGAGTGCAAGGTGGAGAATTAAGATCTGGATTTGAGA540.1076662346725152No Hit
GGGGTTGTGTTTCTGCCTAGGGGTGAAGAGCCAAAAGATAGAACAGAATT530.10567241551191306No Hit
GGGACCTAACCATCTTCTCCTTACACCTAGCAGGTGTCTCCTCTATCTTA530.10567241551191306No Hit
GGGCTAACTTTACTACCACTAGCTCCAGTTCAGCCCATCACCCTGCCTAA530.10567241551191306No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC530.10567241551191306No Hit
TCATGTCATCCCCCTCCACTTTCCACTTGATTCTCCCCTACTTCCTGTGT530.10567241551191306No Hit
GGGGAGACACAGAGTCTGAGTTCTGGGGCCTGGAACCTCAATGTGCACTT520.10367859635131092No Hit
GGGAAGTTATTTTAAATAAATCCCAAAGTTTGAGGCAGACTGTCCAGGAC520.10367859635131092No Hit
AGGCCCAACTGTCCAGACTAAAAATTATAAAGGAGTCACTCATGCTCACCAAACCATCCG520.10367859635131092No Hit
GGGAGGCTTTGGCAACTGACTAGTTCCCCTAATAATCGGTGCCCCCGATA510.10168477719070879No Hit
GGGATTACAGGCGTGAGCCACTGCGCCCGGCCACATTCAGTTCTTATCAA510.10168477719070879No Hit
GGGGTAGTTTTCCCCGTACATTCTTTCATTTCAAGGACTCCTGCTTTCTG510.10168477719070879No Hit
GGGAGCTTATCACGGAAAACATAGTTTTGATACTTTTTCTCATCAGCGTC510.10168477719070879No Hit
GGGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATG510.10168477719070879No Hit
GGGGAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAGG510.10168477719070879No Hit
GGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATTT510.10168477719070879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)