FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953507

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953507
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50155
Sequences flagged as poor quality0
Sequence length35-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC6051.2062605921642906No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT5421.0806499850463562No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG4130.8234473133286812No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA3360.6699232379623168No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2820.5622570032898017No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC2750.5483002691655867No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC2470.492473332668727No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC2310.4605722260990928No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT2300.45857840693849067No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC2220.44262785365367363No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA2090.4167082045658459No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCCTT2000.3987638321204267No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC1980.3947761937992224No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT1830.36486890639019043No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCCTT1810.3608812680689861No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1780.35489981058717973No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG1670.33296779982055624No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG1610.3210048848569435No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1600.3190110656963413No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCCTT1570.31302960821453496No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG1460.29109759744791147No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1450.2891037782873094No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG1440.2871099591267072No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT1430.2851161399661051No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1420.2831223208055029No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCCTT1400.2791346824842987No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA1380.27514704416309443No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG1380.27514704416309443No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA1370.2731532250024923No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA1370.2731532250024923No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG1350.269165586681288No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1310.26119031003887944No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA1270.25321503339647095No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA1270.25321503339647095No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC1250.24922739507526667No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG1220.24324593759346025No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1190.23726448011165388No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1180.23527066095105176No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1180.23527066095105176No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1170.2332768417904496No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA1170.2332768417904496No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1160.23128302262984748No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG1140.2272953843086432No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1110.22131392682683682No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA1110.22131392682683682No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA1060.21134483102382612No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC1050.20935101186322402No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1040.20735719270262185No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCCTT1030.20536337354201975No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT1020.20336955438141757No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCCTT1000.19938191606021335No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA990.1973880968996112No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT990.1973880968996112No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC970.19340045857840696No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG960.1914066394178048No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC960.1914066394178048No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA950.18941282025720269No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT920.18343136277539626No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCCTT920.18343136277539626No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC920.18343136277539626No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCCTT920.18343136277539626No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG910.18143754361479414No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA910.18143754361479414No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCCTT870.17346226697238562No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA870.17346226697238562No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT870.17346226697238562No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCCTT860.17146844781178347No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA850.16947462865118135No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC830.16548699032997707No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA820.16349317116937492No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGTGGAGTGGGTTTGGGG810.1614993520087728No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA810.1614993520087728No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA800.15950553284817065No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG790.15751171368756853No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA780.1555178945269664No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG780.1555178945269664No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA760.15153025620576213No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT740.14754261788455786No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC740.14754261788455786No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA730.14554879872395574No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA730.14554879872395574No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCCTT720.1435549795633536No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG710.14156116040275146No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCCTT710.14156116040275146No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA710.14156116040275146No Hit
CGGANGNTTTNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG700.13956734124214934No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNAAGGTGGAGTGGGTTTGGGG700.13956734124214934No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG690.13757352208154722No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCCTT690.13757352208154722No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCCTT670.13358588376034294No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC670.13358588376034294No Hit
CGGATGGTTNNGNTAAGGTTGTCTNGTAGNAAGGTGGAGTGGGTTTGGGG670.13358588376034294No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA660.1315920645997408No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGTAAGGTGGAGTGGGTTTGGGG660.1315920645997408No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA660.1315920645997408No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA650.12959824543913867No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG640.12760442627853655No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG640.12760442627853655No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA640.12760442627853655No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA640.12760442627853655No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA630.1256106071179344No Hit
CGGATGNTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG630.1256106071179344No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG630.1256106071179344No Hit
CGGATGGTTNNGNTAAGGTTGTNTGGTAGNAAGGTGGAGTGGGTTTGGGG630.1256106071179344No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG620.12361678795733226No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCCTT620.12361678795733226No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGGAGAA610.12162296879673012No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCCTT610.12162296879673012No Hit
CGGANGNTTNNGCTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG610.12162296879673012No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA610.12162296879673012No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA600.11962914963612802No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG600.11962914963612802No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA590.11763533047552588No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA590.11763533047552588No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA580.11564151131492374No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGATGA580.11564151131492374No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT580.11564151131492374No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG570.1136476921543216No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA560.11165387299371947No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA560.11165387299371947No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA560.11165387299371947No Hit
CGGATGGTTNNGNATAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC560.11165387299371947No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG550.10966005383311733No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA550.10966005383311733No Hit
CGGATGGTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG510.10168477719070879No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA510.10168477719070879No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA510.10168477719070879No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA510.10168477719070879No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)