FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953514

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953514
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50303
Sequences flagged as poor quality0
Sequence length35-300
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC7361.4631334115261514No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC4950.9840367373715285No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT4170.8289764029978333No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC4160.8269884499930422No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3990.7931932489115957No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA3270.6500606325666461No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG3150.6262051965091545No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT2360.46915690913066815No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2330.46319305011629525No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT2250.44728942607796757No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG2160.4293978490348488No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA2020.4015665069677753No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC1940.38566288292944756No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG1940.38566288292944756No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA1830.3637953998767469No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT1770.3518676818480011No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1730.3439158698288372No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT1730.3439158698288372No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC1720.3419279168240463No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG1710.3399399638192553No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1650.3280122457905095No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA1650.3280122457905095No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA1610.32006043377134563No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT1560.3101206687473908No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA1530.3041568097330179No Hit
GGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTATA1490.296204997713854No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA1490.296204997713854No Hit
GGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAA1420.28228932668031725No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA1420.28228932668031725No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA1420.28228932668031725No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT1390.2763254676659444No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC1360.27036160865157144No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCG1340.2663857026419895No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT1340.2663857026419895No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1320.2624097966324076No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA1300.2584338906228257No Hit
GGGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTGC1300.2584338906228257No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT1280.25445798461324376No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT1250.24849412559887085No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT1250.24849412559887085No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC1230.24451821958928888No Hit
GGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCACT1190.23656640757012504No Hit
GGGAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCTC1180.23457845456533408No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC1170.23259050156054312No Hit
GGGAAGAAGAAGCATGGAAGATTAATTGCCACAATAAACATACAGGAAAA1160.23060254855575216No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT1140.2266266425461702No Hit
GGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTC1120.2226507365365883No Hit
GGGAAGGAGGTGCACAGTGGGGTCAGCACAGACCCGCAGCCCCTCAAGGA1090.21668687752221535No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA1080.2146989245174244No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC1080.2146989245174244No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGG1070.21271097151263343No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC1050.20873506550305151No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC1020.2027712064886786No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT1010.20078325348388765No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGATAAGACAATCTGCATCTTCAC1000.1987953004790967No Hit
GGGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAG990.19680734747430573No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC950.18885553545514186No Hit
GGGGAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGGAGGACCTGAA950.18885553545514186No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA950.18885553545514186No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN940.18686758245035087No Hit
GGGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTT930.1848796294455599No Hit
GGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAA920.18289167644076892No Hit
GGGGACTGAACTTTGAGCTTCAGGCAGCACAACTCACATTTGTGCAAAGA910.18090372343597796No Hit
GGGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAG890.17692781742639604No Hit
GGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGACA890.17692781742639604No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC880.17493986442160508No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG870.1729519114168141No Hit
GGGGCTTTTCTCAGGGGAGAGGCCATCACTTGAAGATGCTGAGTCTTCTG870.1729519114168141No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC870.1729519114168141No Hit
GGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAG860.17096395841202314No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT840.16698805240244122No Hit
GGGGGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCAGACT830.16500009939765026No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG820.16301214639285927No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGGGTTAAAATTCTACTAAGG810.16102419338806828No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT800.15903624038327732No Hit
GGGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTT800.15903624038327732No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG800.15903624038327732No Hit
GGGATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTA790.15704828737848636No Hit
GGGGAGATTTGCAGCTTTCTAGGCAGGAGATAAGACAATCTGCATCTTCA770.15307238136890444No Hit
GGGAAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTGC770.15307238136890444No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT770.15307238136890444No Hit
GGGGATGCTCACAGAGGGCCTGGTCTAGAATATTCCACATCTGCTCTCAC760.15108442836411348No Hit
GGGATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGA750.14909647535932252No Hit
GGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCT750.14909647535932252No Hit
GGGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGAT740.14710852235453153No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGCCCTGAAGACTAAGCAAGG740.14710852235453153No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA740.14710852235453153No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT730.14512056934974057No Hit
GGGTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC720.14313261634494961No Hit
GGGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGAC720.14313261634494961No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCTA700.13915671033536767No Hit
GGGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTAT700.13915671033536767No Hit
GGGGGCTTTTGCTCACAGTGACCCTGATTGGGCAAAGCTCCCATCCTTCC700.13915671033536767No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT700.13915671033536767No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG690.1371687573305767No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA690.1371687573305767No Hit
GGGTGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCA670.13319285132099476No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT660.1312048983162038No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA650.12921694531141284No Hit
GGGGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCA650.12921694531141284No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG650.12921694531141284No Hit
GGGGGGGCAGCCCCTTCCCACTGTGCTCCTACAATGAGCAGTTCTTCGGG650.12921694531141284No Hit
GGGATGTTCACAGAGGGCCTGGTCTGGAATATTCCACATCTGCTCTCACT640.12722899230662188No Hit
GGGGGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTATGACT640.12722899230662188No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC640.12722899230662188No Hit
GGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAACA640.12722899230662188No Hit
GGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTT640.12722899230662188No Hit
GGGGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTTC630.12524103930183092No Hit
GGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCA630.12524103930183092No Hit
GGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATTT620.12325308629703995No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCG610.12126513329224896No Hit
GGGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCC610.12126513329224896No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCC610.12126513329224896No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT600.119277180287458No Hit
GGGAGTAGAGTCTGGAGAGTTTAGAATGATGGTTTTTGCAATGACTTAGA600.119277180287458No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT600.119277180287458No Hit
GGGGTAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG590.11728922728266704No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA590.11728922728266704No Hit
GGGGAAAACATCCTGAGGACAGTGCCTGGAGGGTTAAAATTCTACTAAGG590.11728922728266704No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCCA580.11530127427787608No Hit
GGGGATATTCCGAAATCCTCCAACAGAGACCTGTGTGAGCTTCTGCCGCA580.11530127427787608No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA580.11530127427787608No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGGCAGGGTTGCTGGACACTC580.11530127427787608No Hit
GGGACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGTAGC580.11530127427787608No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC570.1133133212730851No Hit
GGGAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGA560.11132536826829414No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT560.11132536826829414No Hit
GGGAGACACAGAGTCTGAGTTCTGGGGCCTGGAACCTCAATGTGCACTTG560.11132536826829414No Hit
GGGGGAGGAAACCCTCAGTCCATATAAGCGACACGGCTGAGTACTTCTGT560.11132536826829414No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG560.11132536826829414No Hit
GGGGGAAAATGATGCTGTAAAATGGAAATGAGACCTTTAGAAAGAGAGAT550.10933741526350316No Hit
GGGGACAGAAGGAATGGAGACCCTCTTGGGCCTGCTTATCCTTTGGCTGC550.10933741526350316No Hit
GGGGGGGACAGCAGAGCGGGCAGCACCGGTTTTTGTCCTGGGCCTCCAGG550.10933741526350316No Hit
GGGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTA550.10933741526350316No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGGTAACATTGTGGGGACAG550.10933741526350316No Hit
GGGAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCT540.1073494622587122No Hit
GGGGAGAGCATGTATTACTGTGACAATAACAATGACATGCGCTTTGGAGC540.1073494622587122No Hit
GGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTG540.1073494622587122No Hit
GGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCA530.10536150925392124No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA530.10536150925392124No Hit
GGGAGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCT530.10536150925392124No Hit
GGGGATGAAAAGAAGAAAGGACGAATAAGTGCCACTCTTAATACCAAGGA530.10536150925392124No Hit
GGGGGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATCTTC520.10337355624913028No Hit
GGGAGAACGAGCAGTACGTCGGGCCGGGCACCAGGCTCACGGTCACAGAG520.10337355624913028No Hit
GGGAGACATCTGTGTACTTCTGTGCCAGCAGTTCCCGGTCGACAGGGGGA520.10337355624913028No Hit
GGGAGAAACCACCTGGAGCCCCCAGAACTGGCAGACACCTGCCTGATGCT520.10337355624913028No Hit
GGGATTTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG510.1013856032443393No Hit
GGGATCAATGCACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCA510.1013856032443393No Hit
GGGAACATTGTGGGGACAGGGATCCTCCACGAGCAGTACTTCGGGCCGGG510.1013856032443393No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC510.1013856032443393No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)