FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953517

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953517
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95651
Sequences flagged as poor quality0
Sequence length290-300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA14381.5033820869619763No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA8940.9346478343143302No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA6870.7182360874428914No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA6220.6502807079905072No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA5730.5990528065571714No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA5600.5854617306666946No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA5570.5823253285381229No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA5560.5812798611619324No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA5470.5718706547762177No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA5370.5614159810143124No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGGGTGA5360.560370513638122No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA4390.4589601781476409No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4370.45686924339525986No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA3870.40459587458573354No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA3810.39832307032859043No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3800.3972776029523999No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA3730.3899593313190662No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA3580.37427732067620834No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA3340.3491861036476357No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA3280.34291329939049253No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA3080.322003951866682No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA2980.31154927810477673No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2920.3052764738476336No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA2920.3052764738476336No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA2840.29691273483810937No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2810.2937763327095378No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA2800.2927308653333473No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2770.2895944632047757No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2760.2885489958285852No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA2750.28750352845239463No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA2750.28750352845239463No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2730.2854125937000136No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA2690.28123072419525147No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA2670.27913978944287043No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA2640.27600338731429885No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCTGGGGTGA2570.26868511568096515No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA2560.26763964830477466No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA2550.2665941809285841No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCTGGGGTGA2520.26345777880001253No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2520.26345777880001253No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2510.26241231142382204No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2470.2582304419190599No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA2460.25718497454286937No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA2400.25091217028572627No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2340.24463936602858305No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA2330.24359389865239253No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA2330.24359389865239253No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCTGGGGTGA2310.2415029639000115No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA2290.23941202914763043No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA2290.23941202914763043No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2280.23836656177143992No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCTGGGGTGA2270.2373210943952494No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2250.23523015964286836No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA2190.22895735538572518No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA2160.22582095325715362No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCTGGGGTGA2140.22373001850477256No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2120.22163908375239152No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCTGGGGTGA2090.21850268162381994No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA2080.21745721424762937No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA2030.21222987736667676No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA2020.21118440999048624No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA2020.21118440999048624No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA1970.20595707310953362No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCTGGGGTGA1950.20386613835715253No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1910.19968426885239046No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1900.19863880147619994No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1880.19654786672381888No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGCTGCGT1870.19550239934762836No Hit
CGGATGGTTNNGNATGAGGCTGNCNTCCTNGTTCCACGATGAGTTGTGTT1830.19132052984286624No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA1770.18504772558572308No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1760.18400225820953256No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1760.18400225820953256No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1750.18295679083334204No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1740.18191132345715152No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCTGGGGTGA1730.180865856080961No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1720.1798203887047705No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1710.17877492132857994No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA1710.17877492132857994No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1670.17459305182381785No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1610.16832024756667469No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA1600.16727478019048417No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1580.1651838454381031No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA1570.16413837806191256No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA1560.16309291068572204No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA1550.16204744330953152No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1530.1599565085571505No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA1520.15891104118095994No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1520.15891104118095994No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA1510.1578655738047694No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA1480.15472917167619785No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATAGGGCTGGA1480.15472917167619785No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA1460.1526382369238168No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA1440.15054730217143572No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA1430.1495018347952452No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA1410.14741090004286417No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA1410.14741090004286417No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATAGGGCTGGA1410.14741090004286417No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATGGA1340.1400926284095305No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA1340.1400926284095305No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCTGGTTTTA1320.13800169365714943No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATCAGGCCTGA1320.13800169365714943No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA1320.13800169365714943No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTGGGTTTCA1310.13695622628095888No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA1290.13486529152857785No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATCTGGGGTGA1270.1327743567761968No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA1260.13172888940000627No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA1250.13068342202381575No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAAAGA1230.12859248727143469No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGGGTGA1230.12859248727143469No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTAA1220.12754701989524417No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA1220.12754701989524417No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCTGGGGTGA1220.12754701989524417No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA1220.12754701989524417No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA1210.12650155251905365No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCCGGA1190.1244106177666726No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATCAGGCCTGA1190.1244106177666726No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA1190.1244106177666726No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA1180.12336515039048207No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTGGGTTTCA1180.12336515039048207No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATCAGGCCTGA1170.12231968301429152No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA1170.12231968301429152No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATCTGGGGTGA1160.12127421563810101No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA1150.12022874826191049No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA1150.12022874826191049No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1150.12022874826191049No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCTGGGGTGA1150.12022874826191049No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCGAGCGTGA1140.11918328088571996No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA1140.11918328088571996No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATCAGGCCTGA1140.11918328088571996No Hit
GTCTCTNAGNNGGTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA1140.11918328088571996No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCTGGGGTGA1130.11813781350952944No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCGAGCGTGA1130.11813781350952944No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGAAAGA1110.11604687875714838No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCAGGCCTGA1100.11500141138095785No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTGGA1100.11500141138095785No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTGGGTTTCA1090.11395594400476733No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGGGTTCTGGATATCAGGCCTGA1090.11395594400476733No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA1090.11395594400476733No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA1080.11291047662857681No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGCTTCA1080.11291047662857681No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATCAGGCCTGA1080.11291047662857681No Hit
GTCTCTCAGNNGNTACACGGCAGGNTCAGNGTTCTGGATATCTGGGGTGA1070.11186500925238628No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCAGGCCTGA1070.11186500925238628No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGCTTCA1070.11186500925238628No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTAA1070.11186500925238628No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1050.10977407450000523No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCTGGTTTTA1050.10977407450000523No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA1050.10977407450000523No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCAGGCCTGA1040.10872860712381469No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGACTTGA1040.10872860712381469No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGAATGA1040.10872860712381469No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCGAGCGTGA1030.10768313974762417No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGAAAGA1030.10768313974762417No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCTGGGGTGA1010.10559220499524312No Hit
GTCTCTNAGNNGNTACACGGCANNNTNAGGGTTCNGGATATCTGGGGTGA1000.1045467376190526No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCTGGGGTGA1000.1045467376190526No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA1000.1045467376190526No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA990.10350127024286208No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGAAAGA980.10245580286667155No Hit
GTCTNTNAGNNGGTACACGGCAGNGTNAGGGTTCTGGATATCTGGGGTGA980.10245580286667155No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGATTCA980.10245580286667155No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTGGGTTTCA980.10245580286667155No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA960.10036486811429049No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGAATGA960.10036486811429049No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)