FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953519

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953519
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59902
Sequences flagged as poor quality0
Sequence length289-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA13552.2620279790324194No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA7351.2270041067076223No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA6531.0901138526259557No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA6121.0216687255851225No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA5760.9615705652565858No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA5550.9265133050649395No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA5480.914827551667724No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA5270.8797702914760775No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA5050.8430436379419719No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA4760.7946312310106507No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA3740.624353110079797No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA3620.6043203899702848No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA3340.557577376381423No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA3230.5392140496143701No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA3220.5375446562719108No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA3200.5342058695869921No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA3180.5308670829020734No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA3010.5024873960802644No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2980.4974792160528864No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA2960.4941404293679677No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2900.4841240693132116No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2890.48245467597075226No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA2840.47410770925845547No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA2670.44572802243664655No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2660.4440586290941872No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA2600.43404226903943105No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA2590.43237287569697175No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA2510.41901772895729694No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA2490.4156789422723782No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2400.40065440219024406No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA2300.38396046876565054No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA2250.3756135020533538No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA2210.36893592868351643No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA2210.36893592868351643No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA2210.36893592868351643No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA2090.3489032085740042No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA2000.33387866849187003No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA1880.31384594838235785No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1820.3038295883276017No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA1760.2938132282728457No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1620.2704417214784147No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACCTTGTTCA1610.2687723281359554No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1590.2654335414510367No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1570.262094754766118No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA1550.2587559680811993No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACCTTGTTCA1540.2570865747387399No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1510.2520783947113619No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACCTTGTTCA1490.2487396080264432No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACCTTGTTCA1480.24707021468398385No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA1470.2454008213415245No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACCTTGTTCA1460.2437314279990651No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1460.2437314279990651No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA1430.2387232479716871No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA1420.23705385462922773No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA1400.23371506794430905No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTTTAA1350.22536810123201229No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA1260.21034356114987812No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA1240.20700477446495943No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACCTTGTTCA1190.19865780775266267No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA1060.17695569430069114No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1040.17361690761577242No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA1040.17361690761577242No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA1030.17194751427331306No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTATGA1010.16860872758839437No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA970.16193115421855697No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA970.16193115421855697No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA890.14857600747888217No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA860.14356782745150412No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTGCA860.14356782745150412No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA850.14189843410904476No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATACGGATGAA850.14189843410904476No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA840.14022904076658543No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATACGGATGAA820.13689025408166672No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA810.13522086073920736No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCAGCA810.13522086073920736No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATACGGATGAA810.13522086073920736No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA810.13522086073920736No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTATGA800.13355146739674803No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATACGGATGAA790.13188207405428867No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA780.13021268071182932No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA770.12854328736936996No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA750.12520450068445127No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA740.12353510734199193No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATACGGATGAA740.12353510734199193No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA730.12186571399953255No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA730.12186571399953255No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA720.12019632065707322No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCAGGCCTGA720.12019632065707322No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGTTTTA710.11852692731461387No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCAGGCCTGA690.11518814062969517No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA690.11518814062969517No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA670.11184935394477646No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCTGGCTTTA670.11184935394477646No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATAGGGCTGGA660.11017996060231712No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAAAGA660.11017996060231712No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA660.11017996060231712No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATAGGGCTGGA660.11017996060231712No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTAA650.10851056725985776No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA650.10851056725985776No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATGGGGTTTGA640.10684117391739842No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGTTGA640.10684117391739842No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA630.10517178057493906No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGTAAAA620.10350238723247972No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA620.10350238723247972No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGATGA610.10183299389002036No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGTAAAA610.10183299389002036No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGCTTTAA600.10016360054756102No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)