FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004953530

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953530
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences93942
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG8050.8569117114815524No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA7930.8441378723041878No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA7650.8143322475570033No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC6680.7110770475399715No Hit
GGGGAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAAC6150.6546592578399438No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT5820.6195312001021908No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC5810.618466713504077No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG5570.5929190351493475No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA5260.5599199506078218No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG4540.4832769155436333No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT4540.4832769155436333No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT4390.4673096165719273No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT4350.4630516701794724No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT4210.4481488578058802No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA4110.4375039918247429No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA3770.4013114474888761No Hit
GGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAA3670.39066658150773886No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA3630.3864086351152839No Hit
GGGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGT3590.382150688722829No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG3450.3672478763492368No Hit
GGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGAT3440.366183389751123No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC3400.3619254433586681No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT3270.3480871175831896No Hit
GGGAGTTTTTGGATTAGGTATGTCAGCTGTGCCAGACCCCTGATCTTTGG3060.32573289902280134No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG2870.30550765365864047No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT2860.3044431670605267No Hit
GGGAGTAAGCCTCAAGACAAAAACCAGAGGAAATGGAGAGAGATGGGAGG2840.30231419386429925No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC2840.30231419386429925No Hit
GGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTG2810.29912073406995804No Hit
GGGGAGCAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCAC2640.2810244619020246No Hit
GGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAA2590.275702028911456No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA2540.27037959592088734No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG2530.26931510932277364No Hit
GGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCCT2480.26399267633220497No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC2430.25867024334163635No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA2380.2533478103510677No Hit
GGGAGTATCACTGTGTCTTATAACACCGACAAGCTCATCTTTGGGACTGG2370.2522833237529539No Hit
GGGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTT2360.2512188371548402No Hit
GGGGAAAATGATGCTGTAAAATGGAAATGAGACCTTTAGAAAGAGAGATG2350.25015435055672647No Hit
GGGGAGTATCACTGTGTCTTATAACACCGACAAGCTCATCTTTGGGACTG2350.25015435055672647No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA2310.24589640416427158No Hit
GGGAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAACT2250.23950948457558918No Hit
GGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCA2190.2331225649869068No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG2190.2331225649869068No Hit
GGGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCA2090.2224776990057695No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA2040.21715526601520088No Hit
GGGATTCACTATCATGAGAACAGCATGGGGGATGCCATCTCCATGATTCA2040.21715526601520088No Hit
GGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGAG2030.21609077941708715No Hit
GGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAG2030.21609077941708715No Hit
GGGAAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT1990.2118328330246322No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC1950.2075748866321773No Hit
GGGGAATATGAAGGCTGCAGCGATGCCTGCAAGTCAGCTTTTGCTACACT1910.20331694023972235No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT1880.2001234804453812No Hit
GGGACTACGCTCACTGTGAAGCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC1870.19905899384726747No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC1860.19799450724915374No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC1830.19480104745481255No Hit
GGGGAGTTTTTGGATTAGGTATGTCAGCTGTGCCAGACCCCTGATCTTTG1810.1926720742585851No Hit
GGGGGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGAC1810.1926720742585851No Hit
GGGAGACTGACAGGGAGCAAATAGGTAGCAAGGTTTTTCGTAATGATGCC1710.1820272082774478No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC1690.17989823508122033No Hit
GGGAGCAAGGTTTTTCGTAATGATGCCTGTGGTAGTGCTTTGACAGCACA1680.1788337484831066No Hit
GGGAGAAGCTGACTGGATATTCTGGCAGGCCAAGGATGGAGACTCTCCTG1680.1788337484831066No Hit
GGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTATA1670.17776926188499287No Hit
GGGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGC1660.17670477528687914No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT1610.17138234229631047No Hit
GGGGGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAGGAAC1590.16925336910008304No Hit
GGGGCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGG1530.16286644951140067No Hit
GGGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAG1530.16286644951140067No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC1530.16286644951140067No Hit
GGGAGTGTGATTTATAACCAGGGAGGAAAGCTTATCTTCGGACAGGGAAC1510.16073747631517318No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTC1500.15967298971705945No Hit
GGGAGAGGAGTTTATGTAAAGGGTTGGCCCAGAGTGTGTATTCAGGAGGA1490.15860850311894573No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT1490.15860850311894573No Hit
GGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTTC1470.15647952992271827No Hit
GGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCC1450.1543505567264908No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA1420.15115709693214963No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1410.1500926103340359No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA1400.14902812373592217No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA1390.14796363713780844No Hit
GGGGGTAATGATGCCTGTGGTAGTGCTTTGACAGCACAACTCTTCTTTGG1390.14796363713780844No Hit
GGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATTT1380.14689915053969468No Hit
GGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGG1370.14583466394158098No Hit
GGGGGCATTTGATTGGTTGAGCAGCCAACAGAAAGGCTCTTTCCTGCATT1360.14477017734346725No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC1360.14477017734346725No Hit
GGGAGGAAAAGATAGGCTCAGGAAAGAGAGGAAGGGTGTGCCCTCTGTCT1350.14370569074535353No Hit
GGGGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTG1340.1426412041472398No Hit
GGGGACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATG1340.1426412041472398No Hit
GGGAGAAGACCAGCCCCTTCGCCAAACAGCCTTACAAAGACATCCAGCTC1340.1426412041472398No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG1330.14157671754912604No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGGTGAGAAGGAAGCCCCCGGCC1330.14157671754912604No Hit
GGGATTTGTCCTTGAAGTATGAGGCTGGTGGCAAGAGTAACTGTGTTTCT1320.1405122309510123No Hit
GGGGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTG1310.13944774435289858No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC1310.13944774435289858No Hit
GGGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTA1310.13944774435289858No Hit
GGGAGAGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAA1310.13944774435289858No Hit
GGGGGACGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGT1300.13838325775478488No Hit
GGGGGAGAAAAGAAGGTTGGAATTATCGTAATTTGTTTCTAGGCTGAGAT1290.13731877115667115No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA1290.13731877115667115No Hit
GGGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTA1280.1362542845585574No Hit
GGGGGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGG1280.1362542845585574No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG1280.1362542845585574No Hit
GGGGGTTGAATACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAA1270.13518979796044367No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT1270.13518979796044367No Hit
GGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGGTCC1260.13412531136232994No Hit
GGGACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCTGAGGACAGTG1250.1330608247642162No Hit
GGGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGGTC1250.1330608247642162No Hit
GGGGAATGATGCCTGTGGTAGTGCTTTGACAGCACAACTCTTCTTTGGAA1240.13199633816610248No Hit
GGGGACACTCATGATGCACTGTGTAGCAATCAGCCCCAGCATTTTGGTGA1240.13199633816610248No Hit
GGGGACAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTG1230.13093185156798876No Hit
GGGAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTC1200.12773839177364757No Hit
GGGGATTCACTATCATGAGAACAGCATGGGGGATGCCATCTCCATGATTC1190.12667390517553384No Hit
GGGAGGCTGACCGTACTGGGTAAGGAGGCGGTTGGGGCTCCGGAGAGCTC1180.1256094185774201No Hit
GGGAGTCATTTGGTGCCAGTGACAGGGAAGAGCAGAGGGGCTTAGGAGGT1180.1256094185774201No Hit
GGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTC1160.12348044538119266No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGT1140.12135147218496518No Hit
GGGGACAATGAGCAGTTCTTCGGGCCAGGGACACGGCTCACCGTGCTAGA1140.12135147218496518No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC1130.12028698558685146No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCCA1110.11815801239062401No Hit
GGGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTA1110.11815801239062401No Hit
GGGGACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAA1110.11815801239062401No Hit
GGGGACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCA1100.11709352579251026No Hit
GGGGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAGGAACC1090.11602903919439654No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG1090.11602903919439654No Hit
GGGATAACACCGACAAGCTCATCTTTGGGACTGGGACCAGATTACAAGTC1080.11496455259628281No Hit
GGGGAACACAACTCATCGTGGAACCAGGAAGTCAGCCTCATACCAAACCATCCG1080.11496455259628281No Hit
GGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAACA1080.11496455259628281No Hit
GGGAGCATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAAC1080.11496455259628281No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC1080.11496455259628281No Hit
GGGACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAA1070.11390006599816908No Hit
GGGATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCCCACC1050.11177109280194161No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT1040.11070660620382788No Hit
GGGGGCACTCCTTGCAAGTCTTTACTAGTAGCTGTTGCTTCTGTTCTCTG1040.11070660620382788No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT1040.11070660620382788No Hit
GGGAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATCCT1030.10964211960571417No Hit
GGGGGTCCTCACTGTGTGCATCAGGAGGAAGCTACATACCTACATTTGGA1020.10857763300760044No Hit
GGGGAAAACAGCCTTACAAAGACATCCAGCTCTAAGGAGCTCAAAACATC1020.10857763300760044No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT1010.10751314640948671No Hit
GGGATTGATAGTCATTTGGTGCCAGTGACAGGGAAGAGCAGAGGGGCTTA1000.10644865981137297No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG1000.10644865981137297No Hit
GGGCGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC990.10538417321325924No Hit
GGGGGAGAGATGAGATCCTGGCCTGGACCTGAAATGGGCACAAGGTTGTT990.10538417321325924No Hit
GGGGATTTTGGTGATGGGACTCGACTCTCCATCCTAGAGGACCTGAACAA980.10431968661514551No Hit
GGGGAAAGAGAGCTCATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTC980.10431968661514551No Hit
GGGGGAGTTTGACGCTGTGTGGAATATGGAAACAAACTGGTCTTTGGCGC980.10431968661514551No Hit
GGGGCTTCTTGGCTCATCCTCCCTCTTATGTGCATGGCTCTGCCTCTCCT980.10431968661514551No Hit
GGGAGGCAGGAAGCCGGACCTCGGGGGACGCAGCAGATATCCAGAACCCT970.1032552000170318No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA970.1032552000170318No Hit
GGGGAGACTGACAGGGAGCAAATAGGTAGCAAGGTTTTTCGTAATGATGC970.1032552000170318No Hit
GGGGAAGGTCCTGTCCTCCTGGTGACAGTAGTTACGGGTGGAGAAGTGAA970.1032552000170318No Hit
GGGGGGGACTGGAACAAGACTTCAGGTCACGCTCGATATCCAGAACCCTG960.10219071341891804No Hit
GGGGGACCTTTAGAAAGAGAGATGCTGAACATGAGAAATCAGTAAGCCTC940.1000617402226906No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT940.1000617402226906No Hit
GGGACTGTGTAGCAATCAGCCCCAGCATTTTGGTGATGGGACTCGACTCT940.1000617402226906No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)