FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004953532

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953532
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48668047
Sequences flagged as poor quality0
Sequence length21
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGG992100.20385038257236826No Hit
GCAGCGTAAACCGGGGGGGGT555660.11417347402495934No Hit

[WARN]Adapter Content

Can't analyse adapters as read length is too short

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTGATC5445200.014.9874211
TACTACG4212250.014.98051551
CTGCGCA5192450.014.9789151
CGCTCAG4429500.014.9747911
TCTTAGG8304050.014.9725092
GTCTTAG8276450.014.97215751
TAGCTCC2623350.014.9715862
ACTACGC4266650.014.968142
CAGCGTA7722800.014.9644492
GCAGCGT7650400.014.9626661
CTGATCG5462300.014.9606092
GTAGCTC2608350.014.9573111
AGCGTAA7732100.014.9507433
AGCGCTA2818900.014.9490542
CTACGCC4271550.014.9456483
AGCTCCT2635350.014.9433783
TTAGGAA8299850.014.9423484
CTTAGGA8320950.014.9418623
AGGCTCC5200850.014.9391461
ATCGTTG5471000.014.9381155
Produced by FastQC (version 0.11.5)