FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953534

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953534
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535
Sequences flagged as poor quality0
Sequence length35-300
%GC47

[WARN]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN157613.662765496315563No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC10849.397485912440398No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA6055.244906805374946No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG5985.18422193324664No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG2512.175986129172085No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA2251.950585175552666No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1571.3610749891634157No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT1351.1703511053315996No Hit
GGGAGGAGGAAACAAACTCACCTTTGGGACAGGCACTCAGCTAAAAGTGG1140.988296488946684No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1100.953619419159081No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA1010.8755960121369745No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG950.8235804074555699No Hit
GGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTA580.5028175119202427No Hit
GGGGAGACTCAGCTACCTACCTCTGTGCTCTAGACATAATAGAGCGGCAC580.5028175119202427No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCGGAAGC460.3987863025574339Illumina RNA PCR Primer (96% over 27bp)
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT460.3987863025574339No Hit
GGGGAGAGCGATGGGGCTCTCAGCGGTGGGAAGGACCCGAGCTGAGTCTG390.3381014304291287No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT330.2860858257477243No Hit
GGGAAAAACAAACACACACACACATCTTAAAACAATAACTCCTTATAACC330.2860858257477243No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA330.2860858257477243No Hit
GGGATAACCATGCACACTACTATAACCACCCTAACCCTGACTTCCCTAAT330.2860858257477243No Hit
GGGATCTAATTAAACTAAAGAGCTTCTGCACAGCAAAAGAAACTACCATC320.27741655830082357No Hit
GGGATTCCCCAGAATCTCTTAACTCGGCTTACACGCTAAACTCAAGACAG320.27741655830082357No Hit
ACCCCATTCTCCATGATGTTCTTATTTCATATTGCATGCTTGTATTATACCAAACCATCCG310.26874729085392285No Hit
GGGATTAATCCCCTGGCCCAACCCGTCATCTACTCTACCATCTTTGCAGG310.26874729085392285No Hit
AGACCACACCACACACACCACCCATCACACACATACACACCACACACATA290.25140875596012136No Hit
GGGCGCTTGCAGACACACACATTCACACACACACCACATACCACACACAC280.24273948851322064No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG270.23407022106631986No Hit
GGGAGAAAGTTCCCTTGTCTAGTATCTTCTATGTCACTCAACATTTCTGT270.23407022106631986No Hit
GGGATTGATTCTATTCAATTCCCTTACACTCCATTACATTCCATTCCATT260.22540095361941914No Hit
GGGTCACGGCGGCCTTTTTTTTTTTAGTTTAACTTTTCCTTTTTTGCTGT250.21673168617251842No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG250.21673168617251842No Hit
GGGGCCCCAGCGCTGGCTGTCTGCTACAGAACCTCAGTTCTCATCTTCTG240.20806241872561768No Hit
GGGAGGAAGAAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAA230.19939315127871696No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT230.19939315127871696No Hit
CCTGAGAGTCATCCATGACAGCTCCCTCTCCTCTCTGTTATCCCCGCATC220.1907238838318162No Hit
GGGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGAAG220.1907238838318162No Hit
GGGGGCTTTTATATGAAGTCGGGGCAGTGGGAGCAGAAAAGAGGGCTGAG220.1907238838318162No Hit
GGGTTTAAAGGCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT220.1907238838318162No Hit
GGGTATTGATTGTATTCAATTCCCTTACACTCCATTACATTCCATTCCAT220.1907238838318162No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA210.18205461638491546No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTT210.18205461638491546No Hit
GGGAATAGAAACACAAAAAACCCTTCAAAAATCAGTGAATCCAGGGGCTA210.18205461638491546No Hit
GGGGAATGAGACAGCCAGATGGGAGGGGGATACTTGGAGAAACTCCAACC200.17338534893801474No Hit
GGGACAATTTGGTGGGTTGGGGGAAGCCAGTGAGCCAGGAGTGCTGATTG200.17338534893801474No Hit
GGGGAGGAGGCTACTCAGTAGACAGTCCCACCCTCACACGATTCTTTACC200.17338534893801474No Hit
GGGGGCTTTTTTTGGTTGGTAGGCTTTTTATTACTGATTCAATTGTGGAA200.17338534893801474No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC200.17338534893801474No Hit
GGGATGGTATAATACGCCTCACACTCATTCTCAACCCCCTGACAAAACAC200.17338534893801474No Hit
GGGCATCTACCATCACCCTCTACATCACCGCCCCGACCTTAGCTCTCACC200.17338534893801474No Hit
GGGCATGGAAGATCACTCAGTGTTATGGGAACTGTGAAATCAAGGAACTG190.164716081491114No Hit
GGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGT190.164716081491114No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG190.164716081491114No Hit
GGGAAAAATGTCACATTCAAAATGATGATAGAGGCCAGACGTGGTGGCGC190.164716081491114No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG190.164716081491114No Hit
GGGCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG190.164716081491114No Hit
GGGGGAGCGGTGAACACCGGGGAGCTGTTTTTTGGAGAAGGCTCTAGGCT180.15604681404421328No Hit
GGGAGAAAATTTTTGCAAACTGTACATCTGACAGAGGTCTAATATCCAGC180.15604681404421328No Hit
GGGGTAGCCCACTTCCACTATGTCCTATCAATAGGAGCTGTATTTGCCAT180.15604681404421328No Hit
GGGCAGAGTGTGGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTT180.15604681404421328No Hit
GGGACATACCCATGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATC170.14737754659731253No Hit
GGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGCC170.14737754659731253No Hit
GGGAAGATCTAAAATTGACACGCTAACATCACAATTAAAAGAACTAGAGA170.14737754659731253No Hit
GGGTTAATTCCATTCCGTTCCTTTCCATTCCATTCCATTCTGTTCCATTC170.14737754659731253No Hit
GGGGGCCATCAAATCTCAACAGAAACAGCTTTCTCTCTCTGTTATCCTGG170.14737754659731253No Hit
GGGCTAACCACCTCTAACCACTACTAACCACCCACCTAACCATCCACCTA160.13870827915041178No Hit
GGGAGGAAGAAAGGAAAGTGAAGAAGCCATCCTAGCAGATGATGATGAAC160.13870827915041178No Hit
GGGATAATCGGTGCCCCCGATATGGCGTTTCCCCGCATAAACAACATAAG160.13870827915041178No Hit
GGGGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAACTA160.13870827915041178No Hit
GGCAGACTTGTACATGTCATCCAGGACCTTCCACCCATCCATCTGCACAT160.13870827915041178No Hit
GGGGAGGCTTAGTATCTCTGATACTCTTACTGCTTGGAAGAACCCGTGGA160.13870827915041178No Hit
GGGACAAAACACATAGCCTACCCCTTCCTTGTACTATCCCTATGAGGCAT160.13870827915041178No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAACCATCCG150.13003901170351106No Hit
GGGGAGGGGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAG150.13003901170351106No Hit
GGGCACACCTACACCCCTTATCCCCATACTAGTTATTATCGAAACCATCA150.13003901170351106No Hit
GGGACAGGCAGTGGGAACCCCCAGCCCCAGCACTCTCCAAATTCACCCTT150.13003901170351106No Hit
GGGCACCTTACTACCAGACAACCTTAGCCAAACCATCCG150.13003901170351106No Hit
GGGCACAACACTAAAGGACGAACCTGATCTCTTATACTAGTATCCTTAAT150.13003901170351106No Hit
GGGGAGTTTACTTACGTACATCCTACAGGAAATAACTTCAAAGTCACCTT150.13003901170351106No Hit
GCCTTCAACCTTTCAACCATGTATTTCTAGCTTTAGCTTAAATGCTGGAG150.13003901170351106No Hit
GGGAAGGTCAGCAGATAAACAAGTGAACAAAGGTCTCTGGTTTTCCTAGG140.12136974425661032No Hit
GGGTCGTCGCCGCCACGGGGGGCGTGCGATCGGCCCGAGGTTATCTAGAG140.12136974425661032No Hit
GGGGATCTCACACTGCCAAGGAAACTTAAAAATCACCCTGCTACTTTTCC140.12136974425661032No Hit
GGGGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG140.12136974425661032No Hit
GGGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCAC140.12136974425661032No Hit
GGGATACAGCATAACTACTAGAGTTCAGGCTCTTAGAGCTATTTCAACAC140.12136974425661032No Hit
GGGCAGAGTGTGCTTAACACAAAGCACCCAACTTACACTTAGGAGATTTC140.12136974425661032No Hit
GGTGTTTGTGTGAGAGGGTCTCGTTTTATTTTTTTCCTAAATAGATGGCC140.12136974425661032No Hit
GGGCTAATTTCCCCATAGCCGTGGGGTGACTTCCCTGGTCACCAAGGCAG140.12136974425661032No Hit
GGGAAGACTATTTAAAGTGGCACTCTCCACCACCCGTCATCTAAAAGAAG140.12136974425661032No Hit
GGGAATATCAGAAGGAGAATAAAGAGAGCAAGGAGCAGAAGCAATATTTG140.12136974425661032No Hit
GGGCAGTGCCAACCCATAGGGTGGATACAAAAGACAGGCAAGGAAGGGGT130.11270047680970957No Hit
GGGATTCTGGCTAATGGCATCACCTTCCACCTGTTCACATAATCCAGAAA130.11270047680970957No Hit
GGGACAAGAAGATACACACGTGCACAATGATCTGCTTAGCACTAATTGTG130.11270047680970957No Hit
ACTTCCTAACTCATTCTACCAACCCAGTTTTACCTTAATACCAAACCATCCG130.11270047680970957No Hit
GGGAGAAGAAATGGATCCTTGCAGAATAAGTGTGCTGTTTAAGTGGGAAG130.11270047680970957No Hit
GGGCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG130.11270047680970957No Hit
GGGGGCTTTCGCTCTAAGATTAAAAATGCCCTAGCCCACTTCTTACCACA130.11270047680970957No Hit
GGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAA130.11270047680970957No Hit
GGGCCAGGAAACTGCAGGGGTAATTTCTAGACATTTCTAATTGGCATTTT130.11270047680970957No Hit
GGGCAATCAATACTCATCATTAATAATCATAATGGCTATAGCAATAAAAC130.11270047680970957No Hit
GGGAGCACTCTACTTCTCATCTATATATAGAGTGCTCCTATCTCCTGCCA130.11270047680970957No Hit
GGGGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAA130.11270047680970957No Hit
GGGCTGATTCAGATGATGATAGCTGTCTTTGGAAACGCAAACGACAGAAA130.11270047680970957No Hit
GGGATTCCATCCCGTTCACTGGTTAAATCTTCACTCTCAAGCTCCATTTG130.11270047680970957No Hit
GGGGCAGAACCAACGAGGTGGCCGAATCTTCCTTCAGGATATCAAGAAAC130.11270047680970957No Hit
GGGATTATCCCGTTCTCTTCCTGGTGCCACTGTTCCAGTCAGATCTTCAT130.11270047680970957No Hit
GGGCTTTCTTCACCTTCCATAACTGCCCTTCCCTTTGCCAATCCTGCTCC130.11270047680970957No Hit
GGGAGAATATTTCCTAATTCATTCTGTGAGACCAATATTACCCTCATACCAAACCATCCG120.10403120936280884No Hit
GGGATCACCTAAAAAGCTGCTACCAAGACAGCCACGAAGATCCTACCAAA120.10403120936280884No Hit
AGGCATGAGCCACTGTGCCCAGACAGTAAAATACTTCTTATACTCAGTAA120.10403120936280884No Hit
GGGATGAACCATCACCTCTACTACCACCTCCACCACCATCACCATAACTA120.10403120936280884No Hit
GGGCGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCAC120.10403120936280884No Hit
GGGGGCTAATTTCCCCATAGCCGTGGGGTGACTTCCCTGGTCACCAAGGC120.10403120936280884No Hit
GGGAACACCAACAGCTCCTTTTCTACAAGAGGTGTATTGTTCCCATTCTC120.10403120936280884No Hit
GGGGAGGCCAGTGTTGTGAGCTTCCTGCTGGGACTGAGAAGGCTCACGAA120.10403120936280884No Hit
GGGAGAAGTGATGGAGGGTGCTGACAACCAGGGTGCAGGAGAACAAGGTA120.10403120936280884No Hit
GGGTCATGTCTCTCGATGTTTGTGTTTTCCATAGAGTCAGCTCATGTTCA120.10403120936280884No Hit
GGGAATTAACTCTTAGAAACTCCTAATCATACACGTTGACATAGCCACTG120.10403120936280884No Hit
GGGTAAAACCCGTCGTGGCTTGGATAGAATCCAGGGCTTGTGGCCTCTGG120.10403120936280884No Hit
GGGCAACCTACAGAATGGGAGAACATTTTTGCAATCTACTCATCTGACAA120.10403120936280884No Hit
GGGACCTAACCATATCCATGTGCCCCACCTCACTCTCAGCCTCAAACAGA120.10403120936280884No Hit
GGGCACGACACGTACTACGTTGTAGCCCACTTCCACTATGTCCTATCAAT120.10403120936280884No Hit
GGGTGGTGAGGTTGATCGGGGTTTATCGATTACAGAACAGGCTCCTCTAG120.10403120936280884No Hit
GGGCACAGCAAGACGAGAAGACCCTATGGAGCTTTAATTTATTAATGCAA120.10403120936280884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)