FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953535

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953535
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11535
Sequences flagged as poor quality0
Sequence length128-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1861.6124837451235372No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG1020.8842652795838752No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG760.658864325964456No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC710.6155179887299523No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC700.6068487212830517No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC680.5895101863892501No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC600.5201560468140443No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC580.5028175119202427No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC570.494148244473342No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC550.4768097095795405No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC510.4421326397919376No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG460.3987863025574339No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG430.3727785002167317No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG400.3467706978760295No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG390.3381014304291287No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC380.329432162982228No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT380.329432162982228No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC370.3207628955353273No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC360.31209362808842656No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC360.31209362808842656No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC350.30342436064152584No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG350.30342436064152584No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC350.30342436064152584No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC340.29475509319462506No Hit
CGGATGNTTNNGNTGGTGTCGTTNTGNCCNGAGAGTCCAGTTTGTATCTG340.29475509319462506No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC330.2860858257477243No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG330.2860858257477243No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC320.27741655830082357No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG320.27741655830082357No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC320.27741655830082357No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC320.27741655830082357No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG310.26874729085392285No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG300.26007802340702213No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC300.26007802340702213No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC300.26007802340702213No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC290.25140875596012136No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG290.25140875596012136No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC290.25140875596012136No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC290.25140875596012136No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC280.24273948851322064No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC270.23407022106631986No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA270.23407022106631986No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC260.22540095361941914No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC260.22540095361941914No Hit
CGGATGGTTNNGNTAAGGTTGTNTGGTAGNAAGGTGGAGTGGGTTTGGGG260.22540095361941914No Hit
CGGATGNTTNNGNTGGTGTCGTTNNGNCCTGAGAGTCCAGTTTGTATCTG250.21673168617251842No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC250.21673168617251842No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC250.21673168617251842No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAAGACAGG240.20806241872561768No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG240.20806241872561768No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCTGAGAGTCCAGTTTGTATCTG240.20806241872561768No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC240.20806241872561768No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC230.19939315127871696No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG220.1907238838318162No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC220.1907238838318162No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC220.1907238838318162No Hit
CGGATGGTTNNGNTGGTGTCGTNTTGTCCNGAGAGTCCAGTTTGTATCTG220.1907238838318162No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGTAAGGTGGAGTGGGTTTGGGG210.18205461638491546No Hit
CGGATGGTTNNGNTAAGGTTGTNCNCATTNACTCTGCGTTGATACCACTG210.18205461638491546Clontech SMART CDS Primer II A (95% over 21bp)
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC210.18205461638491546No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG210.18205461638491546No Hit
CGGATGNTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG210.18205461638491546No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG200.17338534893801474No Hit
CGGANGNTTNNGNTGGTGTCGTTNNGNCCNGAGANTCCAGTTTGTATCTG200.17338534893801474No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANCTCTTAGTAAGATTACACAT200.17338534893801474No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG200.17338534893801474No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC200.17338534893801474No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC190.164716081491114No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC190.164716081491114No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC190.164716081491114No Hit
CGGANGNTTNNGNTGGTGTCGTTNNGNCCNGAGAGTCCAGTTTGTATCTG190.164716081491114No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGTGGAGTGGGTTTGGGG190.164716081491114No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA190.164716081491114No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC190.164716081491114No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNAAGGTGGAGTGGGTTTGGGG190.164716081491114No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNGAGAGTCCAGTTTGTATCTG180.15604681404421328No Hit
CGGNTGGTTNNGNTGGTGTCGTNTNGTCCNNAGAGTCCAGTTTGTATCTG180.15604681404421328No Hit
CGGNTGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG180.15604681404421328No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC180.15604681404421328No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA170.14737754659731253No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGAGTTCCA170.14737754659731253No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG170.14737754659731253No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC170.14737754659731253No Hit
CGGATGGTTNNGNTGGTGTCGTTTNGTCCNGAGAGTCCAGTTTGTATCTG170.14737754659731253No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT170.14737754659731253No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC170.14737754659731253No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT160.13870827915041178No Hit
CGGATGGTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG160.13870827915041178No Hit
GTCTCTCAGNNGNTACTCTGCGNTNATACNACTGCTTCCGCGGACAGGCT160.13870827915041178No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG160.13870827915041178No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC160.13870827915041178No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG160.13870827915041178No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG150.13003901170351106No Hit
CGGATGNTTNNGCTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG150.13003901170351106No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG150.13003901170351106No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC150.13003901170351106No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT150.13003901170351106No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA140.12136974425661032No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC130.11270047680970957No Hit
CGGATGGTTNNGNTGGTGTCGTTNNGNCCNGAGANTCCAGTTTGTATCTG130.11270047680970957No Hit
CGGATGGTTNNGNCTAGGGTGTNGNCTGANAATAGGGGAAATCAGTGAAT130.11270047680970957No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTNAGTAAGATTACACAT130.11270047680970957No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAAGACAGG120.10403120936280884No Hit
CGGATGGTTNNGNCTAGGGTGTNGNCNGANNATAGGGGAAATCAGTGAAT120.10403120936280884No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNNAGAGTCCAGTTTGTATCTG120.10403120936280884No Hit
CGGANGNTTTNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG120.10403120936280884No Hit
CGGATTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC120.10403120936280884No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT120.10403120936280884No Hit
CGGATTGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG120.10403120936280884No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCTGAGAGTCCAGTTTGTATCTG120.10403120936280884No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC120.10403120936280884No Hit
CGGATGGTTNNGNTAAGGTTGTCTNGTAGNAAGGTGGAGTGGGTTTGGGG120.10403120936280884No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC120.10403120936280884No Hit
CGGATGNTTNNGNTAAGGTTGTNNNGNAGTAAGGNGGAGTGGGTTTGGGG120.10403120936280884No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)