FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953539

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953539
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23118
Sequences flagged as poor quality0
Sequence length288-300
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC5642.439657409810537No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2391.033826455575742No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC2250.9732675837010122No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC2120.9170343455316204No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC2020.8737780084782422No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC1970.8521498399515529No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1960.847824206246215No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC1910.826196037719526No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA1870.8088935028981746No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCGAGCGTGA1280.5536811142832425No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1250.540704213167229No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC1130.48879660870317504No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1100.4758197075871615No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC1090.4714940738818236No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1080.4671684401764859No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1080.4671684401764859No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC1070.46284280647114806No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA1070.46284280647114806No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1070.46284280647114806No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1070.46284280647114806No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1050.4541915390604724No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1050.4541915390604724No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC1030.44554027164979665No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1010.4368890042391211No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA960.41526083571243183No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACGTTTTTCA950.41093520200709405No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC880.38065576606972923No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC880.38065576606972923No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC870.3763301323643914No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACGTTTTTCA850.3676788649537157No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACGTTTTTCA850.3676788649537157No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC760.3287481616056752No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA750.32442252790033743No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA720.3114456267843239No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTAA710.3071199930789861No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACGTTTTTCA670.2898174582576347No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGGTTGA660.28549182455229694No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG650.2811661908469591No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA640.27684055714162126No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGTTTTA620.2681892897309456No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA600.25953802232026996No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATACGGATGAA580.2508867549095942No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA560.2422354874989186No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCTGA540.23358422008824295No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC520.22493295267756724No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCGAGCGTGA510.2206073189722294No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCGAGCGTGA510.2206073189722294No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCGAGCGTGA510.2206073189722294No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATCGAGCGTGA510.2206073189722294No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA500.21628168526689162No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCGAGCGTGA490.21195605156155375No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA470.2033047841508781No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGCTGGA460.19897915044554026No Hit
TGCTNCNGANNGNTCAAACACAGNGANCTCGGGTNGGAACACCTTGTTCA460.19897915044554026No Hit
TGCTNCNGANNGCTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA450.19465351674020245No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCGAGCGTGA440.19032788303486461No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACGTTTTTCA430.18600224932952678No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNGGGTGGGAACACCTTGTTCA420.18167661562418894No Hit
TGCTTCNGANNGCTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA420.18167661562418894No Hit
TGCTTCTGANNGNTCAAACACANCGACCTNGGGTGGGAACACGTTTTTCA410.1773509819188511No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA410.1773509819188511No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA410.1773509819188511No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTNGGGTNGGAACACGTTTTTCA400.17302534821351329No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATCAGGCCTGA390.16869971450817545No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA390.16869971450817545No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACGTTTTTCA370.16004844709749977No Hit
TGCTTCNGANNGNTCAAACACANNNANCTCGGGTNGGAACACGTTTTTCA370.16004844709749977No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNNGGTGGGAACACCTTGTTCA370.16004844709749977No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGCTTCA370.16004844709749977No Hit
TGCTNCNGANNGNTCAAACACAGNNANCTNGGGTGGGAACACGTTTTTCA370.16004844709749977No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA370.16004844709749977No Hit
TGCNTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACGTTTTTCA360.15572281339216196No Hit
TGCTTCTGANNGNTCAAACACANCNANCTNGGGTGGGAACACGTTTTTCA360.15572281339216196No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGTAAAA360.15572281339216196No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATACGGATGAA360.15572281339216196No Hit
TGCTTCTGANNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA360.15572281339216196No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC350.15139717968682412No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGGTTGA350.15139717968682412No Hit
TGCTTCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA350.15139717968682412No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTAA350.15139717968682412No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTAA350.15139717968682412No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGGTTGA340.14707154598148628No Hit
CGGATTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC340.14707154598148628No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA340.14707154598148628No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGACTTGA330.14274591227614847No Hit
TGCTTCNGANNGNTCAAACACAGNGANCTNGGGTGGGAACACCTTGTTCA330.14274591227614847No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGTTTAA330.14274591227614847No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGCTAAAA330.14274591227614847No Hit
GTCNCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCGAGCGTGA320.13842027857081063No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTAA310.1340946448654728No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACGTTTTTCA300.12976901116013498No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGGTTGA300.12976901116013498No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGGTTGA300.12976901116013498No Hit
CGGANTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC300.12976901116013498No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATCGAGCGTGA300.12976901116013498No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATTTGGTTTTA300.12976901116013498No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCTGGATATCGAGCGTGA290.1254433774547971No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATCAGGCCTGA290.1254433774547971No Hit
TGCTTCTGANNGNTCAAACACANNNANCTNNGGTGGGAACACCTTGTTCA290.1254433774547971No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG290.1254433774547971No Hit
GTCTCTCAGNNGNTACACGGCANGGTCAGNGTTCTGGATATCGAGCGTGA280.1211177437494593No Hit
TGCNTCTGANNGNTCAAACACANNNANCTCGGGTGGGAACACGTTTTTCA280.1211177437494593No Hit
TGCTTCTGANNGNTCAAACACAGCNACCTNGGGTGGGAACACCTTGTTCA280.1211177437494593No Hit
CGGNTGGTTNNGNTGGTGTCGTNNNGNCCNGAGAGTCCAGTTTGTATCTG280.1211177437494593No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNGTTCTGGATATCGAGCGTGA280.1211177437494593No Hit
CGGATTNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC280.1211177437494593No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTAA280.1211177437494593No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATAGGGCTGGA270.11679211004412147No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGTTTTA270.11679211004412147No Hit
TGCTTCNGANNGNTCAAACACAGNNANCTCGGGTGGGAACACGTTTTTCA270.11679211004412147No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG270.11679211004412147No Hit
GTCTNTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCGAGCGTGA270.11679211004412147No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGCTTCA270.11679211004412147No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATTTGGGTTGA270.11679211004412147No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTTA270.11679211004412147No Hit
GTCTNTNAGNNGNTACACGGCAGNNTNAGNGTTCNGGATATCGAGCGTGA270.11679211004412147No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGNCCNNAGAGTCCAGTTTGTATCTG260.11246647633878362No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTTGGAATGA260.11246647633878362No Hit
GTCTCTCAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATTTGGTTTAA260.11246647633878362No Hit
TGCNTCTGANNGNTCAAACACANCNACCTNNGGTGGGAACACCTTGTTCA260.11246647633878362No Hit
GTCTNTNAGCNGNTACACGGCAGNGTNAGGGTTCTGGATATCGAGCGTGA260.11246647633878362No Hit
GTCTCTCAGNNGNTACACGGCANGNTNAGNNTTCTGGATATCAGGCCTGA250.10814084263344581No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG250.10814084263344581No Hit
TGCTNCNGATNGNTCAAACACAGNGANCTCGGGTGGGAACACCTTGTTCA250.10814084263344581No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATACGGATGAA250.10814084263344581No Hit
GTCTCTCAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCAGGCCTGA250.10814084263344581No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCNGGATATTTGGCTTCA250.10814084263344581No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGTTTTA250.10814084263344581No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATAGGGAATAA240.10381520892810796No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATAGGGCTGGA240.10381520892810796No Hit
GTCNCTCAGNNGNTACACGGCANNNTNAGNGTTCTGGATATTTGGTTTAA240.10381520892810796No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNNTTCTGGATATCGAGCGTGA240.10381520892810796No Hit
GTCTCTNAGNNGNTACACGGCAGNGTNAGNGTTCTGGATATTTGGTTTTA240.10381520892810796No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATAGGGCTGGA240.10381520892810796No Hit
GTCTCTCAGNNGNTACACGGCANNNTNAGNNTTCTGGATATTTGGGTTGA240.10381520892810796No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATCAGGCCAGA240.10381520892810796No Hit
TGCTNCTGANNGNTCAAACACAGNNANCTCGGGTGGGAACACCTTGTTCA240.10381520892810796No Hit
GTCTNTNAGNNGNTACACGGCAGNGTNAGGGTTCTGGATATCAGGCCTGA240.10381520892810796No Hit
GTCTCTNAGNNGNTACACGGCAGNNTNAGGGTTCTGGATATCGAGCGTGA240.10381520892810796No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)