FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953542

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953542
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18617
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN681336.59558468066821No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC17889.604125261857442No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG4102.20228823118655No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG3902.094859536982328No Hit
GGGAGGACTCCTTTGTCCAAGATATATTCCGAAATCCTCCAACAGAGACC2821.5147445882795294No Hit
GGGGATGTTTACTGGTATCGGCAGCTCCCAGAGGAAGGTCTGAAATTCAT2071.1118869850136972No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1941.042058333780953No Hit
GGGATCAGACACAGAGTCTGAGTTCTGGGGCCTGGAACCTCAATGTGCAC1800.9668582478379975No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG1480.7949723371112425No Hit
GGGATTGTTTCTGACCAACAATGGCCTGCAGAGTGTAGTTTTTCTCTTTT1180.6338292958049094No Hit
GGGAAAAGCTGGAGGACTCAGCCATGTACTTCTGTGCCAGCAGATATACA970.5210291668904765No Hit
GGGGTTTGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAA970.5210291668904765No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT840.45120051565773217No Hit
GGGCAGCCACCATAATTTCTTACCTGGACTACCAAGAAGTCTTCTACCAG800.4297147768168878No Hit
GGGGATCCTGCCCTGACCCTGCCATGGGCACCAGCCTCCTCTGCTGGATG780.4189719073964656No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA780.4189719073964656No Hit
GGGTGCATTCCAGGGTCTGCAGTAAGACTCATCCTATGAGACAGGTACTT760.4082290379760434No Hit
GGGTTGATGTGTCCTCAGTGCTACTTATCTACTTCTTCCTACCCTCCCTG750.4028576032658323No Hit
GGGATTGCATTCCATACCATTCCATTCCACTCGGGTTGATTCCATTGAAT720.386743299135199No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT690.37062899500456575No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT680.36525756029435463No Hit
GGGATGGTGGAGACATTTGGGGCAGGAGAAGTGGCTGGCTCATTCTTTAC660.3545146908739324No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT580.31154321319224365No Hit
GGGACTTAAACTACACCCTACAACAAATGGACTTAACAGCTATTTACAGA570.30617177848203253No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC570.30617177848203253No Hit
TTTGTAAGAGTTCTTGAAAATTTTTACATTCTATGATGTATTCATTTAAT550.29542890906161035No Hit
GGGATATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAA550.29542890906161035No Hit
GGGTGTAATGAATACCAGAGAGAGATGAGACAGAAAATCTTGAAATTAAA530.28468603964118816No Hit
GGGCTTGATTCATTTCCATTCTAATCCCTTCCATTCCATTCCATTCCACT530.28468603964118816No Hit
GGGGGGACTTGCCTCTGTAATATAAAACAAACAATAAAACTTGATACGTG510.273943170220766No Hit
GGGAGAAAATCTGCCGCATCTGTTCCTGGCTAAAGACCCTAACCGGCTTC490.2632003008003438No Hit
GGGGTGGGCTCTGAGGCTGCAGCAGGGTTTTTCTCAGAATTCCTGTCCAG490.2632003008003438No Hit
TGGGATACGTACTATTGCATTTCTCTCTATATTGAAGCCCTTGCTTCATA480.25782886609013267No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG470.25245743137992155No Hit
GGGAGGCTTAGTATCTCTGATACTCTTACTGCTTGGAAGAACCCGTGGAG460.24708599666971048No Hit
GGCATCACTATCCACACATTTTCTCAAGCCAGAAATCTTAGAGTGTGGCA460.24708599666971048No Hit
GGGAAGTCTAACAAATCAAAGATAGGGCATGACTATAAAGTAATTAACCAATCATACCAAACCATCCG460.24708599666971048No Hit
GGGTAAGTTTCTGCTCTCATATGCACATATGTCTATACTTCCCATTTATT460.24708599666971048No Hit
GGGGAGGGTTTCCTACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGG450.24171456195949936No Hit
GGGGCTATCCTCTTCAACAATATACTCTCCGGGCAATGAACCATAACCAA450.24171456195949936No Hit
GGGAATGAGGAGGATATAAAAAATGGAAAGATATTTCATGCTTATGAAAT440.2363431272492883No Hit
GGGGGTGTGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGG440.2363431272492883No Hit
GGGAGCGCCCGAATCTGGCTTGGCGAAATATCTCTTTGACAAGCACACCC440.2363431272492883No Hit
GGGATGAATCCCACCCCATTCCTCACAACAGCAGCTGGTTTGAAATAACC440.2363431272492883No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTT440.2363431272492883No Hit
GGGAAAGGAGAATTTAAAACAAATGGTAGGAATCCACAAGATTTTCATGT430.23097169253907718No Hit
GGGAATTGACAATATAGATATGGAAGCCACCAAAGGAAAAACTGAAGAAT430.23097169253907718No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG430.23097169253907718No Hit
GGGACTTTCGGGAAGGGGACCAAACTCTCGGTCATACCAAAGGCCTGAGT430.23097169253907718No Hit
GGGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTCCAAGGCAG410.22022882311865502No Hit
GGGCACCATTCAGGCAGACCCCACTCTCCACTTTGTCCAAGCATGTTCCT410.22022882311865502No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG410.22022882311865502No Hit
GGGGTTTTAGACATCGCAATACCTCTGGCATTCTTTCTATTCCTCAAACA400.2148573884084439No Hit
GGGAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGAAGGAGAAGGAAATG400.2148573884084439No Hit
GGGAAAACAGCCCTAAAAGCTGCTCCCATACTAGCTCTCCCTAACTTGGA400.2148573884084439No Hit
CATCTGGAAGCGGTTCCCCAGACAGGTTAACATTTCCTGAGCTCAGTCCCTCCTCATACCAAACCATCCG390.2094859536982328No Hit
GGGTGAGACACATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGG390.2094859536982328No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA380.2041145189880217No Hit
GGGGACACAGAGCCAAACCATATCACATAGTTTCCAATTTTCTTGTATTG380.2041145189880217No Hit
GGGGGAAACCATCTGCCCTTGTGAGCGACTCCGCTTTGTACTTCTGTGCT380.2041145189880217No Hit
GGGAAAAACATTGAAGACGTCATTGCCCAGGGTATTGGCAAGCTTGCCAGTGTACCAGCTGAGAGAC380.2041145189880217No Hit
GGGGCTAAACCTAGCCCCAAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG370.19874308427781062No Hit
GGGAACCCAGAACGCTGTTTTATTCCTGTCCCCTTTCTCCGTCTTTCTAC370.19874308427781062No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA370.19874308427781062No Hit
GGGGGCCTGTTGTTGAATTTATATTTCCAAACACTCAATTCTAACTTTAT360.1933716495675995No Hit
GGGTGTTTCTAGCTCTAAAATCCACATTTACCTGCATTTTTTCCCCCTCT360.1933716495675995No Hit
GGGATAAATGCTCCAATTAAAAGACACAGACTGGCAAATTGGATAGAGTC350.1880002148573884No Hit
GGGGTGATCAGATTTTTGTCTTTCATAGTCAATAGTCTAATGTTATGGAA340.18262878014717732No Hit
GGGAAGGGAAGAGTGGGAAGGACTGCATATCTTGGTTTGTGTACCAGCTGAGAGAC340.18262878014717732No Hit
GGGTCACTTGGGGGTGGCATCTTCATCAGTAAATTACATTTACTTTCTCC340.18262878014717732No Hit
GGGAGTGATTCAGCCACCTACCTCTGTGTGGTGGTCACGGGAGGAGGAAA330.1772573454369662No Hit
GGGAATCAGATCTGTCTTGATGTGTCATCTAATTAAGGGAATCCCTTGTA330.1772573454369662No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT330.1772573454369662No Hit
GAGAATGGACACAAGCAGGTCTTGGTACAAGGAGTAGACTCTAGCAAACA330.1772573454369662No Hit
GGGGAGCCGGGTTGTATTTGGAAACGCGGAGTGAGTTTTTCCGTGCTGTG330.1772573454369662No Hit
GGGTTGATTCCATTCCATTCCACTCCATTCCGTTCCATTCCATTCCAATG320.1718859107267551No Hit
GGGACAGATTATTCCTTAATTCTTATTAAGCAAAGAAGCCTTCATCCATT320.1718859107267551No Hit
GGGATAGGAGGCTTCATTCACTGATTTCCCCTATTCTCAGGCTACACCCTAGACCAAACCATCCG320.1718859107267551No Hit
GGGAGGGGATGTAACTTACATACCGTAAATTGTACAGATACTAAGTATAT320.1718859107267551No Hit
GGGGGATGGATAGCAGCTATAAATTGATCTTCGGGAGTGGGACCAGACTG320.1718859107267551No Hit
GGGCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG310.16651447601654404No Hit
GGGGACAGTGTAAATACCGGCACTGCCAGTAAACTCACCTTTGGGACTGG310.16651447601654404No Hit
GGGAGAGGTAACAATGACTACGAATTCTGCAGATATTAAAAGGTCAGTAA300.16114304130633292No Hit
GGGAATATCACACTCTGGGGACTGTTGTGGGGTGGGGGGAGGGGGGAGGG300.16114304130633292No Hit
GGGTTAAAGCACTCCAGCAACTTTTGATTACACTCAGAAACTAACACCAA300.16114304130633292No Hit
GGGAGGGCGACCTCGCACTGTGGTTCTAACGACTACAAGCTCAGCTTTGG300.16114304130633292No Hit
GGGGGGAAGACTGACTGCTCAGTTTGGTATAACCAGAAAGGACAGCTTCC300.16114304130633292No Hit
ACCCCATTCTCCATGATGTTCTTATTTCATATTGCATGCTTGTATTATACCAAACCATCCG290.15577160659612183No Hit
GGAATCCATTCCATTCCATTCCATACCAAACCATCCG290.15577160659612183No Hit
GGGGACAGTGACACTGATCTGGTAAAGCCCCCATCCTGGCCTGACCCTGC290.15577160659612183No Hit
GGGAATACACCTACCCTTCTTCCAGGATTCCCTTTCTCTCCAGAAGAACC280.15040017188591073No Hit
GGGTGAGCACATCAAGAAGAGGGGTGAAGTGACAAAAGGGAGTTTATTGT280.15040017188591073No Hit
GGGTCAGTCAAGTCAGAGAGAGCAAACAAGTGGAAGACTTAATGCCTCGC280.15040017188591073No Hit
GGGGAATCCTCAGTGAACCAGGGCAGAAAAGAATGATGATATCCTTGAGA280.15040017188591073No Hit
GGGAGAGGAGTTTATGTAAAGGGTTGGCCCAGAGTGTGTATTCAGGAGGA270.1450287371756996No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA270.1450287371756996No Hit
GGGGTCTCAGCAGCCACATTTATGGTCCTGTTGCCCTGTTGACCTCATGA270.1450287371756996No Hit
GGGCGTGGTGGCTCACACCTGTGATCCCAGCACTTTGGGAAGCCAAGGTG260.13965730246548852No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT260.13965730246548852No Hit
GGGCCCTCATGCCTGAGTTCATTCAAGTTTTAGCTGTGTCTTCAGCTCCC260.13965730246548852No Hit
GGGAAACCCCGTCTCTACTAAAAATACAAAAATTAGCCAGGTGTGGTGGT260.13965730246548852No Hit
GGGAGACGTTCTTCGCCGAGAGTCGTCGGGGTTTCCTGCTTCAACAGTGC260.13965730246548852No Hit
TCCAATCAGGTTGACTCCATTCCATTCCATACCAAACCATCCG260.13965730246548852No Hit
GGGGTGACTTCCTTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT250.13428586775527743No Hit
GGGGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAGT250.13428586775527743No Hit
GGGACTACGCTCACTGTGAAGCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC250.13428586775527743No Hit
GGGGAGCTGGAAACACCTCCATCCTGCCTCTTCATGCCATGGCCTCCCTG240.12891443304506633No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG240.12891443304506633No Hit
GGGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCATGCCCTG240.12891443304506633No Hit
GGGTTGACTTTAGAAAGCCTTGGTATCTGACCGGTCATGCTGCCAGTCTG240.12891443304506633No Hit
GAGTCCATTCCATTCGTGTCCATTCCATTTGGGTCCATTCCATTCCATTG240.12891443304506633No Hit
GGGGACTTTCGGGAAGGGGACCAAACTCTCGGTCATACCAAATATCCAGA240.12891443304506633No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC240.12891443304506633No Hit
GGGGGTATTACTGTGACAATAACAATGACATGCGCTTTGGAGCAGGGACC230.12354299833485524No Hit
GGGGACAGTGATAATGAAAGCTAAGCCTCGGGCTAATTTCCCCATAGCCG230.12354299833485524No Hit
GGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTGTTC230.12354299833485524No Hit
GGGGGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGAT230.12354299833485524No Hit
GGGGTAGAGCAGACTGTGGCTTCACCTCCGAGTCTTACCAGCAAGGGGTC230.12354299833485524No Hit
GGGGGAAAGAGAGAGAATGGGAAGGGCGATAAGAGGAGGGGTTAGTGATA230.12354299833485524No Hit
GGGCAGGCATGCTCATAAGGAAAGGTTAAAAAAAGTAAAAGGAACTCGGC230.12354299833485524No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA220.11817156362464415No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT220.11817156362464415No Hit
GGGGGGATTGAAAATTTTAATCCTCAGTGAACCAGGGCAGAAAAGAATGA220.11817156362464415No Hit
ATTCCACATACAAATTTTTTGAGGTTATCCTCATTGATCCATTCCATAAA220.11817156362464415No Hit
GGGGCAAGCAGCCTTGTCTTAATCATATCACATTCTCTTACAATTTTGTG210.11280012891443304No Hit
GGGAGCATGAGTACTCCTTTCAAGACCTCACCTACCTCTACCTATACTCA210.11280012891443304No Hit
GGGTTATCACCACCACGCCACAAAAATAGAAACAACCATCAGAGAGTACT210.11280012891443304No Hit
GGGCCAGGGACACGGCTCACCGTGCTAGAGGACCTGAAAAACGTGTTCCC210.11280012891443304No Hit
GGGATAGCAACTTCCAAGCTCACCTTCAAGTCATCGTTATATTTTTACAA210.11280012891443304No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG210.11280012891443304No Hit
GGCGTGAACCCGCCAGGCGGAGCTTGCAGTGAGCGGAGATCGCGCCACTG200.10742869420422195No Hit
GGCTCTCCTAAAAACAACCCACCCTTGTCCAGCTGCCTTCCCTCCTGAGG200.10742869420422195No Hit
GGGTAATGGGAATTCCACAAAGGGAAGAAAGAGGAAAACAAACAGAAGCA200.10742869420422195No Hit
GGGGGCTGCTGGGCTCATTGCAGCTCAGACACAGCAAAAGAGCCTAGAAC200.10742869420422195No Hit
GGGAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTTCC200.10742869420422195No Hit
GGGAGAGCACTTACTTTTGGGAGTGGAACAAGACTCCAAGTGCAACCAAA200.10742869420422195No Hit
GGGGTGAGTAGGCTGACGACAGAAGCAGATGCCTCTGTGGACAGTTTAAG190.10205725949401084No Hit
GGGATTCCATTCGATGACTCCATTTGATCCCATTCGATGATTTCCTTTGA190.10205725949401084No Hit
GGGCAAGGACTTCATGTCTAAAACACCAAAAGCGATGGCAACAAAAGCCA190.10205725949401084No Hit
GGGGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTATCT190.10205725949401084No Hit
GGGGAGTCCATATAAGCGACACGGCTGAGTACTCGCCATATGGTGGAGGT190.10205725949401084No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA190.10205725949401084No Hit
GGGAGAGACAGGACAGGAAAATCGTAGGTATTCCAGTAAAACTACTTCTA190.10205725949401084No Hit
GGGGAAGCCTCACACAGCCCAGTAACTTTGCTAGTACCTCTTGAGTGCAA190.10205725949401084No Hit
AGGCAACCTACAAAATGGGAGAAAATTTTCGCAACCTACTCATCTGACAA190.10205725949401084No Hit
GGGACAGATACGCAGTATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGA190.10205725949401084No Hit
GGGGAAACCCTCAGCCCATATGAGCGACGCGGCTGAGTACTTCTGTGCTG190.10205725949401084No Hit
GGGCCCCTCGTGGCCAGGGGGACCCCAAAAAACCTTAAAAACTGAATTCC190.10205725949401084No Hit
CAGGCACCACACTAAACTCTTCATAGGAAGTATCTAATTTATGCCTCATACCAAACCATCCG190.10205725949401084No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)