FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953544

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953544
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9060
Sequences flagged as poor quality0
Sequence length35-300
%GC47

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN387942.81456953642384No Hit
GGGAAACAGAATGGCTTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGGC196521.688741721854303No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC1561.7218543046357615No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1461.6114790286975718No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG1411.5562913907284768No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG1171.2913907284768211No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT650.717439293598234No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT650.717439293598234No Hit
GGGGAAAAATTCTAGGCTATATACAACTACGCAAAGGCCCCAACGTTGTA540.5960264900662252No Hit
GGGACAGATACAGAAGACCCCTCCGTCATGCAGCATCTGCCATGAGCATC540.5960264900662252No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC510.5629139072847682No Hit
GGGAAACAGAATGGCTTTTGGCTGAGAAGGCTGGGTCTACATTTCAGGCC360.3973509933774834No Hit
GGGAATGCATTGTTTTATTTATTGTATCTATATCTTGGGATAACTGCTAT340.37527593818984545No Hit
GGGAAGCAGCTGGGCCTTATCAGTTCTTTCCTTGGTTATATCCAGCATCC300.33112582781456956No Hit
GGTATCTTAATTTTTCCAATATTTGTAATTGTTTTTTGATATGGATAAAT300.33112582781456956No Hit
GCACTCCACTCCATTCCATTCACTTCAATTCCATTGCACTCAACTCTATT290.3200883002207506No Hit
GGGGATTTTGGCCCAGGCACCCGGCTGACAGTGCTCGAGGACCTGAAAAA280.3090507726269316No Hit
GGGACGAACCTGATCTCTTATACTAGTATCCTTAATCATTTTTATTGCCA270.2980132450331126No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT270.2980132450331126No Hit
GGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCATCCG230.25386313465783666No Hit
GGGGAGCAGTTTTGTTGATTTAACTGTTGAGGTTTAGGGCTAAGCATAGT230.25386313465783666No Hit
GGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG230.25386313465783666No Hit
GGGGAGTGACAGGGTTTGGCTGTGTCCCCACCCAAATCTCATCTTGAATG230.25386313465783666No Hit
GGGCCACTCCACTCCATACTATTATATTCCACTCCACTCCACTGCAGTCC220.24282560706401765No Hit
GGGATTACAAGGTCAGGAGTTCGAGACCAGCCTGGCCAACATGGTGAAAT210.2317880794701987No Hit
GGGGAGATGGGACCAGGAGGGACAGAAAGACAGAGGGCTGATGGTTGGGA210.2317880794701987No Hit
GGGAGGCTTTGAAGGTGAGTGAGGGATGATAACAGGTCTATGAAGAAGTT210.2317880794701987No Hit
GGGAAACCCAGCCTAAGGGCCCACCAATACTGAACCGGTCACCACCATGC210.2317880794701987No Hit
GGGGACACAAAGAAGGGAACAACAGACACAAAGGCCTACTTGAGGGTAGG200.22075055187637968No Hit
GGGACATATTTCCTCTTCTTCTACCTTTAGCTTTTATTCCACAGCTCTTG200.22075055187637968No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA200.22075055187637968No Hit
GGGAGCAAATGACCAGGACTGGTCAAGATTATATATATATATGTATATAT200.22075055187637968No Hit
GGGGATTTTTTTTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTT190.20971302428256072No Hit
GGGGTTGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA190.20971302428256072No Hit
GGGATCTAATGAAACCAAAGAGCTTCTGCATAGCAAAAGAAACTACCATC180.1986754966887417No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG180.1986754966887417No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA180.1986754966887417No Hit
GGGCCAAGACACCCACCATCCAACGGTTCTGTTGGTTCCCAGCATATCTG170.18763796909492272No Hit
GGGGTTGCTGGCGCACACCTGGGTTTGGTGGCACACACCTATAGTCCTAG170.18763796909492272No Hit
GGGTTTTTTCTGGGTCTCCAGTGGAAACCAATACTGCCAACACATTCATT160.17660044150110377No Hit
AGGTGTGCCAGAAAAGCATGAAACCTGCTGTTAAGTTCTCTGAAACTCAC160.17660044150110377No Hit
GGGGGTCCTTCTTCTGGTACAGACAATATTCTGGGAAAAGCCCTGAGTTG160.17660044150110377No Hit
GGGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG160.17660044150110377No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG160.17660044150110377No Hit
GGGCATCAAAGAATTGGGTGACCACGTGACCAACTTGCGCAAGATGGGAG150.16556291390728478No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGAAAAAAAAT150.16556291390728478No Hit
GGGAGTAAAACTTGTCATCCCTACTATCTTCTGTCTAGTCATACTCCTAT150.16556291390728478No Hit
GGGGAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTAAAGTC150.16556291390728478No Hit
GTGGTACAATTTGCTTGATTTCTCTCATATTATTGAACACTTGGCATGCC150.16556291390728478No Hit
CCATTGCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATT140.1545253863134658No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCGGAAGC140.1545253863134658Illumina RNA PCR Primer (96% over 27bp)
GGGAATATTCAACTCTGTGACTTGAATGCACACATCACAAAGAAGTTTCT140.1545253863134658No Hit
GGGTGAGACAGAGTCCTCCCTGGGACATCTGGAAAAGCCCAAGGAAGAGA140.1545253863134658No Hit
GGGTCCATGGATGTTTCTCTCCCTGTTTTCCACCACCTCTTTCTCTATTC140.1545253863134658No Hit
GGGCATCGCTGCTGACTGAGGAATGGAACACCGGGACAAGACCTTCTGAA140.1545253863134658No Hit
GGGACAAAGAAGGGCATTACATATTAACAAAAGGTTATATTCAATGAGAA130.1434878587196468No Hit
CAGCCTTGACTCTTTCCACCCTCTCATATCTCATACCAAACCATCCG130.1434878587196468No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGCTAATAGAAAGGCTAGGACCAAACCATCCG130.1434878587196468No Hit
GGGCCACGCTGGCCCCATAGCTCAGACTTTTAACATTGAAAAAGTCTTGA130.1434878587196468No Hit
GGGAGAGGTGGTGGAGGTAGCAATTAACAGCAGTGGTAGTAGTATAGTAG130.1434878587196468No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT130.1434878587196468No Hit
GGGAATGTTCAACTCTGTGACTTGAATGCACACATCACAAAGAAGTTTCT120.13245033112582782No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT120.13245033112582782No Hit
GGGGTCTCCTCTATCTTAGGGGCCATCAATTTCATCACAACAATTATCAA120.13245033112582782No Hit
GGGAGGAAGTTTGATTTTGACTTCCCTGGTCACCAAGGCAGTGCATGCAT120.13245033112582782No Hit
GGGATTTACAGGTGTGAGCCACTGCGCCCAGCTTCTTCCTCCATTTTCTT120.13245033112582782No Hit
GGGAAGCTGGCTTGACAGAGATAAGTTATATTGGAATCTAACTGTCAGAG120.13245033112582782No Hit
GGGATGGAATGGGGTGGAGTGGAGTGGAATGGAGTGCAGTCCAATTCATT110.12141280353200883No Hit
GGGACTGCTGTATATCTTGGTTGTGGCCACCACCACAACCAAGATATACA110.12141280353200883No Hit
GAGGGCTCACACTCTCGTCTTCTGGTCACCTCTCACTGTGGCCCTTCAGC110.12141280353200883No Hit
GGGAAGGAGGTGCACAGTGGGGTCAGCACTGTGTTCCTAAAAGTGATTCT110.12141280353200883No Hit
GGGAAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATCACCTAAAAAGCT110.12141280353200883No Hit
GGGAATAGATTCCATTCGATGATTCCACTTGATTCCATTTACGATGATTC110.12141280353200883No Hit
GGGGGAAGGCAAAAGGCACATTTTACATGGCAGCAGGCAAGAGAGAATGA110.12141280353200883No Hit
GGGAAATTTATAGCACTAAATGCCCACAAGAGAAAGTAGGAAAGATCCAA110.12141280353200883No Hit
GGGAGTATATCTTGCAGAATCATTTATGTAGAGACTTAAAGTTTACCCAG110.12141280353200883No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCAGAAGCA100.11037527593818984Illumina RNA PCR Primer (96% over 27bp)
GGGGAACTGAGGCTCAACTGAAAGCAGCAGGAAATTCAAAGGTATTTATA100.11037527593818984No Hit
GGGGGAAGGGGATGGTGGGAACAAAATGCCAGAACCAAACCATCCG100.11037527593818984No Hit
GGGATTACAGGCATGAGCCACGGTGCCCAGCCAGAAATAATTCCTACTTC100.11037527593818984No Hit
GGGGACTTGGGAATGGATTTATCATATGTGATTCACCCACCTCAAAATTA100.11037527593818984No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA100.11037527593818984No Hit
GGGGGAGAGAGAGAGAGAGAGAGAGAGAGAATGCTCCCAGGATGGAAGCT100.11037527593818984No Hit
GGGGCCATAAACAGGGCTGTGGCTGGATGACACAGCAGGCAGCATCCTGA100.11037527593818984No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG100.11037527593818984No Hit
GGGAGAGATACCAGGGCAAAGGAAACGGAGTTCAATAGGAAAGAGGACAA100.11037527593818984No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)