FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953546

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953546
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN635445.47341301080656No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG5814.158019036713663No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC4923.5210763615544263No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG3992.8555070493093826No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT3302.3616975595791883No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG3132.240034351964503No Hit
GGGAATAACGCCGCCGCATCGCCGGTCGGCATCGTTTATGGTCGGAACTA2261.6174049953481713No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT1270.9088957274744149No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG1110.7943891791311817No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA1070.7657625420453732No Hit
GGGGTATCTAATCCCAGTTTGGGCTTTAACATATCTGAACACACAATAGC1000.7156659271452086No Hit
GGGAACTTCAGCAGAGAGATGGAAATATAAAGAGACAAATGGAAATGCCA880.6297860158877836No Hit
GGGAGGACAGCAAATGGAGCCATTCTGATTCTGACAAAAAGTCCAGAACC870.6226293566163315No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG790.5653760824447148No Hit
GGGGCTAAGCATAGTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTG770.5510627639018106No Hit
GGGGTAGATAGTAGAAAAGACGATAAAGACTGAAAAGAAATGGCGAGGAC730.5224361268160023No Hit
GGGAGGAAGGAAGGAAGGAAGCAAGGAAGAAAGGAAGGAAGGAAAGAAGG680.48665283045874186No Hit
GGGTAGCAATAAAACTAGGAATAGCCCCCTTTCACTTCTGAGTCCCAGAG600.4293995562871251No Hit
TTCATTCCATTCCATTCCATTCCCTTTCATTCCATTCCATTCCTTTCCAT600.4293995562871251No Hit
GGGGAGTTGATTCCATTGCTTTGCATTCCATTCCATTCCATTCCATTCCG580.41508623774422104No Hit
GGGAGATTTCGCGTGCTCTGAGCACCATCCGCATGCAGGGGCCTGGTAGA580.41508623774422104No Hit
GGGTGCCAAAGGGCCGGGGGCACCATAGCTGGAAGGGCAGGAGGCTCCCA570.4079295784727689No Hit
GGGAGACCGGAAGCGCTCCGCTGTACCTGGATCCTGCTCCTCTGGGTTGA530.3793029413869606No Hit
GGGGAGGATATGTAACACAGTGTGATTTATAACCAGGGAGGAAAGCTTAT490.3506763043011522No Hit
GGGTGGATGGGTGGGTGGATGGGTAGACCCCACTCACTCATCCATCCACC470.3363629857582481No Hit
TCCACTCTATTCCATTCGAGTCCATTCCATTGCATTCCATTCCATTCCAT460.329206326486796No Hit
GAAACTTGTGGAGAGCTTATACAGAGCCTCTGGGGGAAAAAAGAGCCCTT450.32204966721534384No Hit
GGGCAAATACAAGATCAACACACAAATTTGTGGAAATATACAACCCTCCT440.3148930079438918No Hit
GGGAAGGTATCTTGTGTTCATGGATTGGTGGACAATATTATGAAGATGGA410.2934230301295355No Hit
GGGAGAGTGAGAATAAAATGGTCTGTGAGCAGAAGCTCCTGAAGGGAGAG400.28626637085808343No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTAAAAAAAAAT390.2791097115866314No Hit
GGGATAAAAGACTACACATTGGGTACAGTTCTTTAGTGGTGACAGGTGCA390.2791097115866314No Hit
GGGGAGACACAGTGCTGCCTGCCCCTTTGTGCCATGGGCTCCAGGCTGCT380.27195305231517924No Hit
AGTACCCAGACTTCAAATCACAGTCCCCAGAGCAGGACCCCCGCACCTGC370.2647963930437272No Hit
GGGGATGACGATGTCGATACCAAGAAGCAGAAGACCGACGAGGATGACTA360.2576397337722751No Hit
GGGCGCATAAGCCTGCGTCAGATTAAAACACTGAACTGACAATTAACAGC360.2576397337722751No Hit
GGGGACCTAAAAAGCTGCTACCAAGACAGCCACGAAGATCCTACCAAAAT340.24332641522937093No Hit
GGGAGGTCTCAGGAAACTTAGGACTATCATGGCAAAAGGCAAGCAAGGAC340.24332641522937093No Hit
GGGGTTTATCGATTACAGAACAGGCTCCTCTAGAGGGATATGAAGCACCG330.23616975595791884No Hit
GGGGCTGTGAGCCAGTGGCCCTACTCTCATCTCAGCTCTGCCACTGATGG320.22901309668646677No Hit
GGGAATGTGGAAACAATGGAGGAAGTCCCTGCCTTTGGGGGCAGAGACCA320.22901309668646677No Hit
GGGTCGCATCTCTGTGTGCCACACAGAACTTTTAGACAAGAGTAGAGGGG320.22901309668646677No Hit
GGGCTTGCTGGGAGGAGGTGTTGGCTATTGGTGTTGGTTGTTTGGTGTTG300.21469977814356256No Hit
GGGAGGAACATGATCCGACTTACGCTGTAGACCAGCTCTGGCTGCGGCAG300.21469977814356256No Hit
GGGGGCAGTATCATATATGGACAAGCATGCTCTGGAGACTACCATCCATT300.21469977814356256No Hit
GGGAGTAAAAAGAAATTAATACACATATGCATACCAAACCATCCG280.2003864596006584No Hit
GGGATGAAATTAAGGGAGTTCTCCAAAAATAATTGTTCCCACCTAAAAAA270.19322980032920634No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT260.18607314105775424No Hit
GGGCGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT260.18607314105775424No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT260.18607314105775424No Hit
GGGGTCTGGGACAGTGGGGGTGGCTGCCTAGGTGCAGGCCCCCATGTGAA250.17891648178630215No Hit
GGGTTTTTTTAATGACATTTTGCCTCAGCAGTTCTGAATTCCTAGTTACG250.17891648178630215No Hit
GGGGAGGGGGTGATCTAAAACACTCTTTACGCCGGCTTCTATTGACTTGG250.17891648178630215No Hit
GGGGTGGAGTGAAGTGGAGTGGAGTTGAGTGGAATAGAGTCTAAATTCCA250.17891648178630215No Hit
GGGAAATATGAAGAAAACAAACTGAAATGGTATCCCATGCCAAGAACAGA250.17891648178630215No Hit
GGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTCAGATATGT250.17891648178630215No Hit
GGGAAAGCTCCTTCATTCCCATAGCTTTAACTATCACTTCCATGTAGATGACTCATACCAAACCATCCG240.17175982251485006No Hit
GAGCTAGTTGTTGATATGCTTTAGTGGGCTTGGCAAGAGTGAGTAAAGAG230.164603163243398No Hit
GGGGAGACACATTACCTGAATGAGCAGGTGAAAGCCATCAAAGAATTGGG230.164603163243398No Hit
GGGGGGAAAAGCCCCCTATAAAACCATCATATCTCATAAGAATTCACTAT230.164603163243398No Hit
GGGGAGGCCAGTGTTGTGGGCTTCCTGCTGGGACTGAGAAGGCTCACGAA220.1574465039719459No Hit
GGGATTATATCAAACTAAAAAGTTTCCGCACAGGAAAGGAAATAATCAAC220.1574465039719459No Hit
GGGGTGGACAGGAGGCAGCAGTTATTCCTGTCCTGGGAGGCAGGCAAGAA220.1574465039719459No Hit
GGGGCCAGGGCAGGGATGGCAATGAGCAGAAGAGCTTGTGGCTGAGTCAG210.1502898447004938No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA210.1502898447004938No Hit
GGGTATCTTATACACACGCAGGCTTATTGTTCAGAATAGATAATTTACGA210.1502898447004938No Hit
GGGGAGGGATGATGGGGGAGAGCTGGTGGTTGCTGAGTGTCCAGGTGTTT210.1502898447004938No Hit
GGGGCTGAGGTCAGACGGGTTCGGCCCTTGGCCATGGCAGCTTGGTTGGG210.1502898447004938No Hit
GGGCTCCTCACTCCTTCTGCCTCTCCTACTCTCATCCCTTCTCTTCCCCT210.1502898447004938No Hit
GGGTAGGTCCTTTAGACATCATCTTTAGCTCTTATAACTGGGCACACTTG210.1502898447004938No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG210.1502898447004938No Hit
GGGGTGAACCCAGGAGGCAGAGCTTGCAGTGAGCCAAGATGGTACCACTG200.14313318542904171No Hit
GGGGAGCAGAGGCGATGCAGGCCGGAAAGTCACCATGGTGCGTGTTTCCG200.14313318542904171No Hit
GTAGCGCCAAACTTCTTCAAATAGACCCATCTCCAAACCATCCG200.14313318542904171No Hit
GGGGCAGGGAGAAAGCCTGGAGCTTTGTGTTTGTTTGGACGATCAATATC200.14313318542904171No Hit
GGGCAGAAGGAGGCATTTGAAGGTTAGATCTCTTCAGAAAACCTCCTCTT200.14313318542904171No Hit
GGGATTTTTTTAATTTTTTTTTTTTCTGACAACTTGCAACCATACAACTT200.14313318542904171No Hit
GGGAGAGTCTCTGGTACTAGCTGCTGTAGCAGTGCCCTTCATCCAGGGCA190.13597652615758962No Hit
GGGGATAGACACCTGACTGTAGTGCCAGGGCCAGGAATAAATCCTCACTC190.13597652615758962No Hit
GGAATAGAATAGAGAACCTAGAAATAAATCCATGTATTTACTAATATTTG190.13597652615758962No Hit
GGGAAAGGAGAGAGGGATGAATGGGTGGACCACAGGTGATTTTTAGGACA190.13597652615758962No Hit
GGGGGGGCCATCAATTTCATCACAACAATTATCAATATAAAACCCCCTGCCATAACCCAATACCAAACCATCCG190.13597652615758962No Hit
GGGTGGTATGATCCTTATTGTGCCAAAGATAGGAAAGAAGTTTGGAGAGA180.12881986688613756No Hit
GGGCCTTCTGCAAAGGGTGCTTGCCCATGAAGTTGCCCTGCCCCTTCTTC180.12881986688613756No Hit
GGGCGCGGCCGGGCGGGCCGCGGGGCGGGCTCCCGGCCCCGGCCGACGCG180.12881986688613756No Hit
GGGATGACCCCAATACGCAAAATTAACCCCCTAATAAAACTAATTAACCA180.12881986688613756No Hit
GGGCATGGTGGATCACACCTGTAAGAAAAGATCATTTTTAAAAACTTTTG170.12166320761468546No Hit
GATACGGCGACCACCGAGATCTACACGAGCCTTAGCCTGTCCGCGGAAGC170.12166320761468546Illumina RNA PCR Primer (96% over 27bp)
GGGAGGTGAAAGGCACGTCTTACATGGTGGCAGACAAGAGAGAATGAGAA170.12166320761468546No Hit
GGGAAGACAAGAACAAGGGGACAGGGTTTCAGGCTGGGGAAACAGCACAT170.12166320761468546No Hit
GGGAGGAAATTCATCCTCCCGTGAACATACGCTACCACCACTATTATTGG170.12166320761468546No Hit
GGGTGAGCGGTCCAAGTCCGCAGGAGGCCAGAGGCTCTTTGTGGTTGAAT160.11450654834323339No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA160.11450654834323339No Hit
AGTAGTCCTTTTCCCTCTTTGTGCCCTTAAAAATACAGTTCAAGCTATAT160.11450654834323339No Hit
GGGAAAACACCAGTGTACTCAGCTAGTGGGCAGATCTTTGTAATGAAGAC160.11450654834323339No Hit
GGGAAAGACAAGAGCAGCTCCAAGGAGAGAAGAACGTCCAGAAAACCAAG160.11450654834323339No Hit
GGGTCCAGGCAGCTCCTTCCTGCAGGGGGCAGGGGAGAGGGCCACCAGGG160.11450654834323339No Hit
GGGGAGGTCTCAAGGAGATTTTACTCGTGGCAGAAGGCAAAGCAGGAGCA160.11450654834323339No Hit
GGGTCGTCGCCGCCACGGGACGGAGCGGGCGCTGAATGGGGCGGCTGAAG160.11450654834323339No Hit
GGGGAACTTGGGTTTTATTCCTACTCATGCATGATGTGTTTCAGAACTTA160.11450654834323339No Hit
GGGTGGAGAAACAGACATTCAATTCTATGAAACAGAACAGGAAACCCAGA160.11450654834323339No Hit
GGGATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCATCCG160.11450654834323339No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC160.11450654834323339No Hit
GGGTGGATAGGTAGGTAGATGGGTGCCTAGATAGGCACCCATCTACCTAC150.10734988907178128No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA150.10734988907178128No Hit
GGGGTCCATCGCCCTCCTGGGCTTGGTGGGCACAACTGTGTGCTCTGCTT150.10734988907178128No Hit
GGGGGGGGCCAACGTCCTGACTTTCGGGGCCGGCAGCAGGCTGACCGTGC150.10734988907178128No Hit
GGGGCTTCACCATGTTGGCCAGGCTGGTCTTGAACTCCTGGCCTCAAGCG150.10734988907178128No Hit
GGGGGGGTGGGTGTGCGGAGGGAAGCGCGCGGCGGCGCCGCCGCCGCCGC150.10734988907178128No Hit
GGGATTACAGGTGTGAGCCACGACGCCAGGCCCAATTACCGCTTTCTTAA150.10734988907178128No Hit
GGGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCAC150.10734988907178128No Hit
GGGATGTGGAGGCGTTTCTCTATGTCTTTCCAGCTGGAGGAATCAAGGTG140.1001932298003292No Hit
GGGGGAGGCAGTGGTCACAACTCTCCCCAGAGAAGGTGGTGTGAGGCCAT140.1001932298003292No Hit
GGGGGCAGGTGGGGCTCCATGGAGGGAGGGCTGAGGCTGAGGTGGGGGTA140.1001932298003292No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAA140.1001932298003292No Hit
GGGTTCAAGCAATCCCCTTGCCTCAGCCTCCTGAGCAGCTAGGACTACAG140.1001932298003292No Hit
GGGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGCTGCTT140.1001932298003292No Hit
GGGCAAAAACTGGAAGCATTTCCTTTGAAAACTGGCACAGGACAGGGATG140.1001932298003292No Hit
GGGGGAAGCAATATGAAATGATCTGCTGCAGTGCTCTGAGCCCTAGGATT140.1001932298003292No Hit
GGGGCAGCCCCTTGGAGGCAGGGGACAGTTGAGGTTCGAACACTTCTCCC140.1001932298003292No Hit
GGGGTAAAACTAACCTGTCTCACGACGGTCTAACCCAGCTCACGTTCCCT140.1001932298003292No Hit
GGGAAAGCGGGACCCGGGTGAGTTTTGAAAAACATAAGGGGAACGGGGGT140.1001932298003292No Hit
GGGGGGCAGAATACAGGTATGGCAGGGAGGGAAGGAGAAACTTAGGGGGA140.1001932298003292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)