FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953547

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953547
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13973
Sequences flagged as poor quality0
Sequence length290-300
%GC44

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC4122.9485436198382597No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA2171.5529950619051027No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1891.3526086023044444No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC1801.2881986688613754No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC1711.2237887354183068No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC1661.1880054390610464No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1631.16653546124669No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1581.1307521648894296No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1561.1164388463465256No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC1541.1021255278036213No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1120.8015458384026336No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA1090.7800758605882774No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC980.7013526086023044No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC950.6798826307879482No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC930.665569312245044No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC920.658412652973592No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC910.6512559937021398No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC900.6440993344306877No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC900.6440993344306877No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC890.6369426751592357No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA890.6369426751592357No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC860.6154726973448794No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC860.6154726973448794No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA850.6083160380734273No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA770.5510627639018106No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC770.5510627639018106No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA770.5510627639018106No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC760.5439061046303585No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC730.5224361268160023No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC710.5081228082730981No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC710.5081228082730981No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA660.4723395119158377No Hit
CGGATGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT660.4723395119158377No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA650.4651828526443856No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG640.45802619337293354No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANGGACAGGATTGACAGATTGA630.45086953410148145No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC600.4293995562871251No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA550.3936162599298647No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA530.3793029413869606No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA490.3506763043011522No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA480.3435196450297001No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA470.3363629857582481No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC470.3363629857582481No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA460.329206326486796No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA430.3077363486724397No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA430.3077363486724397No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA420.3005796894009876No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC410.2934230301295355No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA410.2934230301295355No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA410.2934230301295355No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC400.28626637085808343No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC400.28626637085808343No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC400.28626637085808343No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA380.27195305231517924No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA380.27195305231517924No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC340.24332641522937093No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC330.23616975595791884No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG330.23616975595791884No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC310.22185643741501468No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA300.21469977814356256No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC300.21469977814356256No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG300.21469977814356256No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC290.20754311887211052No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG280.2003864596006584No Hit
CGGATGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT280.2003864596006584No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG270.19322980032920634No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG260.18607314105775424No Hit
CTTGNGNGANNCNTCACCCGGCCNGGNCACGGACAGGATTGACAGATTGA250.17891648178630215No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA250.17891648178630215No Hit
CGGATGGTTNNGNATGAGGCCANGNTTAANAGGGACGGCCGGGGGCATTC250.17891648178630215No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA250.17891648178630215No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC240.17175982251485006No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC240.17175982251485006No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC240.17175982251485006No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC230.164603163243398No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG230.164603163243398No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC230.164603163243398No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA230.164603163243398No Hit
CGGATGGTTNNGNCCTAGCCTTNCNANTANNTCTTAGTAAGATTACACAT220.1574465039719459No Hit
CGGATGNTTNNGNCCTAGCCTTTNTANTANCTCTTAGTAAGATTACACAT220.1574465039719459No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC220.1574465039719459No Hit
CTTNGGGGANNCNTCACCCGGCNNNGNCANGGACAGGATTGACAGATTGA220.1574465039719459No Hit
CTTGGGGGANNCNTCACCCGGCNCNGNCANNGACAGGATTGACAGATTGA220.1574465039719459No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTTAGTAAGATTACACAT220.1574465039719459No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANNGACAGGATTGACAGATTGA210.1502898447004938No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA210.1502898447004938No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC210.1502898447004938No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG210.1502898447004938No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGTAAGGTGGAGTGGGTTTGGGG210.1502898447004938No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC200.14313318542904171No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC200.14313318542904171No Hit
CGGANGNTTNNGNCCTAGCCTTTNTANTAGCTCTNAGTAAGATTACACAT200.14313318542904171No Hit
CTTGGGNGANNCNTCACCCGGCCNGGNCANGGACAGGATTGACAGATTGA190.13597652615758962No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC190.13597652615758962No Hit
CTTNGTGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA180.12881986688613756No Hit
CGGATGGTTNNGNATTGTTCCANANATCTNTTAACACTGTTCAATTTTTT180.12881986688613756No Hit
CTTGGGGGANNCNTCACCCGGCNNNGNCANNGACAGGATTGACAGATTGA180.12881986688613756No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA180.12881986688613756No Hit
CTTGGGGGANNCNTCACCCGGCCNGGNCACGGACAGGATTGACAGATTGA170.12166320761468546No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC170.12166320761468546No Hit
CTTGGTNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA170.12166320761468546No Hit
CGGNTGGTTNNGNCCTAGCCTTNCNATTANCTCTTAGTAAGATTACACAT170.12166320761468546No Hit
CGGNTGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG170.12166320761468546No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC170.12166320761468546No Hit
CGGATGNTTNNGNATGAGGCCATNATNAANAGGGACGGCCGGGGGCATTC170.12166320761468546No Hit
CTTGGGGGANNCNTCACCCGGCCNNGNCACGGACAGGATTGACAGATTGA170.12166320761468546No Hit
CGGATGGTTNNGNCCTAGCCTTTNNANTAGCTCTTAGTAAGATTACACAT160.11450654834323339No Hit
CGGATGGTTNNGNTAAGGTTGTCTNGTAGNAAGGTGGAGTGGGTTTGGGG160.11450654834323339No Hit
CTTGGGGGANNCNTTCACTCTGNGNTGATNCCACTGCTTCCGCGGACAGG160.11450654834323339No Hit
CGGANGNTTNNGNATGAGGCCATNATNAAGAGGGNCGGCCGGGGGCATTC160.11450654834323339No Hit
CGGATGGTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG150.10734988907178128No Hit
CTTNGTGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA150.10734988907178128No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA150.10734988907178128No Hit
CTTNGGGGANNCNTCACCCGGCNCNGACANNGACAGGATTGACAGATTGA150.10734988907178128No Hit
CGGATGGTTNNGNTAAGGTTGTCNNGNAGNAAGGNGGAGTGGGTTTGGGG150.10734988907178128No Hit
CGGANGNTTNNGCTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG150.10734988907178128No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANCTCTTAGTAAGATTACACAT150.10734988907178128No Hit
CGGANGNTTNNGNTAAGGTTGTCNNGNAGNAAGGTGGAGTGGGTTTGGGG150.10734988907178128No Hit
CGGANGNTTTNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG150.10734988907178128No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC150.10734988907178128No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNNAGGTGGAGTGGGTTTGGGG150.10734988907178128No Hit
CTTGNGNGANNCCTCACCCGGCCNGGNCACGGACAGGATTGACAGATTGA140.1001932298003292No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACCTTGTTCA140.1001932298003292No Hit
CGGANGNTTNNGNATGAGGCCATNATNAAGAGGGACGGCCGGGGGCATTC140.1001932298003292No Hit
CGGNTGGTTNNGNCCTAGCCTTNNNANTANNTCTTAGTAAGATTACACAT140.1001932298003292No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC140.1001932298003292No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)