FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953548

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953548
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14474
Sequences flagged as poor quality0
Sequence length35-300
%GC48

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN9126.300953433743264No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC4623.1919303578831006No Hit
GGGAGACAAAGACAAGTGACAAGAATTAGAGGTTTAAAAAGCAACCAGAT1400.9672516236009396No Hit
GGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAGC990.6839850766892358No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG920.6356224955091889No Hit
GGGGAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAAAAG840.5803509741605638No Hit
GGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGTG800.5527152134862512No Hit
GGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCA790.5458062733176731No Hit
GGGGGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAAACAGC770.5319883929805168No Hit
GGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAACA760.5250794528119387No Hit
GGGGGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAGCTGTATT750.5181705126433604No Hit
GGGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA740.5112615724747823No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG720.49744369213762607No Hit
GGGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGAT600.4145364101146884No Hit
GGGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAAT590.4076274699461102No Hit
GGGAGATATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATG590.4076274699461102No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA560.38690064944037583No Hit
GGGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTG530.36617382893464145No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCAC520.3592648887660633No Hit
GGGAGGGAGACAGCGCTGTTATCAAGTGTACTTATTCAGACAGTGCCTCA520.3592648887660633No Hit
GGGAGAAACACAGTGGAAGATTAAGAGTCACGCTTGACACTTCCAAGAAA500.34544700842890697No Hit
GGGAGAGGTCCTGTTTCCCTGACAATCATGACTTTCAGTGAGAACACAAA500.34544700842890697No Hit
GGGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAAC490.33853806826032884No Hit
GGGGAGATCAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGC490.33853806826032884No Hit
GGGGACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAATT490.33853806826032884No Hit
GGGGAGAATGTGGAGCAGCATCCTTCAACCCTGAGTGTCCAGGAGGGAGA480.33162912809175077No Hit
GGGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATT470.3247201879231726No Hit
GGGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCT470.3247201879231726No Hit
GGGGGAGGACAGTGCCTGGAGAGGACCTGAACAAGGTGTTCCCACCCGAG460.31781124775459446No Hit
GGGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTT460.31781124775459446No Hit
GGGAGATATACAGCAACTCTGGATGCAGACACAAAGCAAAGCTCTCTGCA460.31781124775459446No Hit
GGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCA450.3109023075860163No Hit
GGGGATGTTAAGGATCAAGACCATTATTTGGGTAACACACTAAAGATGAA450.3109023075860163No Hit
GGGGGCTGAATTTAAGAAGAGTGAAACCTCCTTCCACCTGACGAAACCCT430.29708442724886003No Hit
GGGAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCACGCTCGA430.29708442724886003No Hit
GGGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGT410.2832665469117038No Hit
GGGGATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTGGGT410.2832665469117038No Hit
GGGGACTGACTAAGAAACACTGTGGGATGGATAGCAGCTATAAATTGATC400.2763576067431256No Hit
GGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAGG390.26944866657454747No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT380.26253972640596934No Hit
GGGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTAT370.25563078623739116No Hit
GGGGAGCTTGGAGAGGGGTGGGTACTGGAGAAGACCAGCCCCTTCGCCAA350.2418129059002349No Hit
GGGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACT350.2418129059002349No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG350.2418129059002349No Hit
GGGGATAACTGGAGGTTGCAGGTCAATGACTGATCTTAATTGGGAAGAAC340.23490396573165678No Hit
GGGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTC330.22799502556307863No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG330.22799502556307863No Hit
GGGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTT320.22108608539450048No Hit
GGGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCA320.22108608539450048No Hit
GGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAGC320.22108608539450048No Hit
GGGGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTA310.21417714522592232No Hit
GGGATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAA300.2072682050573442No Hit
GGGAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATTACTGTACTCC300.2072682050573442No Hit
GGGACTACGCTCACTGTGAAGCCAAATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC300.2072682050573442No Hit
GGGGATGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTG290.2003592648887661No Hit
GGGAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATCTTA290.2003592648887661No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT290.2003592648887661No Hit
GGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTAA280.19345032472018792No Hit
GGGGATTGCAGCTCAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGT280.19345032472018792No Hit
GGGGGGGCCTGGAACCTCAATGTGCACTTGAACAATGAAGTTGGTGACAA280.19345032472018792No Hit
GGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGGA280.19345032472018792No Hit
GGGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTG280.19345032472018792No Hit
GGGAGCCCAGAGAAGTCTGTTCCAGGACGGGCTCTTTCAGGAGCAGCTAA270.1865413845516098No Hit
GGGGGTTGAATACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAA270.1865413845516098No Hit
GGGGGCCATATCTCTAGGTCTTAGGTATGAAAAGGATGTGTGGGCTTCTG270.1865413845516098No Hit
GGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAA270.1865413845516098No Hit
GGGAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGGTGATTTTGTG270.1865413845516098No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG260.17963244438303164No Hit
GGGGACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGTCA260.17963244438303164No Hit
GGGGACACACCAGCTCCTCTGGGGTTTGCCACACTCATGATGCACTGTGT260.17963244438303164No Hit
GGGAGATTTGCAGCTTTCTAGGCAGGAGATAAGACAATCTGCATCTTCAC250.17272350421445348No Hit
GGGACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACTCTT250.17272350421445348No Hit
GGGAGTCTTTCTTCTGGTACAGACAGGATTGCAGGAAAGAACCTAAGTTG250.17272350421445348No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT240.16581456404587538No Hit
GGGGAGAGAAGGTGGTGTGAGGCCATCACGGAAGATGCTGCTGCTTCTGC240.16581456404587538No Hit
GGGAAAGTATCTCTGCTATGTTCATTTCTTTTTGGATTGAAAATTTTAAT230.15890562387729723No Hit
GGGGAGTATAGGCTCCCTAGACCTGCTCCCCAGCTGTGGTGTAAAAATGG230.15890562387729723No Hit
GGGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGAC230.15890562387729723No Hit
GGGAGAAAGACTAGGGATTCACCCAGTAAAGAGAGCTCATCTGTGACTGA230.15890562387729723No Hit
GGGGAGTGCTTTGCCAACCTGGCCTGTTTGATCTGGTTTTTGTTGTTGAG230.15890562387729723No Hit
GGGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACG230.15890562387729723No Hit
GGGGGAAAATGATGCTGTAAAATGGAAATGAGACCTTTAGAAAGAGAGAT220.15199668370871908No Hit
GGGACTGTGATTTCTTCATGTTAAGGATCAAGACCATTATTTGGGTAACA220.15199668370871908No Hit
GGGGGTATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACT220.15199668370871908No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG220.15199668370871908No Hit
GGGGGCAGTAGAGTCTGGAGAGTTTAGAATGATGGTTTTTGCAATGACTT210.14508774354014095No Hit
GGGGAAAGAGAGCTCATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTC210.14508774354014095No Hit
GGGATTATTTGGGTAACACACTAAAGATGAACTATTCTCCAGGCTTAGTA200.1381788033715628No Hit
GGGGGAACTAAGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAAT200.1381788033715628No Hit
GGGGAAAGCAGATTCTTTTTATGATTTTTAAAGTAGAAATATCCATTCCA200.1381788033715628No Hit
GGGATAAGATTTCAGTTCTCAGTGAGTCTAAGTGACAGAAGGAATGGAGA200.1381788033715628No Hit
GGGAGAGCAGGAGACTTTTCACTCTGCAGGGGAGCGCTGTCAGCATGACA200.1381788033715628No Hit
GGGAGTTTATGTAAAGGGTTGGCCCAGAGTGTGTATTCAGGAGGAGGTGC200.1381788033715628No Hit
GGGGGATGATGGAAGTAGCTCTTATGGCTGGAGATTGCAGGTTTATGACT200.1381788033715628No Hit
GGGGAGAAGAGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGC200.1381788033715628No Hit
GGGGGACTTGATTATGAAATGCTACCAGTTTGAAGCAGTCACTGAAAAAC190.13126986320298467No Hit
GGGGAAATTGAAACCTGCCTGATGTGGGATGTGCTGTGGCTGCTGCTTTG190.13126986320298467No Hit
GGGAGACTGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCA190.13126986320298467No Hit
GGGATCTTCACAGGAGGGATGGCCATGCTCCTGGGGGCATCAGTGCTGAT190.13126986320298467No Hit
GGGGGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAGGAAC190.13126986320298467No Hit
GGGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGAATTATCGTAAT190.13126986320298467No Hit
GGGAGTTTCTTCTTCCTGCAGCTGGTTGAGTTCTTTCCAGTCCCCCGGCC180.12436092303440652No Hit
GGGAACACACTAAAGATGAACTATTCTCCAGGCTTAGTATCTCTGATACT180.12436092303440652No Hit
GGGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGT180.12436092303440652No Hit
GGGAGGACAGATTTCTTTTATGATTCCTACAGCAGAAAAATGAGAAACGT180.12436092303440652No Hit
GGGGAAATTCTTTAAACCTAGAATCAGACACAAAAACTGAACTCTGGGTC180.12436092303440652No Hit
GGGACACAAGTCAACTTCTGGGAGCAGATCTCTGCAGAATAAAAATGAAA170.11745198286582839No Hit
GGGAGGCAGGAAGCCGGACCTCGGGGGACGCAGCAGATATCCAGAACCCT170.11745198286582839No Hit
GGGACTGTCGCTTTCAACTGTACTTACAGCAACAGTGCTTCTCAGTCTTT170.11745198286582839No Hit
GGGGATAGAAGTAACTCTTATAACTGGAGGTTGCAGGTCAATGACTGATC170.11745198286582839No Hit
GGGGAGGTATGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTA170.11745198286582839No Hit
GGGATTCAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCAT170.11745198286582839No Hit
GGGGTTGAAAAGGATGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAA160.11054304269725024No Hit
GGGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTAT160.11054304269725024No Hit
GGGGGTGTGGGCTTCTGGGTGTTTTTGACTGACTAAGAAACACTGTGGGA160.11054304269725024No Hit
GGGGGCAGCTTTCTAGGCAGGAGACAAGACAATCTGCATCTTCACAGGAG160.11054304269725024No Hit
GGGATTTGTCCTTGAAGTATGAGGCTGGTGGCAAGAGTAACTGTGTTTCT160.11054304269725024No Hit
GGGAGGACAGATTCTTTTTATGATTCCTAAAGTGGAAGAAATAAAGTATC160.11054304269725024No Hit
GGGAGTATCACTGTGTCTTATAACACCGACAAGCTCATCTTTGGGACTGG160.11054304269725024No Hit
GGGATCTGTGACTGAGGAGCCTTGCTCCATTTCAGGTCTTCTGTGATTTC160.11054304269725024No Hit
GGGGAATATGAAGGCTGCAGCGATGCCTGCAAGTCAGCTTTTGCTACACT160.11054304269725024No Hit
GGGAGTCATTTGGTGCCAGTGACAGGGAAGAGCAGAGGGGCTTAGGAGGT160.11054304269725024No Hit
GGGGGTTTTTCTGCTGTGGGTACGTGAGCAGGAAACATGGAGAAGAATCC160.11054304269725024No Hit
GGGGGAGTTTCCTTCCCTCACCCACATGAAGTGTCTACCTTCTGCAGACT160.11054304269725024No Hit
GGGGGTTCATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCA150.1036341025286721No Hit
GGGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGG150.1036341025286721No Hit
GGGAGACACAGCAAAAGAGCCTAGAACCTGGGTCCTAGTTTGCACCTAGA150.1036341025286721No Hit
GGGGGTTGAGCTTCCTATCACAGTGGAACACCGGTAACCAGTTCTATTTT150.1036341025286721No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG150.1036341025286721No Hit
GGGGAGGCTTTGTCGGGTGGAGCTGATTGGTTGCAGGAGCAGCAACAGTT150.1036341025286721No Hit
GGGGATGAACTAAGATACAGAAGTGGCGCCTCTGAGAAAAGAAGGTTGGA150.1036341025286721No Hit
GGGGAGGAGGAGGTGCTGACGGACTCACCTTTGGCAAAGGGACTCATCTA150.1036341025286721No Hit
GGGGATATGTAAAATGAAGGGTCTGTGGAAGGACATGAATAAAGCACAGG150.1036341025286721No Hit
GGGAGTTTGACGCTGTGTGGAATATGGAAACAAGCTGGTCTTTGGCGCAG150.1036341025286721No Hit
GGGAGAGTGTTTATCTTGTGAGCCATTCTCCATATTTCAGATATAAGATT150.1036341025286721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)