FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953550

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953550
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27472
Sequences flagged as poor quality0
Sequence length35-300
%GC49

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN961434.995631916132794No Hit
GGGAGCGCCCGAATCTGGCTTGGCGGAATATCTCTTTGACAAGCACACCC769828.021258008153755No Hit
GGGAGAGCGGGCTGAATGCAATGGAGTGTGCATTACATTTGGAAAAAAAT5151.8746359930110659No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG4541.6525917297612114No Hit
GGGGTGACTTCCCTGGTCACCAAGGCAGTGCATGCATGTTGGGGTTTCCT3711.3504659289458356No Hit
GGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACGA3001.0920209668025627No Hit
GGGGATGCTTGCATGTGTAATCTTACTAAGAGCTAATAGAAAGGCTAGGACCAAACCATCCG2280.8299359347699476No Hit
GGGGTTCAACGGGTTACCCGCGCCTGCCGGCGTAGGGTAGGCACACGCTG2130.7753348864298195No Hit
GGGACTGAGAAGGCTCACGAAGGGCATCCGCAATGTTGGTTTCACTGAGA1060.38584740827023883No Hit
GGGGAGAACAGCGTCTTTTACCTGTCACCGAAGCCAGGAAGCCCCGTTTG1060.38584740827023883No Hit
GGGGAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCACG1030.3749271986022132No Hit
GGGTTTGATTCCTTTCAATTCCATTCCATTCGGGTTGATTATATTCCATT990.3603669190448457No Hit
GGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG880.320326150262085No Hit
GGGAAAAAACATTCAGTACTTCGGCGCCGGGACCCGGCTCTCAGTGCTGG780.2839254513686663No Hit
GGGGAGACTGGATGTGTTTTTGACAGGATATGTAACACAGTGTGATTTAT720.262085032032615No Hit
GGGGAGAAGGACCTCTTTCTAACAGAGGTCCTTCTCTCCATATATGCTCT690.25116482236458937No Hit
GGGTTGATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTCCATTC640.23296447291788003No Hit
GGGGGCACTGCCAGTAAACTCACCTTTGGGACTGGAACAAGACTTCAGGT640.23296447291788003No Hit
GGGATGGCTACACCTTCGGTTCGGGGACCAGGTTAACCGTTGTAGAGGAC620.22568433313919625No Hit
CGGGACCTGGGCCTGGTAGACAGGATGAGAAGATTTTTGAGCCTGAGATC610.2220442632498544No Hit
GGGGTTTCCTTTACCTTTTCTATAAGTTGTACCAAACCATCCG610.2220442632498544No Hit
GGGGCAGACAGCCCTGAGATCACCTAAAAAGCTGCTACCAAGACAGCCAC570.2074839836924869No Hit
GGGAATTCGAGTCCATTGCATTCCTTTCAATTCCATTCCATTCCATTCCA570.2074839836924869No Hit
GGGGCTAAACCTAGCCCCAAACCCACTCCACCTTACTACCAGACAACCTTAACCAAACCATCCG560.20384391380314504No Hit
GGGTTCAATTCCTCTTCTTAACAACATACCCATGGCCAACCTCCTACTCC560.20384391380314504No Hit
GGGGAATAATTGCAATCCCCGATCCCCATCACGAATGGGGTTCAACGGGT560.20384391380314504No Hit
GGGTTAATTCCATTCCATTCCATTCGATTGCAATCGAGTTGATTCCATTC550.20020384391380314No Hit
GGGATTCAATTCCATTCTATTCCACTCCATTCTATTCCATTTCATTCAAT550.20020384391380314No Hit
GGGAGGAGGAAGCTACATACCTACATTTGGAAGAGGAACCAGCCTTATTG550.20020384391380314No Hit
GGGGCAAAATAGTGGGAAGATTTATAGGTAGAGGCGACAAACCTACCAAC520.18928363424577752No Hit
GGAGAGGTTTGAGGGTGCTTAAATAGGTAAAGTCTGGGGAAGCTTACCAG520.18928363424577752No Hit
GGGCAAAAAGCAGCTAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATCA500.18200349446709377No Hit
GGGGAAACAGATGCTGTAGCGAAGTCACCATCTGTTTCCTCTTGCACACA500.18200349446709377No Hit
GGGACATATTTCCTCTTCTTCTACCTTTAGCTTTTATTCCACAGCTCTTG490.1783634245777519No Hit
GGGAAACATATACAGGACAGAATATGGACTGAAAGTGTGGAGAATAAACA480.17472335468841002No Hit
GGGTTCCATTGCACTCCACTCCATGCCTTTCAATTCCACACCACTCCACT470.17108328479906815No Hit
GGGGGGAATGATTCCATTCCATTCCACTCCATTCCAATTGGGTTGAATCC460.16744321490972627No Hit
GGGACAGGCAAACTGATCTTTGGGCAAGGGACAACTTTACAAGTAAAACC440.1601630751310425No Hit
GGGGAAACCAAAAGAAGCCTCCAGACAGCCCTGAGATCACCTAAAAAGCT440.1601630751310425No Hit
GGGGAGTCACTGCATGTTCAGGAGGGAGACAGCACCAATTTCACCTGCAG430.15652300524170065No Hit
GGGGGTTTTTGTCCTGGGCCTCCAGGCTGTGAGCACAGATACGCAGTATT400.145602795573675No Hit
GGGGATTTTGGCCATGGCTTTGCAGAGCACTCTGGGGGCGGTGTGGCTAG400.145602795573675No Hit
GGGGAATGACTGATCTTAATTGGGAAGAACAAGGATGACATCCATTCGAG400.145602795573675No Hit
GGGAGGAAGGAGGGAAGGAAAGAAGGAAGTCAGGAAGGAAGGAAGGAAGG400.145602795573675No Hit
GGGCCACTCCACCTTACTACCAGACAACCTTAGCCAAACCATCCG380.13832265579499126No Hit
GGGGGACTATGAAATCCACGATGGGATGAACCTGGAGCTTTATTATCAAT380.13832265579499126No Hit
GGGCCAACCTCCTACTCCTCATTGTACCCATTCTAATCGCAATGGCATTC380.13832265579499126No Hit
GGGCATTGTACCCATTCTAATCGCAATGGCATTCCTAATGCTTACCGAAC370.1346825859056494No Hit
GGGGTGGAGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGC370.1346825859056494No Hit
GGGAAGCAGGCACGTCCGGAAGCGCTCCTCCAGCAGGGACAGCTCGCTGA350.12740244612696563No Hit
GGGGGGAACACTGAAGCTTTCTTTGGACAAGGCACCAGACTCACAGTTGT340.12376237623762376No Hit
GGGTATTTAGTGGGTCATTTCAGTATATGTCATATATGAATGAGACTATT340.12376237623762376No Hit
GGGAGCGCCCGAATCTGGCTTGGCGAAATATCTCTTTGACAAGCACACCC340.12376237623762376No Hit
GGGCCGGAGCAGGAACAGGTTGAACAGTCTACCCTCCCTTAGCAGGGAAC340.12376237623762376No Hit
GGGATTGCAGACACCCAGACTGGGGACTCAGCTATCTACTTCTGTGCAGA340.12376237623762376No Hit
GGGTGCAAGGAGGGGAAATGCGTGATGCTTATAAAACCATCAGATCTCAT340.12376237623762376No Hit
GGGGGAGCTTTAATTTATTAATGCAAACAGTACCTAACAAACCCACAGGTCCTAAACTACCAAACCATCCG330.12012230634828187No Hit
GGGAGACGAGCAGTACTTCGGGCCGGGCACCAGGCTCACGGTCACAGAGG330.12012230634828187No Hit
GGGAAAATACAACCCAGGTGTCACTCCTTCCAGGAATTCTCCATTGCCCC330.12012230634828187No Hit
GGGAAAAACATCCTGAGGACAGTGCCTGGAGAGGACCTGAAAAACGTGTT330.12012230634828187No Hit
GGGAGGCTTCTCACCCTGCAGCAGGGACCTGTGAGCATGGCATGCCCTGG330.12012230634828187No Hit
GGGAAACTGCCGCACACTTTTAAATCATCAGATCTCGTGAGAACTCACTA320.11648223645894001No Hit
GGGAGGATTTGAAATTCGGAATCCACAGACCCCAGGGATTAACTGCAGTT320.11648223645894001No Hit
GGGGGATCAGGAAGCGCCAGAGGCTGCGTTGAGGAAGGAGGTGCTGGATG320.11648223645894001No Hit
GGGACCAGACTGCTGGTCAGGCCTGATATCCAGAACCCTGACCCTGCCGTGTACCAGCTGAGAGAC320.11648223645894001No Hit
GGGAAGGGTCTTGTGTTTTAATGCCAATAAATGTACCAGCTGAGAGAC320.11648223645894001No Hit
GGGGACCTTTGATACCACCCTTAAACAGAGTTTGTTTCATATCACAGCCT320.11648223645894001No Hit
GGGCTGATGTTCGCCGACCGTTGACTATTCTCTACAAACCACAAAGACAT320.11648223645894001No Hit
GGGAGAACTGCCCCCATGATCCAATCACCTCCCACCAGGTCCCTCCCTCA310.11284216656959813No Hit
GGGATAACAGGCGTGAGCCGCTGCGCCCAGCCTCAAATCCTTTTATATTG310.11284216656959813No Hit
ATTATTTTTAACTCACCTGACTGAATTGGAAGGGTACCGTACTGATTAAT300.10920209668025627No Hit
GGGACCACGTGTCTCTGGGGGACTATGAAATCCACGATGGGATGAACCTG300.10920209668025627No Hit
GGGAAGATGGACAAGGAAGAGACCAAAGACTGGATCCTTCCCTCAGACTA290.10556202679091438No Hit
GGGAGATCTCTGCAGAATAAAAATGAAAAAGCATCTGACGACCTTCTTGG290.10556202679091438No Hit
GGGGTGGAACTTCAATGTGCACTTGAACAATGAAGTTGGTGACAAGCATT290.10556202679091438No Hit
GGGTGTGGGCATCTGATATGGTTTGGCTGTGTCCCCACCCAAATCTCATA280.10192195690157252No Hit
GGGTGACCACGTCACCTCTTCACCGCACCCTCGGACTGCCCCAAGGCAGT280.10192195690157252No Hit
GGGGATATGCCTTCTAATGGCAAAGATTTCTGTAGGACCCAGAGTCTTGT280.10192195690157252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)