FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004953551

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004953551
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences27472
Sequences flagged as poor quality0
Sequence length290-300
%GC45

[FAIL]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per tile quality graph

[FAIL]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[FAIL]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CGGATGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC9343.3998252766453114No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC6782.4679673849737913No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC4431.6125509609784507No Hit
CGGANGNTTNNGNACAACTTATANAANAGGTAAANGAAACCCCAACATGC4071.4815084449621434No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNNAAAGGAAACCCCAACATGC3681.3395457192778102No Hit
CGGATGNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC3681.3395457192778102No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA3621.317705299941759No Hit
CGGATGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC3581.3031450203843913No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNTAAAGGAAACCCCAACATGC3441.2521840419336052No Hit
CGGATGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC3221.1721025043680837No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC2801.019219569015725No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTNCTTCCGCGGACAGGC2720.9900990099009901No Hit
TGCTNCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC2700.9828188701223064No Hit
TGCTTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC2540.9245777518928364No Hit
CGGATGGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC2470.8990972626674433No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANNACTGCTTCCGCGGACAGGC2470.8990972626674433No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANCACTGCTTCCGCGGACAGGC2310.8408561444379733No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTANCACTGCTTCCGCGGACAGGC2290.8335760046592894No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC2220.8080955154338962No Hit
CGGANGNTTNNGNACAACTTATANNANAGNTAAAGGAAACCCCAACATGC2020.7352941176470588No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC1980.7207338380896914No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGNNAAAGGAAACCCCAACATGC1940.7061735585323238No Hit
TGCTTCTGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1940.7061735585323238No Hit
CGGATGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1910.6952533488642982No Hit
CGGATGNTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1870.6806930693069307No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA1800.6552125800815376No Hit
CGGATGGTTNNGNACAACTTATNGNANAGNTAAAGGAAACCCCAACATGC1780.6479324403028538No Hit
CGGANGNTTNNGTACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1760.64065230052417No Hit
CGGNTGGTTNNGNACAACTTATNNNANAGGTAAAGGAAACCCCAACATGC1700.6188118811881188No Hit
CGGATGGTTNNGNACAACTTATNGCAAAGNTAAAGGAAACCCCAACATGC1700.6188118811881188No Hit
CGGATGGTTNNGNACAACTTATANNANAGNTAAANGAAACCCCAACATGC1670.6078916715200932No Hit
CGGNTGGTTNNGNACAACTTATNGNAAAGNNAAAGGAAACCCCAACATGC1630.5933313919627257No Hit
CGGATGGTTNNGNACAACTTATCGNAAAGNTAAAGGAAACCCCAACATGC1540.5605707629586487No Hit
TGCTTCNGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1490.5423704135119395No Hit
CGGATGNTTNNGNACAACTTATNNNANAGGTAAANGAAACCCCAACATGC1460.5314502038439137No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC1450.5278101339545719No Hit
CGGANGNTTTNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC1390.5059697146185207No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANNGCTTCCGCGGACAGGCTAA1370.4986895748398369No Hit
TGCTTCNGANNGCTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1370.4986895748398369No Hit
TGCTTCTGANNGNTCACTCTGCNTNGNTANCACTGCTTCCGCGGACAGGC1360.49504950495049505No Hit
CTTGNGNGANNCNCTCTGCGTTGNTANCACTGCTNCCGCGGACAGGCTAA1330.48412929528246945No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTANNACTGCTTCCGCGGACAGGC1330.48412929528246945No Hit
TGCTTCTGANNGNTCACTCTGCNTNGATANNACTGCTTCCGCGGACAGGC1330.48412929528246945No Hit
CGGATGNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1310.47684915550378565No Hit
CTTGGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA1290.4695690157251019No Hit
TGCTTCTGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1290.4695690157251019No Hit
TGCNTCTGANNGNTCACTCTGCNTNGATANCACTGCTTCCGCGGACAGGC1260.45864880605707636No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTGCTTCCGCGGACAGGC1250.4550087361677344No Hit
TGCTTCTGANNGNTCACTCTGCNTTGATANCACTGCTTCCGCGGACAGGC1210.4404484566103669No Hit
TGCTTCTGANNGNTCACTCTGCGTNGATANCACTGCTTCCGCGGACAGGC1200.43680838672102507No Hit
TGCNTCTGANNGNTCACTCTGCNNNGNTACCACTGCTTCCGCGGACAGGC1150.41860803727431567No Hit
TGCTNCNGATNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1120.4076878276062901No Hit
TGCTNCNGANNGNTCACTCTGCGNNGNTANCACTNCTTCCGCGGACAGGC1110.4040477577169481No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANTGCTTCCGCGGACAGGCTAA1100.4004076878276063No Hit
TGCTNCTGANNGNTCACTCTGCGNNGNTACCACTGCTTCCGCGGACAGGC1070.3894874781595807No Hit
CTTGGGNGANNCNCTCTGCGTTGNTANCANTGCTTCCGCGGACAGGCTAA1040.37856726849155503No Hit
TGCTNCNGANNGCTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC1030.3749271986022132No Hit
TGCTTCNGANNGNTCACTCTGCNNNGNTACCACTNCTTCCGCGGACAGGC1010.3676470588235294No Hit
CGGANGGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC1010.3676470588235294No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA880.320326150262085No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTTCCGCGGACAGGCTAA820.2984857309260338No Hit
CTTGGGGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA730.26572510192195686No Hit
CGGANTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC690.25116482236458937No Hit
CTTGGGGGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA670.24388468258590568No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGTAGNAAGGTGGAGTGGGTTTGGGG670.24388468258590568No Hit
CTTGGGGGANNCNCTCTGCGTTNANANCANTGCTTCCGCGGACAGGCTAA640.23296447291788003No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCANNGCTTCCGCGGACAGGCTAA640.23296447291788003No Hit
CTTGGGNGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA640.23296447291788003No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANTGCTTCCGCGGACAGGCTAA620.22568433313919625No Hit
CTTGNGNGANNCCCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA610.2220442632498544No Hit
CGGATTGTTNNGNACAACTTATANNANAGGTAAAGGAAACCCCAACATGC600.21840419336051253No Hit
CTTGGGGGANNCNCTCTGCGTTGANACCANTGCTTCCGCGGACAGGCTAA580.21112405358182876No Hit
CTTGGGGGANNCNCTCTGCGTTNATACCANTGCTTCCGCGGACAGGCTAA580.21112405358182876No Hit
CGGATTGTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC570.2074839836924869No Hit
CTTGNGNGANNCNCTCTGCGTTGNNANCANTGCTNCCGCGGACAGGCTAA550.20020384391380314No Hit
CTTGGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA530.19292370413511942No Hit
CGGATGGTTNNGNTGGTGTCGTNTNGTCCNGAGAGTCCAGTTTGTATCTG520.18928363424577752No Hit
CGGATTNTTNNGTACAACTTATANNANAGGTAAAGGAAACCCCAACATGC510.18564356435643564No Hit
CTTGGGNGANNCNCTCTGCGTTNNNANCACTGCTNCCGCGGACAGGCTAA500.18200349446709377No Hit
CTTNGGGGANNCNCTCTGCGTTNNNANCACTGCTTCCGCGGACAGGCTAA490.1783634245777519No Hit
CTTGNGNGACNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA490.1783634245777519No Hit
TGCTTCNGANNGNTCACTCTGCGNTGNTACCACTGCTTCCGCGGACAGGC450.1638031450203844No Hit
CTTGGTGGANNCNCTCTGCGTTGNTANCACTGCTTCCGCGGACAGGCTAA430.15652300524170065No Hit
CGGATTNTTNNGNACAACTTATANAANAGNTAAAGGAAACCCCAACATGC430.15652300524170065No Hit
CGGATGGTTNNGNATAACTTATNGNAAAGNTAAAGGAAACCCCAACATGC420.15288293535235875No Hit
CTTGGGNGANNCCCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA400.145602795573675No Hit
TGCTTCTGANNGNTCAAACACANCNACCTNGGGTGGGAACACGTTTTTCA400.145602795573675No Hit
CTTNGGGGANNCNCTCTGCGTTNANACCANNGCTTCCGCGGACAGGCTAA400.145602795573675No Hit
CGGATGGTTNNGNTAAGGTTGTNNNGNAGNNAGGTGGAGTGGGTTTGGGG390.14196272568433316No Hit
CTTGGGGGANNCNTCACCCGGCNCNGACANGGACAGGATTGACAGATTGA390.14196272568433316No Hit
GTCTCTCAGNNGNTACACGGCANGNTCAGNGTTCTGGATATTGGGTTTCA390.14196272568433316No Hit
CGGNTGGTTNNGNTAAGGTTGTNNNGNAGNAAGGTGGAGTGGGTTTGGGG380.13832265579499126No Hit
CGGATTNTTNNGNACAACTTATANAANAGGTAAAGGAAACCCCAACATGC370.1346825859056494No Hit
CTTGGTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA360.1310425160163075No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGNGGAGTGGGTTTGGGG350.12740244612696563No Hit
CTTGNTGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA310.11284216656959813No Hit
CGGATGGTTNNGNTGGTGTCGTNNNGNCCNNAGAGTCCAGTTTGTATCTG300.10920209668025627No Hit
CGGATGGTTNNGNTAAGGTTGTNTNGNAGNNAGGTGGAGTGGGTTTGGGG300.10920209668025627No Hit
CTTGGGGGANNCNTCTGCATCANTNTGCGNTGATACCACTGCTTCCGCGG300.10920209668025627No Hit
CGGATGNTTNNGNTAAGGTTGTCNGGNAGNAAGGTGGAGTGGGTTTGGGG290.10556202679091438No Hit
CTTGNGGGANNCNCTCTGCGTTGNNANCACTGCTTCCGCGGACAGGCTAA280.10192195690157252No Hit
CGGANGNTTNNGNTAAGGTTGTCNGGNAGTAAGGTGGAGTGGGTTTGGGG280.10192195690157252No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGAGTCCAGTTTGTATCTG280.10192195690157252No Hit
CGGANGNTTNNGNTGGTGTCGTTNTGNCCTGAGANTCCAGTTTGTATCTG280.10192195690157252No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)